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Annales Bogorienses
Published by Badan Riset dan Inovasi Nasional
ISSN : 05178452     EISSN : 24077518     DOI : https://doi.org/10.55981/ann.bogor
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Annales Bogorienses aims to disseminate high-quality scientific research in the field of life sciences, with a strong emphasis on advancing knowledge and applications in biotechnology, molecular biology, biochemistry, bioinformatics, and bioengineering. The journal serves as a platform for researchers, academicians, and practitioners to share original findings, innovative methodologies, and critical reviews that contribute to scientific progress and sustainable development. The journal covers research in biotechnology, molecular biology, biochemistry, bioinformatics, and bioengineering. It publishes original research articles, reviews, and short communications, and is committed to rigorous peer review and open access for the widest possible dissemination of scientific knowledge.
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Search results for , issue "Vol. 19 No. 2 (2015): Annales Bogorienses" : 8 Documents clear
EDITOR'S PREFACE Lisdiyanti, Puspita
Annales Bogorienses Vol. 19 No. 2 (2015): Annales Bogorienses
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Roferon-A: A Biologic Product of Human Interferon Alpha 2a Wardiana, Andri; Ningrum, Ratih Asmana
Annales Bogorienses Vol. 19 No. 2 (2015): Annales Bogorienses
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Human interferon alpha 2a (hIFNα2a) is a cytokine regulating immune system that has been used in hepatitis and cancer treatments. It has wide biological potency covering antiviral, antiproliferative and immunomodulative activities. This mini review discusses Roferon-A as a prominent commercial product of recombinant hIFNα2a which is produced in bacterial system, Escherichia coli, as therapeutic protein for several diseases, such as chronic viral Hepatitis B, Hepatitis C, melanoma, hairy cell leukemia and renal cell carcinoma. The discussion focuses on the development process with regard to its manufacturing, preclinical and clinical studies, as well as therapeutic efficacy. In addition, we also discuss biosimilar development of hIFNα2a and its potential future developments in the context of enhancing pharmacokinetic profiles.
Isolation, Identification, and Evaluation of Antimicrobial Activity of Active Compound Produced by Marine Actinomycetes isolate A32 Sunaryanto, Rofiq; Marwanta, Edy
Annales Bogorienses Vol. 19 No. 2 (2015): Annales Bogorienses
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The continuation of new antibiotics exploration becomes an important research program in the world for pharmaceutical and agricultural applications. Marine filamentous bacteria such as actinomycetes have been widely used as an important biological tool to generate a variety of new secondary metabolites, such as antibiotic. The aim of this study was to obtain identified active compound and determine its antimicrobial activity. Isolation, identification, and antimicrobial activity assay of active compound produced by marine actinomycetes isolate A32 had been conducted. Production of active compound using isolate actinomycetes A32 was conducted involving glucose, yeast, peptone medium. The fermentation was carried out at 30 ºC for 5 days. The broth of supernatant was extracted using ethyl acetate. Purification of active compound used chromatography column and eluted stepwise with the chloroform and methanol solvents. Antimicrobial activity was monitored using agar disc diffusion, and microbial test was conducted by analyzing the samples diameter of clear zone towards Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 66923, and Candida albican BIOMCC 00122. Results of isolation and purification of active compound produced by actinomycetes isolate A32 show that this compound has a molecular weight of 503.1 g/mol with molecular formula C26H37N3O7. Furthermore, this compound was suspected as Madumycin II after analysis of spectrum using 1HNMR and COSY. The antimicrobial activity assay confirms that this active compound inhibited the growth of Staphylococcus aureus ATCC 25923 and Candida albican BIOMCC 00122.
Antioxidant Capacities of Holothuria Sea Cucumbers Murniasih, Tutik; Putra, Masteria Yunovilsa; Pangestuti, Ratih
Annales Bogorienses Vol. 19 No. 2 (2015): Annales Bogorienses
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Sea cucumbers are a potential source of biologically active metabolites that are widely used for nutraceutical, pharmaceutical and cosmeceutical products. In this study we investigated the antioxidant activity of several extracts of sea cucumbers, those are Holothuria scabra, Holothuria atra, Holothuria leucospilota, and Holothuria excellens collected from Lombok Island. The selected sea cucumber was then separated to get the active fractions and identified for profiling the secondary metabolites. Phytochemical screening reveals the presence of flavonoid, terpenoids, phenol, saponin, alkaloid, anthraquinone, and glycoside in the extracts of Holothuria sea cucumbers. Radical scavenging effects on the DPPH (1,1-diphenyl-2-picryl-hydrazyl) model were evaluated. The previous work reported that the crude extract of H. atra showed the highest potential of antioxidant capacity. H. atra extract was separated by column chromatography on silica gel and eluted with a gradient system of increasing polarity. Fraction 7 of H. atra extract showed significantly inhibited radical scavenging activity (35.3487 %) at concentration 1 mg/ml. The result of Gas Chromatography-Mass Spectrometry analysis of H. atra fraction 2 showed that it has several active antioxidant compounds which are ethyl cetylate (3), hexadecyl-oxirane (5), 3-chloro-4-hydroxybenzoic acid (6), andoleyl alcohol, trifluoroacetate (9). Moreover, the active fraction 7 indicated several compounds, those are cycloicosane (17), pentadecyl trichloroacetate (18), 14b-pregnane (21) and cis-4-yano-2-(2-hydroxycyclohexyl) pyridine. However, these are preliminary findings and further studies are needed to be focus on the purifications of unknown compounds and formulation for nutraceuticals.
Improvement of Endoglucanase Activity in Penicillium oxalicum ID10-T065 Mutated by Ultra Violet Irradiation and Ethidium Bromide Caniago, Asnany; Mangunwidjaja, Wibowo; Nuswantara, Sukma; Lisdiyanti, Puspita
Annales Bogorienses Vol. 19 No. 2 (2015): Annales Bogorienses
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Penicillium sp. is known as a filamentous fungus that produces complete cellulase. This study aims to improve endoglucanase activity of Penicillium oxalicum ID010-T065 by mutation with ultra violet irradiation (with dose of 0.1 J/cm2, 15 cm), ethidium bromide (10 μg/mL, 1 hour) and combination of both mutagens. The endoglucanase activity of all mutants was higher than that of the wild type (1.03 U/mL). Mutant UVEB-42 exposed to combine mutation showed the highest endoglucanase activity (2.76 U/mL) with a 2.70 fold increase. Mutant EB-45 (1.83 U/mL) exposed to ethidium bromide solution showed a 1.8 fold increase. Mutant UV-13 (1.72 U/mL) exposed to UV irradiation for 3 minutes showed a 1.7 fold increase. All mutants have optimum endoglucanase activity at 50 °C. Mutant UVEB-53 showed the highest thermostability by retaining 86 % of endoglucanase activity at 90 °C. The gene analysis of the endoglucanase I gene (eg1) showed 3 bases mutation in mutant UV-13 and UVEB-53 that changed proline to serine. Mutant EB-45 showed 4 mutated bases that changed valine to glysine and proline to serine. Two bases mutation in Mutant UVEB-53 changed proline to serine. Bases mutation which is occured in eg1 gene could influence the enhance of endoglucanase activity in mutant.
Production of Phytase, Amylase and Cellulase by Aspergillus niger, Neurospora crassa, and Rhizophus oryzae on Sargassum and Rice Bran Under Solid State Fermentation Kanti, Atit
Annales Bogorienses Vol. 19 No. 2 (2015): Annales Bogorienses
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The objective of study was to produce amylase, cellulase and phytase on sargassum and rice bran on solid state fermentation using Aspergillus niger, Neurospora crassa, and Rhizophus oryzae. Media for solid state fermentations composed of dried sargassum and rice bran. The effect of particle of sargassum, initial moisture content on phytase, amilase, and cellulase were evaluated. Optimum enzyme activity of phytase, amylase and cellulase were obtained after 4 days fermentation at 30°C, and initial moisture content was adjusted to 60%. The optimum particle size of dried sargassum attaining the highest enzyme activity was 25 mesh. Best formula forenzymes production was at the ratio of 4:6 (w/w) of Sargassum spinosum (SS) and rice bran (RB) respectively. At this formula highest phytase activity was obtained by Aspergillus niger, cellulase by Rhizopus oryzae, and amylase by Neurospora crassa. Media composed of sargassum and rice bran can be used for phytase, amylase and cellulase production.
Isolation and Identification of Antiplasmodial Compound from Methanol Extract of Calophyllum bicolor P. F. Steven Abbas, Jamilah; Hanafi, Muhammad; Artanti, Nina; Sundowo, Andini; Minarti, Minarti; Asih, Puji Budi Setia; Syafrudin, Din
Annales Bogorienses Vol. 19 No. 2 (2015): Annales Bogorienses
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Calophyllum bicolor (Clusiacea) is a big tree from Indonesian rain forest in Palangkaraya, Central Kalimantan. Calophyllum or bintagor is one of many sources of natural bioactive compounds that can be used in the fields of health and pharmaceuticals. The aim of this research was to explore the antiplasmodial activity of methanol extract of Calophyllum bicolor P.F. Steven against Plasmodium falciparum. The methanol extract was purified by colomn chromatography system, hexane – ethyl acetate was used as solvent with increasing polarity. One pure compound was obtained and was elucidated based on the 1H-&13C-NMR and 2D-NMR, [COSY, HMBC and HMQC] data and the isolated compound was identified as xanthone. Methanol extract showed antiplasmodial activity growth inhibition against P. falciparum with IC50 5.2 ppm and the new 5-methoxy trapezifolixanthone compound have maximum inhibition at concentration 0.11 nMol.
Expression of No Affinity Tagged Recombinant Human Interferon Alpha-2a in Methylotrophic Yeast Pichia pastoris Herawati, Neng; Wardiana, Andri; Ningrum, Ratih Asmana
Annales Bogorienses Vol. 19 No. 2 (2015): Annales Bogorienses
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Recombinant human interferon alpha-2a (rhIFN-2a) has been widely used for clinical therapy as antiviral, anticancer as well as immunomodulator. In this study, the open reading frame (ORF) encoding synthetic hIFN-2a was constructed to be in framed with N-terminal alpha factor secretion system in methylotrophic yeast Pichia pastoris. This research aimed to construct, express and analyse the non-affinity tagged recombinant human interferon alpha-2a in the methylotrophic yeast P. pastoris. We used pPICZB plasmid for cloning and expression vector. The confirmed recombinant plasmid containing the correct DNA sequence of hIFN-2a was linearized by SacI restriction enzyme, then transformed into P. pastoris genome using electroporation. We screened two multi-copy recombinants in YPDS plates containing Zeocin™. Buffered complex medium containing 0.5 % methanol (BMMY) was used for protein expression for 48 hours in the culture condition. The recombinant protein was purified by blue sepharose affinity chromatography. Analyses of hIFN-2a protein by SDS-PAGE and Western blot confirmed that protein band in which was observed around 19.2 kDa, was recombinant hIFN-2a. The quantification of purified rhIFN-2a using colorimetric binichoninic assay (BCA) informed that the yield was 44 mg/L culture (OD600= 2-3). 

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