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Annales Bogorienses
Published by Badan Riset dan Inovasi Nasional
ISSN : 05178452     EISSN : 24077518     DOI : https://doi.org/10.55981/ann.bogor
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Annales Bogorienses aims to disseminate high-quality scientific research in the field of life sciences, with a strong emphasis on advancing knowledge and applications in biotechnology, molecular biology, biochemistry, bioinformatics, and bioengineering. The journal serves as a platform for researchers, academicians, and practitioners to share original findings, innovative methodologies, and critical reviews that contribute to scientific progress and sustainable development. The journal covers research in biotechnology, molecular biology, biochemistry, bioinformatics, and bioengineering. It publishes original research articles, reviews, and short communications, and is committed to rigorous peer review and open access for the widest possible dissemination of scientific knowledge.
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Articles 7 Documents
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Search results for , issue "Vol. 24 No. 2 (2020): Annales Bogorienses" : 7 Documents clear
Synthesis and Characterization of Alginate-Cellulose Xanthate Beads from Corn Stalk with NaCl As Porogen Rohmatullah, Wahyu Adhi Putra; Yulianti, Eny; Khoiroh, Lilik Miftahul; Mahmudah, Rif'atul
Annales Bogorienses Vol. 24 No. 2 (2020): Annales Bogorienses
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In this study, the synthesis of porous beads from corn stalks was carried out. The cellulose extracted from corn stalks was converted into cellulose xanthate and combined with alginate to form porous alginate-cellulose xanthate beads by the ionic gelation method. This study attempted to use sodium chloride (NaCl) as a porogen and zinc acetate as a crosslinker. Beads were characterized to determine the porosity, swelling properties, and functional groups using Fourier Transform Infra-Red (FTIR). The geometry of beads was analyzed by optical microscopy, and its surface morphology was analyzed by Scanning Electron Microscopy-Energy Dispersive X-ray (SEM-EDX). The results showed that corn stalks as agricultural waste material could be used to synthesize porous beads material. The swelling and porosity of beads increased with increasing concentration of NaCl. The presence of porogen has increased beads formation. The results demonstrate the crosslinks between zinc acetate and alginate were successfully characterized using FTIR. NaCl concentration of 9.5% resulted in the highest swelling properties (52.80%) and porosity (81.4%) of the beads.
Study on Endophytic Fungi Associated with Moringa oleifera Lam. Collected from Lombok Island, West Nusa Tenggara Ramadhani, Indriati; Rohadi, Hasnadiazahra; Yuliani, Yeni; Ilyas, Muhammad
Annales Bogorienses Vol. 24 No. 2 (2020): Annales Bogorienses
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An assemblage of endophytic fungi was isolated from Moringa oleifera Lam. collected from Lombok island, West Nusa Tenggara Province, Indonesia. Fungal endophytes were isolated using surface sterilization methods with slight modification. Forty-six selected endophytic fungal strains were isolated from the leaves, petioles, and stems of M. oleifera. The fungal strains identification through morphological observation and ITS rDNA-based molecular analysis showed that fungal endophytes were associated with host plants belonging to the taxa Alternaria, Cladosporium, Colletotrichum (Glomerella), Corynespora, Curvularia (Cochliobolus), Fusarium, Mucor, Ochrocladosporium, Phomopsis (Diaporthe), and Trametes. In this study, endophytic Phomopsis dominating the obtained strains, whereas 26% (12/46) strains were isolated from host plant samples.
The Purification of Rennin-Like Protease from Lactobacillus paracasei Isolated from Ettawa Goat Milk Putranto, Wendry Setiyadi; Mustopa, Apon Zaenal; Kusumawati, Arizah; Prastyowati, Anika
Annales Bogorienses Vol. 24 No. 2 (2020): Annales Bogorienses
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There is a protease produced by bateria that has characteristics similar to rennin from a calf. Rennin has the ability to clot casein in milk. Rennin-like protease (RLP) is produced by bacteria extracellularly. Lactic Acid Bacteria (LAB) have the potential to be developed for RLP production because they are safe and non-pathogenic bacteria. Rennin is needed in the process of milk coagulation to subsequently obtain a curd in the process of making cheese. In this study, the LAB isolated from Ettawa goat milk (isolate 2.12) which produced RLP was 99% identical to Lactobacillus paracasei based on 16S rRNA gene sequence analysis. The purification of the RLP L. paracasei 2.12 with 60% ammonium sulfate deposition, dialysis, and filtration gel chromatography Sephadex G-50 showed a single 38 kDa protein band with SMCA/SPA was 4.48 higher than that of the calf rennet with a ratio value of 1, therefore in this study, RLP L. paracasei 2.12 was developed as an alternative to renin in cheese making.
Inhibitory Potential of Black Seed (Nigella sativa L.) Bioactive Compounds Towards Main Protease of SARS-CoV-2: In Silico Study Ferdian, Pamungkas Rizki; Elfirta, Rizki Rabeca; Emilia, Qori; Ikhwani, Azra Zahrah Nadhirah
Annales Bogorienses Vol. 24 No. 2 (2020): Annales Bogorienses
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COVID-19, caused by SARS-CoV-2, has become a massive worldwide concern of the 21st century. One potential strategy to block the biochemical pathway of SARS-CoV-2 was by inhibiting the main protease (Mpro), which is a key enzyme on viral replication. Black seed (Nigella sativa L.) has a long history for its use as a traditional medicine. Therefore, we hypothesised that the black seed contains numerous active compounds that could potentially confer inhibitory activity against SARS-CoV-2 viral Mpro. In this study, 24 active compounds from black seed were tested. Compounds were screened using Lipinski's Rules and admetSAR, then docked to viral Mpro 7BQY by AutoDockTools-1.5.6 and AutoDock Vina using a site directed docking approach resulting in affinity energy (∆G) and binding data. We found that the most potential active compound of N. sativa is 3-[(4-Methylphenyl)sulfanyl]-1,3-diphenyl-1-propanone, since its affinity energy was -7.6 kCal.mol-1. Its similarity to N3 inhibitor based on Ligplot analysis and DS were 86.7% and 76.19%, respectively, and the occupancy on binding site based on Ligplot analysis and DS were 90.91% and 81.82%, respectively. These findings can be used as a starting point for further investigation using in vitro and in vivo studies.
Enhancement of Yield, Starch, and Amylose Content of Two Indonesian Cassava Genotypes by Producing Gamma Irradiated-Induced Mutants Sudarmonowati, Enny; Hartati, N. Sri; Supatmi, Supatmi
Annales Bogorienses Vol. 24 No. 2 (2020): Annales Bogorienses
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As an important carbohydrate-source food crop and raw materials of starch-based industries, accelerated cassava breeding has been extensively pursued through various procedures, among others, is mutant induction using gamma irradiation combined with molecular marker. Iding, a local genotype cassava has been compared with a national variety Adira 4 for its yield attributes following gamma irradiation at various dose levels ( 2, 20, 30, and 50 krad). The mutation rate was conducted based on morphological characteristics and identification using RAPD by employing random primer OPB-10. Evaluation was conducted on mutant lines producing high potential yield, high starch and amylose contents. The fourth generation of irradiated Adira 4 (2 and 30 krad) and Iding (2 krad) exhibited higher yield and amylose content as compared to their representative controls. The yield of 30 krad irradiated Adira 4 indicated by mean tuber weight per individual could reach more than twice of that of control and almost 1.5 times of that irradiated with 2 krad. The levels of amylose content of all irradiated ones, regardless of genotypes and gamma irradiation doses (2 and 30 krad) were higher than that of control. In most parameters, irradiated Adira 4 was superior to irradiated Iding.
Catechin Contents, Antioxidant, and Antibacterial Activities of Different Types of Indonesian Tea (Camellia sinensis) Nuryana, Isa; Ratnakomala, Shanti; Fahrurrozi, Fahrurrozi; Juanssilfero, Ario Betha; Andriani, Ade; Putra, Filemon Jalu Nusantara; Rezamela, Erdiansyah; Wulansari, Restu; Prawira-Atmaja, M. Iqbal; Lisdiyanti, Puspita
Annales Bogorienses Vol. 24 No. 2 (2020): Annales Bogorienses
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Tea is one of the most popular beverages in the world. Produced from Camellia sinensis leaves, tea has been studied to provide health benefits due to the content of important metabolites. This study aimed to investigate the catechin contents, antioxidant, and antibacterial activities of Indonesian tea varieties, namely green tea, black tea, and white tea. Tea infusion was prepared by extracting 1 g of each sample into 10 mL of distilled water and incubated at 80 °C for 60 min. The catechin and epigallocatechin gallate (EGCG) contents of tea extracts were determined using high-performance liquid chromatography (HPLC). Antioxidant activity was measured using the free radical method with 2,2-diphenyl-1-pycrylhidrazyl (DPPH), while antimicrobial activity was assesed using paper disc diffusion assay. The results indicated that green tea had the highest contents of catechin (646 ± 17.14 mg/L) and EGCG (997.8 ± 36.72 mg/L), and antioxidant activity with IC50 of 5.65 µg/mL. Furthermore, green tea and white tea extracts showed inhibitory activity against Gram-positive bacteria such as Micrococcus luteus, Bacillus subtilis, and Staphylococcus aureus whereas black tea had no activity against all bacterial strains tested. Generally, we concluded that white tea and green tea contributed to the higher content of catechins and exhibited strong antioxidant and antibacterial properties.
EDITOR'S PREFACE Lisdiyanti, Puspita
Annales Bogorienses Vol. 24 No. 2 (2020): Annales Bogorienses
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