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Annales Bogorienses
Published by Badan Riset dan Inovasi Nasional
ISSN : 05178452     EISSN : 24077518     DOI : https://doi.org/10.55981/ann.bogor
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Annales Bogorienses aims to disseminate high-quality scientific research in the field of life sciences, with a strong emphasis on advancing knowledge and applications in biotechnology, molecular biology, biochemistry, bioinformatics, and bioengineering. The journal serves as a platform for researchers, academicians, and practitioners to share original findings, innovative methodologies, and critical reviews that contribute to scientific progress and sustainable development. The journal covers research in biotechnology, molecular biology, biochemistry, bioinformatics, and bioengineering. It publishes original research articles, reviews, and short communications, and is committed to rigorous peer review and open access for the widest possible dissemination of scientific knowledge.
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Articles 5 Documents
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Search results for , issue "Vol. 13 No. 1 (2009): Annales Bogorienses" : 5 Documents clear
Effect of Culture Conditions on Phytase Production by Aspergillus ficuum in Solid-state Fermentation Using Rice Bran as Substrate Kusharyoto, Wien; Sari, Martha; Ridwanuloh, Asep Muhamad
Annales Bogorienses Vol. 13 No. 1 (2009): Annales Bogorienses
Publisher : BRIN

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Phytic acid is an antinutritional factor that forms 1–2% of most of the seeds and their co-products representing more than 60% of their total phosphorus. Monogastric and agastric animals are unable to utilize phytate phosphorus either due to lack of or insufficient amount of phytate degrading enzymes. Phytases (myo-inositol hexakisphosphate-phosphohydrolase) are a special class of phosphatases that catalyze the hydrolysis of phytic acid in a stepwise manner to lower inositol phosphates, myo-inositol and inorganic phosphate. Phytases are found naturally in plants and microorganisms and a sizeable number of phytases have been purified and characterized from various fungi, yeasts, and bacteria. The present investigation involves studies on the effect of moisture content, pH value and different media ingredients such as carbon, nitrogen, and surfactants on the production of phytase by the fungus Aspergillus ficuum DSM 932 in solid-state fermentation (SSF) using rice bran as substrate. The production of phytase by SSF was favored, when the fungus was grown at a moisture content of 60% and pH 7.0, resulted in a phytase activity of 5.2 units/g dry substrate. There was a 20% increase in phytase yield in the presence of sucrose in SSF medium, while glucose and fructose were not effective in enhancing the phytase activity when used individually. Yeast extract was found to be a favorable nitrogen source for phytase production by SSF, which resulted in a 20% increase in phytase activity. There was no significant effect in increasing phytase production with the use of either soy peptone or tryptic soy as nitrogen source. Approximately 30% inhibition in phytase activity was shown in the presence of the surfactant Tween-80 or Triton X-100 in the SSF. By supplementing rice bran with sucrose and yeast extract, and performing the SSF in tray bioreactors, a phytase activity of 6.76 units/g dry substrate could be obtained.
Agrobacterium-mediated Transformation of Banana Musa acuminata AA cv "“Mas Lampung” with hpt Gene Using Sterile Corm as Target Tissue Estiati, Amy; Nena, Ade; Witjaksono, Witjaksono
Annales Bogorienses Vol. 13 No. 1 (2009): Annales Bogorienses
Publisher : BRIN

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The protocol for Agrobacterium-mediated transformation of local banana plants cv “Mas Lampung” (AA) has been established. A selectable marker gene (hpt) has been used to study the transformation using in vitro corm slices as target tissues. Banana in vitro corm slices were co-cultivated with the EHA105 strain of Agrobacterium tumefaciens harbouring binary vector pCAMBIA 1301 containing hygromycin resistance gene (hpt) as a selectable marker and intron-containing β-Glucuronidase (gus-intron) gene as a reporter gene driven by CaMV 35S promoter. Polymerase Chain Reaction (PCR) were used to examine the existence of hpt gene in plants resulted from the transformation. Using primer pairs specific for hpt gene, our PCR analysis on leaves showed the presence of the hpt transgene in banana transgenic plants at first generation (T0) of transformation. To prove the existence of hpt gene in the fruits of transgenic banana plants, PCR analysis were also carried out. The data showed that the hpt gene could be amplified from banana fruits of tested samples. These result demonstrates that the Agrobacterium-mediated transformation method used in this experiment has been successful to transfer gene into banana plants. Thus, the transformation method reported here could be used as a standard protocol to transfer another useful genes into local banana plants cv. “Mas Lampung”. Furthermore, the presence of transgene in fruits of banana transgenic plants is important achievement especially for transgene that is expected to be expressed in the fruit including to introduce vaccine genes into banana fruits for edible vaccine.
Propagation of Sukun (Artocarpus altilis (Parkinson) Fosberg) Through In Vitro Shoot Proliferation Imelda, Maria; Wulansari, Aida; Sari, Laela
Annales Bogorienses Vol. 13 No. 1 (2009): Annales Bogorienses
Publisher : BRIN

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Sukun (Artocarpus altilis (Parkinson) Fosberg) of the Moraceaae is a big tree which can grow to 15-20 m in height and native to the Asia-Pasific region. Beside its delicious fruit, sukun is also known as a traditional herbal medicinal plant in the region, including Indonesia. Nearly all parts of the plant, such as roots, stems and leaves are believed by local communities to be capable of curing liver disease, hypertension, cardiac arrest, toothache, renal problem and even skin itchiness. The collaborative research between LIPI and PR China, on developing herbal medicines indicated that sukun has a great potential for treating cardiovascular disease. However, the availability of raw materials still poses a big constraint for the industry of herbal medicines. Generally, sukun is propagated by root or stem cuttings, since in Indonesia sukun does not produce any seeds. However such method only produces limited planting materials. In general tissue culture propagated plants have many advantages, namely being clonal, free from pest and diseases, more uniform, and allowing a high rate of plant multiplication. Therefore, the technique for sukun propagation has been developed by the LIPI Research Centre for Biotechnology. In this research the effects of 1-5 mg/l benzyl amino purine (BAP) and 20-40 mg/l adenine sulphate (AS)on shoot bud proliferation were investigated using lateral shoot buds on a modified Murashige and Skoog (MS) medium with addition of 150 ml/l coconut water (CW). Shoots were rooted on MS medium without plant growth regulators (PGRs). The results showed that the best medium for in vitro shoot proliferation was a modified MS medium containing 2 mg/l BAP, 40 mg/l AS and 150 ml/l CW. The best medium for rooting is MS medium containing 1 mg indole butyric acid (IBA), producing roots within 3 weeks.
Identification of Drought Tolerant Related Insertional Mutant Lines Using PEG 6000 Nugroho, Satya; Windiastri, Vincentia Esti; Widyajayanti, Dwi; Pantouw, Carla Frieda
Annales Bogorienses Vol. 13 No. 1 (2009): Annales Bogorienses
Publisher : BRIN

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Drought is one of the most important abiotic stresses in rice (Oryza sativa) productivity. The development of drought tolerant cultivars are therefore highly desireable. We have developed insertional mutant based on the Japonica rice cv Nipponbare rice by transposons Ac/Ds insertions containing activation-tag and gene trap. Screening of the mutant population for drought tolerant related phenotypes is of our priority. The screening protocol based on PEG 6000 has been developed and was being used to screen 70 mutant lines tocharacterize their responds to the treatment based on different parameters (number of leaf, total weight, plant height, root length and number of germinating seeds). These characters were used to score the Degradation Index and Vigour Index. Results showed varyingresponds of the lines to the osmotic pressure. Some lines showing a good performance indicated by lower Degradation Index and higher Vigour Index have been identified. Some inconsistencies in the performances scored by both indices were thought to be due to seed quality.
Gene Flow from Genetically Modified Rice to Their Weedy and Wild Relatives and Its Environmental Consequences Estiati, Amy; Nugroho, Satya
Annales Bogorienses Vol. 13 No. 1 (2009): Annales Bogorienses
Publisher : BRIN

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Abstract

Rice is one of the most important food crops in the world. Nearly half of the world population consume rice as their staple food (FAO, 2004). With the increasing of world population, the need to provide more food supplies including rice is obvious. Biotechnology is expected to play major roles in the improvement of crop productivity and quality. Unlike transgenic maize, eventhough many research have been done to improve rice performance by genetic engineering, transgenic rice has not been released or commercialized. Among those research to improve rice performance are the attempt to introduce biotic and abiotic stress tolerant traits, herbicide tolerant trait and beta-carotin biosynthetic pathway. Currently, several genetically modified rice cultivars have been and being tested on limited field trials. Undoubtedly, biotechnology will benefit agriculture and thus providing enough food source to keep up with the ever-increasing needs in the future. However, the release and utilization of such technology in agriculture is still arising concerns about the impact to the environment. Therefore the possibility of the transgene escape to the environment needs to be analyzed. For example, whether the application of such technology can create superweed that resulted in the environment problems. On the other hand, cultivated rice may acquire genes for weediness from pollen-mediated gene flow of weedy or wild rice occurring inside or near cultivated rice fields, which leads to persistence and invasiveness of the cultivated rice, although the chance is low. This article discuss the possibility of the occurrence gene flow from the application of genetically modified rice to surrounding rice plants including its wild relatives and weed, and the requirements or precautions needed to be done to prevent gene flow. 

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