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Tika Hairani
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Gedung Administrasi, Kawasan Sains Teknologi Dr. (H.C) Ir. H. Soekarno, Jl. Raya Bogor KM. 46, Cibinong 16911
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Annales Bogorienses
Published by Badan Riset dan Inovasi Nasional
ISSN : 05178452     EISSN : 24077518     DOI : https://doi.org/10.55981/ann.bogor
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Annales Bogorienses aims to disseminate high-quality scientific research in the field of life sciences, with a strong emphasis on advancing knowledge and applications in biotechnology, molecular biology, biochemistry, bioinformatics, and bioengineering. The journal serves as a platform for researchers, academicians, and practitioners to share original findings, innovative methodologies, and critical reviews that contribute to scientific progress and sustainable development. The journal covers research in biotechnology, molecular biology, biochemistry, bioinformatics, and bioengineering. It publishes original research articles, reviews, and short communications, and is committed to rigorous peer review and open access for the widest possible dissemination of scientific knowledge.
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Search results for , issue "Vol. 17 No. 2 (2013): Annales Bogorienses" : 6 Documents clear
EDITOR'S PREFACE Lisdiyanti, Puspita
Annales Bogorienses Vol. 17 No. 2 (2013): Annales Bogorienses
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Enhancing the Immunogenicity of Subunit Vaccines by Utilisation of Particulate Vaccine Delivery Systems Prasetyoputri, Anggia; Kusharyoto, Wien
Annales Bogorienses Vol. 17 No. 2 (2013): Annales Bogorienses
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Control and eradication of a number of infectious diseases are primarily attributed to effective vaccination programs. A concerted effort is still imperative to develop novel vaccines and improve the immunogenicity of existing ones with regards to efficacy, immunogenicity and safety. Rational design of vaccines using subunit vaccines is a potentially safer alternative to conventional vaccines, yet they are poorly immunogenic without additional adjuvant. Using antigen carriers to enhance their immunogenicity in the forms of adsorption or encapsulation with a delivery system has been widely investigated as an alternative to currently available adjuvants. This review aims to elaborate on the existing nanotechnology being used to develop more immunogenic subunit vaccines, with focus on particulate delivery systems for development of prophylactic vaccine candidates. 
Insect Bioassay in Biosafety Containment to Select Transgenic Rice (Oryza sativa L.) Harboring Cry1B Gene Resistant to Yellow Stem Borer (Scrirpophaga incertulas Walk.) Estiati, Amy; Nurhasanah, Ade Nena; Nugroho, Satya
Annales Bogorienses Vol. 17 No. 2 (2013): Annales Bogorienses
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Development of rice varieties resistant to yellow stem borer (YSB) is very crucial. Agrobacterium-mediated transformation of cry1B gene under wound inducible gene promoter mpi (maize proteinase inhibitor) into a local rice variety Rojolele had been conducted. PCR analysis proved that cry1B gene had been integrated into plant genome of 3R25 and 3R5 rice lines. Segregation analysis using PCR for cry1B gene of the two putative transgenic rice lines at third (T2), fourth (T3), fifth (T4), and sixth (T5) generations of 3R25 and 3R5 lines proved that 3R25.7.27, 3R25.7.13.8.2, 3R25.7.13.8.6, 3R25.7.13.8.8, 3R5.26.2, and 3R5.26.5 are homozygous lines for cry1B gene. Insect bioassay on three randomly picked homozygous transgenic rice lines to study the efficacy of cry1B gene toward YSB was conducted in biosafety containment by infestingYSB larvae at first instar into 3R25.7.27, 3R25.7.13.8.6, and 3R5.26.5 transgenic rice lines, using non--transgenic Rojolele, IR64 and IR74 as susceptible controls. The results showed that the percentages of deadhearts symptoms of 3R25.7.27, 3R5.26.2, and 3R25.7.13.8.6 rice lines were lower than those of the susceptible control lines with scores of 0.1 and 0, respectively. While the scores of all three susceptible control plants were 9. The results proved that lines 3R25.7.27, 3R25.7.13.8.6, and 3R5.26.2 were categorized as resistant lines while the non-transgenic Rojolele, IR64, and IR74 were categorized as susceptible lines. The results also showed that the cry1B gene was expressed and produced insecticidal protein CRY1B which were active against YSB to protect rice plant toward YSB infestation.
Detection of Keto Synthase (KS) Gene Domain in Sponges and Bacterial Sponges Atikana, Akhirta; Naim, Mohamad Azrul; Sipkema, Detmer
Annales Bogorienses Vol. 17 No. 2 (2013): Annales Bogorienses
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Sponges are sources of various useful natural products. The natural compounds derived from sponges are mostly complex polyketide. The polyketide synthase genes (PKS) is responsible for the biosynthesis of complex polyketides. During this research, we detected ketosynthase (KS) gene domain from 10 species of marine sponges, collected from Mediterranean Sea (Aplysina aerophoba, Petrocia ficiformis, Axinella damicornis, Axinella veronicas, Crambe crambe) and North Sea (Halichondria panicea, Haliclona xena, Haliclona oculata, Suberites massa, Ephydatia fluviatilis). We also detected KS gene domain from 12 bacterial sponge isolated from H. panicea. We used a PCR-based approach to detect the ketosynthase (KS) gene domain of Polyketosynthase (PKS) genes. Our result showed that the sponges and the bacterial sponges are mostly KS positive (700 bp). The presence of the PKS gene indicates the ability of the sponges to produce bioactive compound and potential natural product. The presence of the KS domain in both of the sponges and the bacterial sponges might also indicate that the bacterial sponges involved in the biosyntesis of secondary metabolite of the sponges.
Potency of Endophyte Bacterium Isolated from Shorea selanica on Producing IAA Hormone and Supporting the Growth of Soybean Widowati, Tiwit; Nuriyanah, Nuriyanah; Sukiman, Harmastini
Annales Bogorienses Vol. 17 No. 2 (2013): Annales Bogorienses
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Growth of soybean plants was supported by the presence of nitrogen fixing bacteria. Besides nitrogen, other elements such as phosphate, potassium and growth hormones are also required. Endophytic bacteria associated with Shorea selanica were isolated and tested for their ability to produce indole acetic acid (IAA) hormone and exhibit stress tolerance. Colorimetric analysis showed that isolate SSBt2 produced the highest IAA (43.01 μg/ml) in culture supplemented with L-tryptophan. Isolate SSBt2 grew well in the some stress tests, except on heat and oxidative stress. The highest IAA producing strain was selected for determining its capability and compatibility to support the growth of soybean plants in glass house experiment. The results indicated that the endophytic bacteria isolated from S. selanica are compatible to support the growth of soybean. SSBt2 was identified as Enterobacter hormaechei based on 16S rRNA gene analysis.
Isolation and Screening of Surfactant-producing Bacteria from Indonesian Marine Environments and Its Application on Bioremediation Susilaningsih, Dwi; Okazaki, Fumiyoshi; Yopi, Yopi; Widyastuti, Yantyati; Harayama, Shigeaki
Annales Bogorienses Vol. 17 No. 2 (2013): Annales Bogorienses
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Isolation and screening have been undertaken on oil-degrading microbes from Indonesian marine environments. During screening process it has been found many bacterial isolates capable of degrading crude oil. Hence, study has been focused on the biodiversity of biosurfactant-producing bacterial species in Indonesian marine environment and its function for remedial the pollutant in marine and soil areas. A total of 103 out of 463 isolates showed positive surfactant-degrading properties. By means of partial 16S rRNA gene analyses, it has been found that the majority of taxa are related to Alcanivorax, Pseudomonas, Bacillus, Bortetela, Brucella, Acenitobacter, Staphia, Lysobacter, and Talasosophira. Biosurfactant properties assay showed that they were capable of lowering the surface and interfacial water tension from 74 mN/m to 40-65 mN/m and from 24 mN/m to 6-10 mN/m, respectively. In addition, most of the surfactants were capable of emulsifying hydrocarbon (crude oil) of 0.01 to 0.15 units, comparable to 0.08 units of synthetic surfactant (20% Tween). Further observation showed that the majority of the surfactants were able to degrade a long chain of alkane, but not branched alkane, with a recovering rate of 20-80%. The application of the surfactant towards oil polluted model beach was done in laboratory scale and showing the surfactant obtained from microbial broth cultures capable for recovering the oil pollutant significantly, compared to the control (without addition microbial broth).

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