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Annales Bogorienses
Published by Badan Riset dan Inovasi Nasional
ISSN : 05178452     EISSN : 24077518     DOI : https://doi.org/10.55981/ann.bogor
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Annales Bogorienses aims to disseminate high-quality scientific research in the field of life sciences, with a strong emphasis on advancing knowledge and applications in biotechnology, molecular biology, biochemistry, bioinformatics, and bioengineering. The journal serves as a platform for researchers, academicians, and practitioners to share original findings, innovative methodologies, and critical reviews that contribute to scientific progress and sustainable development. The journal covers research in biotechnology, molecular biology, biochemistry, bioinformatics, and bioengineering. It publishes original research articles, reviews, and short communications, and is committed to rigorous peer review and open access for the widest possible dissemination of scientific knowledge.
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Articles 5 Documents
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Search results for , issue "Vol. 9 No. 1 (2004): Annales Bogorienses" : 5 Documents clear
EDITOR'S PREFACE Lisdiyanti, Puspita
Annales Bogorienses Vol. 9 No. 1 (2004): Annales Bogorienses
Publisher : BRIN

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Abstract

The editors would like to announce that, in the years of 2002 and 2003, The Research Centre for Biotechnology The Indonesian Institute of Sciences (LIPI) did not publish Annales Bogorienses New Series due to some management problems. The last issue waspublished with Volume 8, Number 2, 2001. Under new editors, in this year of 2004, continuing the previous volume, this journal is published again starting with Volume 9, Number 1. In this ninth edition of Annales Bogorienses, we would also like to introduce anew look of this journal to our readers. We will I continue to publish this journal periodically twice a year (in June and December).Many thanks to all authors for their efforts, attentions, and time throughout the editing process that make this current edition become possible. Tolerance of our editorial judgments is much appreciated. We encourage our readers to submit original papers on topics of interest to life sciences researchers. June, 2004M. Ahkam SubrotoChief Editor
Sensitivity Improvement of A Direct Competitive Elisa for Atrazine by Exploiting Low Cross-Reactivity of An Atrazine-Specific Recombinant Antibody Fab-Fragment Kusharyoto, Wien; Schmid, Rolf D.
Annales Bogorienses Vol. 9 No. 1 (2004): Annales Bogorienses
Publisher : BRIN

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Abstract

The hapten-binding site of the antibody Fab-fragment K411B specific towards the herbicide atrazine (2-chloro-4-(ethylamino)-6-(isopropyJamino)-1,3,5-triazine) was modified by means of structural modeling and site-directed mutagenesis. A triple mutant (GlnL89Glu/ValH37Ile/GluL3Val) of the Fab-fragment showed an increased affinity towards the hapten H/Cl/C6 (4-amino-6-chloro-l,3,5-triazine-2-(6-aminohexanecarboxylic acid) compared to the affinity of the wild-type Fab-fragment towards the same hapten. However, the mutant exhibited substantially lower affinity towards the hapten H/Cl/C6 than towards atrazine and the hapten iPr/Cl/C6 (4-chloro-6-(isopropylamino)-1,3,5-triazine-2-(6-aminohexanecarboxylic acid), which is usually used in the synthesis of enzyme tracers in ELISA for atrazine. Advantage was taken of the low cross-reactivity and increased affinity of the mutant Fab fragment towards H/Cl/C6 to improve the sensitivity of a direct-competitive ELISA tor atrazine. H/Cl/C6 was covalently conjugated with horseradish peroxidase (HRP), and the conjugate H/CI/C6-HRP was used as enzyme tracer in the ELISA for atrazine. An eight-fold improvement in sensitivity of a direct-competitive ELISA for atrazine could be achieved using the tracer H/Cl/C6-HRP compared to the sensitivity of ELISA using the tracer iPr/Cl/C6-HRP. The detection limit for atrazine was as low as 0.01 i g/l.
An Application of Reverse Transcriptase Polymerase Chain Reaction in A Relative Quantification of Gene Expression Santoso, Adi
Annales Bogorienses Vol. 9 No. 1 (2004): Annales Bogorienses
Publisher : BRIN

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Abstract

The ability to quantify steady state levels of individual messenger-RNA (mRNA) transcripts has been the key issue for study on the control of gene expression. Although two available techniques, Northern blot and nuclease protection assays (NPA) have been widely used tor detecting mRNA, these techniques have critical limitations. The most obvious limitation of these two techniques is the required number of target mRNAs to be detected. Reverse transcription-polymerase chain reaction (RT-PCR), which has been accepted as a highly sensitive and specific method, provides a means for detecting and quantifying gene expression using, theoretically only a single molecule of mRNA. The sensitivity and reliability of RT-PCR is dependent upon both the RT and PCR steps. The PCR step has been problematic because of the exponential nature of this reaction where small variation can lead to dramatic changes in final result. Therefore, the use of RT-PCR for quantification of gene expression requires pre-experimental planning and design. In this experiment, the procedure for pre-experimental planning, linear range determination and subsequent relative quantification of gene expression are described in detail. A study of ornithine decarboxyJase gene, a gene involved in the polyamine biosynthesis and temporally expressed, during embryogenesis of Musca domestica (housefly) was used as the model. The results show that during early embryogenesis (t-1 to t-4) the expression level was very low. The increase in expression profile was observed started at t-5, peaked at t-9, and followed by substantial decrease from t-10 to t-12.
Maternal Contribution in Revealing The Effects of Methoxyacetic Acid (MAA) Administered Before Implantation on The Embryonic Development of Swiss Webster Mice (Mus musculus) Kaiin, Ekayanti M; Sumarsono, Sony H.; Surjono, Tien W.; Sudarwati, Sri
Annales Bogorienses Vol. 9 No. 1 (2004): Annales Bogorienses
Publisher : BRIN

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Abstract

Maternal contribution to and direct action of methoxyacetic acid (MAA) on the embryonic development bad been examined by conducting embryo transfer. To reveal the maternal contribution, compacted morulae and early blastocysts, which were collected from untreated Swiss Webster donor mice on day 3 of gestation, were transferred to day 2 pseudopregnant recipients, after having been treated with 2.0 mmo/lkg body weight (b.w.) MAA by gavage on day 1 of pseudopregnancy. Direct effect of MAA on the embryonic development were observed by transferring compacted morulae and early blastocysts, similarly recovered from day 3 pregnant donor mice, after MAA treatment on day 2 of gestation with the same method and dosing, to untreated day 2 pseudopregnant recipients. Control donor mice and recipient were given distilled water only as the MAA solvent. Observations on fetuses resulting from embryo transfer wert: carried out on day 16 of gestation . Administration of MAA to the donors tended to decrease the unplantatlon rate and the survival rate of the implanted embryos. When MAA was given to the recipients the implantation rate and survival rate of embryos transferred decreased significantly (p<0.05) but the survival rate of implanted embryos were significantly higher (p<0.05) Lf compared to those of MAA treated donors. The intrauterine death tended to inc rease either in the treated donors or recipients. There was no effect of MAA on the fetal body weight and in producing fetal malformations. It is concluded that at the beginning of implantation, maternal contribution in revealing the effects of MAA on the embryonic development of Swiss Webster mice is predominant, whereas after implantation took place, the quality of the embryos become more important for their survival.
The Use of 16S rRNA and nodC Gene Sequence in Resolving The Phylogenetic Relationship of Rhizobia Associated With Paraserianthes falcataria (L.) Nielsen Plant Prana, Titik K; Sone, Teruo; Kawasaki-Nakagawa, Hiroko; Yokota, Atsushi; Seki, Tatsuji; Tomita, Fusao
Annales Bogorienses Vol. 9 No. 1 (2004): Annales Bogorienses
Publisher : BRIN

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Abstract

Studies on genetic position of several isolates living symbiotically on Paraserianthes falcataria have been carried out using amplification and sequencing techniques of 16S rDNA and specific gene for nodulation. The phylogenetic tree constructed based on 16S rDNA showed that rhizobia growing symbiotically on Paraserianthes falcataria consisted of 3 groups. The first group, was fast growing rhIzobia which have close relationship to Rhizobium tropicii, the second and the third groups were slow-growing rhizobia which have close relationship to Bradyrhizobium elkanii and Bradyrhizobium japonicum. However, the phylogenetic tree constructed on the basis of partial nodC gene indicated the existence of an independent group, since they did not show any significant degree of relationship with the existing groups. This was also supported by differences in physiological characteristic i.e Indole Acetic Acid production and salt tolerance of the isolates. These differences shows us that direct sequencing method of certain specific genes could give a more specific result than would display more clearly the degree of relationship at species level.

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