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Auliyah, Nabila Dhiyaul
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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Journal : Journal of Pharmaceutical and Sciences.

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Analisis kadar amilosa pada pati jagung manis (Zea Mays L. Var. Saccharata) secara spektrofotometri UV-Vis Fadjria, Neri; Arfiandi, Arfiandi; Auliyah, Nabila Dhiyaul
Journal of Pharmaceutical and Sciences JPS Volume 7 Nomor 2 (2024)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v7i2.494

Abstract

Sweet Corn Starch (Zea mays L. var. saccharata) is composed of two fractions: amylose and amylopectin. The determination of amylose content in Sweet Corn Starch is carried out using the spectrophotometric method. This method employs a 2% iodine solution as a reagent, which reacts with starch to generate a coloured solution that can be measured using a spectrophotometer. The amylose content is quantified through visible spectrophotometry, resulting in a regression equation of y ̂ = 0.006 + 25.2x, with a coefficient of determination (R2) = 0.998 and a correlation coefficient (r) = 0.9989. The amylose content in Sweet Corn Starch is determined to be 28.65%, whereas the amylopectin content is found to be 71.35%. Furthermore, the analysis provides additional parameters: Standard Deviation (SD) = ± 0.0044, Detection Limit (BD) = 0.0005 mg/ml, and Quantity Limit (BK) = 0.0018 mg/ml. These parameters offer valuable insights into the quality and properties of the sweet corn starch sample.
Co-Authors Arfiandi, Arfiandi Fadjria, Neri