Thai tea is one of the drinks that are popular with urban people. However, the less hygienic processing and presentation can be a source of microbiological contamination. This study aims to detect the presence of Escherichia coli in Thai tea drinks sold on Jalan Ayahanda, Medan, using the Most Probable Number (MPN) method. The research design was quantitative descriptive with a laboratory experimental approach, using ten samples from Thai tea sellers. The results showed that all samples were positive for coliform with an MPN value of 15–460 MPN/100 mL, eight samples were positive for the BGLB confirmatory test, and one sample (10%) was positive for Escherichia coli in EMB media. Based on the SNI standard (0/100 mL), the positive sample was declared unfit for consumption. These findings confirm the importance of implementing good hygiene and sanitation in the processing and sale of beverages to ensure food safety. They also contribute to the limited body of knowledge on street-level food safety risks in densely populated urban corridors and highlight the urgent need for strengthened street-food hygiene monitoring and consumer awareness to prevent microbial foodborne illness.