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Annales Bogorienses
Published by Badan Riset dan Inovasi Nasional
ISSN : 05178452     EISSN : 24077518     DOI : https://doi.org/10.55981/ann.bogor
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Annales Bogorienses aims to disseminate high-quality scientific research in the field of life sciences, with a strong emphasis on advancing knowledge and applications in biotechnology, molecular biology, biochemistry, bioinformatics, and bioengineering. The journal serves as a platform for researchers, academicians, and practitioners to share original findings, innovative methodologies, and critical reviews that contribute to scientific progress and sustainable development. The journal covers research in biotechnology, molecular biology, biochemistry, bioinformatics, and bioengineering. It publishes original research articles, reviews, and short communications, and is committed to rigorous peer review and open access for the widest possible dissemination of scientific knowledge.
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Articles 5 Documents
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Search results for , issue "Vol. 11 No. 1 (2007): Annales Bogorienses" : 5 Documents clear
Agrobacterium-Mediated Transformation of Javanica Rice Plants With A Cry1b Gene Under The Control of Wound-Inducible Gene Promoter Estiati, Amy; Rachmawati, Syamsidah; Astuti, Dwi; Loedin, Inez Hortenza Slamet
Annales Bogorienses Vol. 11 No. 1 (2007): Annales Bogorienses
Publisher : BRIN

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Abstract

A cry1B synthetic gene of Bacillus thuringiensis has been used for the transformation of the javanica rice plants cv. Rojolele to confer resistance to an important pest yellow stem borer (Scirpophaga incertulas). Embryogenic callus were co-cultivated with the EHA105 strain of Agrobacterium tumefaciens harbouring binary vector pCAMBIA I301 containing cry1B gene under the control of wound inducible gene promoter (mpi), hygromycin resistance gene (hpt) as a selectable marker and intron-containing b-glucuronidase (gus intron) gene as a reporter gene driven by CaMV35S promoter. Previously. our histochemical assay and PCR analysis had proved the integration of cry1B gene into the genome of rice plants at first generation. However, the existence of the gene should remain stable throughout generation. In this study, the presence of the cry1B transgene in rice transgenic plants at second generation was confirmed by Polymerase Chain Reaction (PCR). Insertion of the cry1B gene in the genome of PCR positive plants was verified by Southern blot analysis and showed that integration of cry1B into the genomic DNA of javanica rice plants cv. Rojolele. An effective resistance of transgenic plants against stem borer was verified in bioassays.
Motif II of Japanese Encephalitis Virus NS3 Protein is Not Essential for RNA Binding Activity Utama, Andi; Shimidzu, Hiroyuki
Annales Bogorienses Vol. 11 No. 1 (2007): Annales Bogorienses
Publisher : BRIN

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Abstract

The role of motif II (DEAH; Asp285-Glu286-Ala287-His288) of Japanese encephalitis virus (JEV) NS3 protein on RNA binding activity was studied. A point mutation was introduced to the motif and the RNA binding activity of each mutant protein was analyzed. Truncated form of each protein with a His-tag was expressed m Escherichia coli BL21(DE3)pLysS and purified by metal affinity resin. Asp-285 and Glu-286 was respectively substituted with Ala. Ala-287 was replaced by Cys, Gly, or Ser. His-288 was mutated to other 19 amino acids. In total, 24 mutant proteins were produced and analyzed. As results, all mutants showed quite similar RNA binding activity indicating that motif II of JEV NS3 is not related to RNA binding activity. The same finding was reported for hepatitis C viruc (HVC) NS3 protein, suggesting lhe similar structure of NS3 protein in the flavivirus.
Identification and Activity of The Retrotranposon Tos17 in Indonesian Javanica Rice CV. Rojolele and Japonica Rice CV. Gajahmungkur Nugroho, Satya; Loedin, Inez Hortenza Slamet; Ouwerkerk, Pieter B. F.
Annales Bogorienses Vol. 11 No. 1 (2007): Annales Bogorienses
Publisher : BRIN

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Abstract

Retrotransposons are mobile genetic elements that transpose via an RNA intermediate that is revers transcribed before integration into a new location within the host genome. They are ubiquitous in eukaryotic organisms and constitute a major portion of the nuclear genome (often more than half of the total DNA) in plants. Tos17 is a rice endogenous retrotransposon that has been studied thoroughly. Tos17 has been shown to be an efficient insertional mutagen and saturation mutagenesis tool for gene tagging and functional genomics in Japonica rice cv nipponbare. In Javanica rice, however, the presence and activity of Tos17 has not been described thus far. while in some Indica rice Tos17 has been found to be inactive. Javanica rice, also known as tropical Japonica rice, has many cultivars which may serve as potential genetic resources of great interest for breeding programmes. Here, the presence and activities of retrotransposon Tos17 in Javanica rice cv Rojolele was described and compared to those of Japonica rice and Japonica rice cv Gajahmungkur. We identified five and three copies of Tos17 in Rojolele and Gajahmungkur, respectively, with different activities.
Present Status and Challenges on Agricultural Microbe Bio-Prospecting in Indonesia Sukara, Endang
Annales Bogorienses Vol. 11 No. 1 (2007): Annales Bogorienses
Publisher : BRIN

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Abstract

Food security is one of the essential needs which can never be ignored by any society, including in particular Indonesia, as the world population is expected to rise to around 10 billion marked by 2025. The problem is that the agriculture in developed and developing countries that follow the path of developed countries experiences create a range of serious environmental degradation. An intensive agricultural production through the use of pesticide and herbicides, forest clearing and continued use of land resources for the agricultural purposes are not sustainable practices. In consequences, degradation of ecosystem, oil erosion, and low productivity of land, poor water quality and lost of biological diversity (including microbial and genetic resources) occurred at alarming state. Meanwhile, the efforts in addressing the above situation is traditionally focused on plant phen types (breeding. plant genetic engineering, etc.) but the important role of microbial communities that interact with plants to influence plant health and productivity has been largely ignored. The interaction of microbes with plants and its relevant to soil fertility and productivity, the biocontrol properties of microbes and challenges toward sustainable agriculture is highlighted.
Construction of Binary Vector With Wound Inducible Promoter for HbsAg Expression: Development of Plant-Based Edible Hepatitis B Vaccine from Indonesian Isolate Suhandono, Sony; Giri-Rachman, Ernawati A.; Zainuddin, Ima M.; Utari, Putri Dwi; Supraba, Apsari; Estiati, Amy
Annales Bogorienses Vol. 11 No. 1 (2007): Annales Bogorienses
Publisher : BRIN

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Abstract

Hepatitis B is a serious infectious disease in the third world countries including Indonesia. Vaccination is the most effective way to prevent the spread of the disease; therefore the demand for HBV vaccine is high. In order to produce more vaccine at lower cost, transgenic plant can be chosen to express the vaccine with the above criteria. Several researches were successfully producing transgenic plants expressing HBsAg that formed virus-like particles and induced immune response in human. However, HBsAg expression in transgenic plant needs to be improved especially on gene expression control system. Here, we describe the construction of HBsAg structural gene under the control of wound inducible promoter, MeEF1 promoter from Manihot esculenta Crantz. The HBsAg gene was amplified using PCR from HBV genome isolated from an Indonesian patient. The gene was subsequently fused with VSPaS signal peptide, which targeted the reticulum endoplasm of plant cell. The construct was cloned into binary expression vector for Agrobacterium plant transformation in near future.

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