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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Pharmaciana: Jurnal Kefarmasian Indonesian Journal of Biotechnology Biology, Medicine, & Natural Product Chemistry Majalah Obat Tradisional The Indonesian Biomedical Journal Majalah Farmaseutik Indonesian Journal of Chemistry Indonesian Contemporary Nursing Journal (ICON Journal) Indonesian Journal of Cardiology Journal of Community Empowerment for Health Devotion: Journal of Research and Community Service The Avicenna Medical Journal Indonesian Journal of Pharmacology and Therapy
Dwi Aris Agung Nugrahaningsih
*) Department Of Pharmacology And Therapy, Faculty Of Medicine, Public Health And Nursing, Universitas Gadjah Mada; *) Herbal Medical Center, Faculty Of Medicine, Public Health, And Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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Journal : Pharmaciana: Jurnal Kefarmasian

 1 
Fibrotic effect of monoculture, conditioned media and coculture of fibroblast NIH3T3 cells on collagen secretion and mRNA TGF-beta expressions Rizki Awaluddin; Dwi Aris Agung Nugrahaningsih; Prasetyastuti Prasetyastuti
Pharmaciana Vol 13, No 2 (2023): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v13i2.25482

Abstract

Fibrosis is common profound in diabetes condition, they characterized by accumulation of extracellular matrix in tissue then cause tissue injury. The study aim to investigate effect of monoculture, conditioned media, and coculture of NIH3T3 cells in LPS-high glucose media conditions on mRNA TGF-B and collagen secretion. The fibroblast NIH3T3 were cultured with various culture type there is mono culture NIH3T3, conditioned media from RAW264.7 culture media, and coculture with RAW264.7 cells (ratio 1:1 and 1:2)  in normal media (media containing 4500 mikro g/mL of glucose) and HG-LPS media (media containing LPS 0.25 mikro g/mL and D-glucose 5400 mikro g/mL). Our study shows that mRNA TGF-B and collagen secretion of fibroblast NIH3T3 cells in monoculture and conditioned media from inflammatory macrophages are not statistically different. On other hand, the TGF-B mRNA expression and the relative ratio of collagen secretion in RAW 264.7 and NIH 3T3 cell coculture media with a culture ratio of 1:1 were the highest compared to other models (p kurang dari 0.05). Our study conclude that RAW 264.7 and NIH 3T3 coculture cells (1:1) in LPS and high glucose media is appropriate for fibrosis in vitro models.
Co-Authors Abdul Karim Zulkarnain Ade Saputri Ade Saputri ADITYA NUGRAHA Aditya Nugraha Ahmad Hamim Sadewa Ajib Diptyanusa Akhmad Kharis Nugroho Amira Fawwaz Tsabitah Andina Setyawati, Andina Andrew Limavanady Andrew Limavanady Ani Kristiyani Ari Wibowo Astika Widy Utomo, Astika Widy Atmaja, Sarah Aurelia Priscilla Regita Putri Budi Mulyaningsih Budi Mulyaningsih Buftheim, Sonia Caroline Caroline Christantie Effendy Christine Patramurti Denny Agustiningsih Dimas Adhi Pradana Dwi Liliek Kusindarta eko purnomo Eko Purnomo Ellsya Angeline Rawar Emilia Vivi Arsita Erna Kristin Ernaningsih Ernaningsih Eti Nurwening Sholikhah Eti Nurwening Sholikhah Foe, Kuncoro Gloria Evita Thalia Gloria Evita Thalia Hendy Wijaya Hera Nirwati Herzan Marjawan Hevi Wihadmadyatami I Made Andika Bara Kusuma Ibeneme, Sam Ikhsan Nur Salim Ikhsan Nur Salim Ismawati, Yuyun Iwan Dwiprahasto Januar Rizqi Jonathan J Jonathan J Jumina Jumina Jumina Jusak Nugraha Katharina Oginawati Ketut Shri Satya Wiwekananda Kwok, Yasmeen Mae Sri Hartati Wahyuningsih Mahatma Sotya Bawono Mia Munawaroh Yuniyanti Mia Munawarroh Yuniyanti Mia Munawarroh Yuniyanti Mosa Rini Nurul Hidayati Mosa Rini Nurul Hidayati Mubarika Haryana, Sofia Mustofa Mustofa Mustofa Mustofa Mustofa Mustofa Ngatidjan Ngatidjan Nico Jafet Nilasari, Fita Nur Arfian Oryza Chrisantia Pamungkas Bagus Satriyo Prasetyastuti Prasetyastuti Pratiwi, Woro Rukmi Probosuseno Probosuseno Raden Sumiharto Rahman, Intan Nur’azizah Rahmi Ayu Wijayaningsih Rahmi Ayu Wijayaningsih Rahmi Ayu Wijayaningsih Rasya Mayora Rasya Mayora RATNA SARI Ratna Sari Rizki Awaluddin Rocca, Matteo Ronny Martien Rul Afiyah Syarif Satriyo, Pamungkas Bagus Senny Yesery Esar Setyawan Bekti Wibowo Setyo Purwono Setyo Purwono Siswanto Siswanto Sitti Rahmah Umniyati Sitti Rahmah Umniyati Sofia Mubarika Haryana Srikanth Karnati Süleyman Ergün Tety Hartatik Titien Budhiaty Tri Baskoro Tunggul Satoto Widya Wasityastuti Woro Rukmi Pratiwi Wuryanto Hadinugroho Yahiddin Selian Yasmeen Kwok Yudy Tjahjono Yufita Ratnasari Wilianto Yuliani, Fara Silvia Yuniyanti, Mia Munawaroh Zafrullah, Umar Farooq