This study examines dog saliva from the perspective of Prophetic hadith and modern microbiology, focusing on purification using water and soil as guided by Prophet Muhammad ﷺ, highlighting the harmony between Islamic jurisprudence and science. A qualitative library research integrated Islamic textual analysis and microbiological literature review, analyzing ten authentic hadith, five classical fiqh sources, and twenty-five peer-reviewed studies (2015–2024). Data were processed through reduction, descriptive display, and comparative integration. Findings show that dog saliva contains pathogenic microorganisms, including Capnocytophaga canimorsus and rabies virus. The Prophetic practice of washing vessels seven times, once with soil, aligns with science, as soil harbors antibacterial agents like Streptomyces that neutralize resistant pathogens, validating the method both religiously and scientifically. These findings reinforce adherence to Prophetic guidance, provide scientific justification and emphasize hygienic practices for public health. The study’s integrative approach uniquely synthesizes hadith analysis with microbiological evidence, demonstrating the convergence of faith and science.