Biogas is one of the alternative energy sources that can be updated because it uses raw materials derived from livestock manure. The research aims to analyze biogas production from a mixture of odot grass and cow manure. The study was conducted with 3 treatments namely 100% cow manure (control), 70% cow manure + 30% odot grass, and 50% cow manure + 50% odot grass. The parameters are temperature, degree of acidity, total solid and volatile solids, volume biogas, flame test, and C and N content measurement. The research design used was a Completely Randomized Design (CRD) Each treatment was repeated three times. The results showed that the mixture of cow manure biogas with odot grass was very real (P<0.05) against the production of biogas produced successive production values for M₁₀₀G₀, M₇₀G₃₀, and M₃₀G₃₀ 191.3500 mL; 99,0067 mL and 0.0000 mL. The C/N ratio of each treatment is 27.52; 25.32 and 23.85. The average daily temperature is about the same for all treatments which is 27-29°C. The pH value of each treatment is 5.90-7.27; 5.00-7.00 and 4.77-6.70. The content of TS, VS the initial and final of each treatment is the initial TS of 11,014; 11,007; and 11,195; final TS 6,960; 6,794; and 6,714; initial VS 8,838; 8,965; and 9,309; final VS of 5734; 5,728; and 5,831. Biogas flames with a mixture of odot grass (M₁₀₀G₀) dan (M₇₀G₃₀) produce blue biogas flames (M₅₀G₅₀) do not produce flames. A mixture of odot grass and cow dung as a substrate did not produce high levels of biogas, compared to the non-odot grass mixture, which produced higher biogas production