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Annales Bogorienses
Published by Badan Riset dan Inovasi Nasional
ISSN : 05178452     EISSN : 24077518     DOI : https://doi.org/10.55981/ann.bogor
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Annales Bogorienses aims to disseminate high-quality scientific research in the field of life sciences, with a strong emphasis on advancing knowledge and applications in biotechnology, molecular biology, biochemistry, bioinformatics, and bioengineering. The journal serves as a platform for researchers, academicians, and practitioners to share original findings, innovative methodologies, and critical reviews that contribute to scientific progress and sustainable development. The journal covers research in biotechnology, molecular biology, biochemistry, bioinformatics, and bioengineering. It publishes original research articles, reviews, and short communications, and is committed to rigorous peer review and open access for the widest possible dissemination of scientific knowledge.
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Articles 6 Documents
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Search results for , issue "Vol. 9 No. 2 (2004): Annales Bogorienses" : 6 Documents clear
EDITOR'S PREFACE Lisdiyanti, Puspita
Annales Bogorienses Vol. 9 No. 2 (2004): Annales Bogorienses
Publisher : BRIN

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Abstract

The editors would like to announce that, in the year of 2002 and 2003, The Research Centre for Biotechnology, The Indonesian Institute of Sciences (LIPI) did not publish Annales Bogorien es New Series due to some management problems. The last issue was published with Volume 8, Number 2, 2001. Under new editors, in this year of 2004, continuing the previous volume thi journal is published again starting with Volume 9, Number 1. In this ninth edition of Annales Bogorienses, we would also like to introduce a new I ok of this journal to our readers. We will continue to publish this journal periodically twice a year (in June and December).Many thank to all the author for their efforts, attention and time throughout the editing process that make this current edition become possible. Tolerance of our editorial judgment is mu h appreciated. We encourage our readers to submit original papers on topics of interest to life sciences researcher . December, 2004M. Ahkam Subroto Chief Editor
Modification of Plasmids and Cry Genes of Bacillus thuringiensis subsp. kurstaki HD-I After Treatment With Ethylmethanosulfonate (EMS) and UV Light Jusuf, Eddy
Annales Bogorienses Vol. 9 No. 2 (2004): Annales Bogorienses
Publisher : BRIN

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Abstract

Bacillus thuringiensis subsp. kurstaki HD-1 is a potential insecticidal bacterium producing five type of a-endotoxin crystal proteins. This bacterium is widely commercialized due to its wide spectrum toxicity against both Lepidopteran and Dipteran larvae. The objective of this work was to create autolysin deficient mutant causing cell fails to lyse. This mutant yield intact cells within spore and a-endotoxin crystal protein protected inside, from which an undamaged active bio-insecticide would be obtained. Two methods of mutagenesis, 2% of ethylmethanosulfonate and 10, 25, and 50 second of UV light exposure, resulted in mot (loss of motility) mutation. Observation showed that 14 of 20 survived mutants have lost some of its plasmids (varied from one to five). while the other six maintained their plasmids. By employing the polymerase chain reaction (PCR) the change on the cry genes was studied.
Cytological Analysis of Root Cultures of Artemisia cina Ermayanti, Tri Muji; Yanti, Oktavia; Hafiizh, Erwin Al
Annales Bogorienses Vol. 9 No. 2 (2004): Annales Bogorienses
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Artemisia cina is a medicinal plant species producing bioactive compounds which are potential a antitumor, antifungal and antibacterial. The aim of this study was to analyze the stability of chromosome number in root cultures of A. cina. Transformed root culture was established by infection of leaves of A. cina with Agrobacterium rhizogenes strains 07-20001, ATCC 15834, A4; and Agrobacterium tumefaciens strain R1000. Roots isolated from glasshouse plants, plantlets grown in solid and liquid MS medium were utilized for investigation of chromosome examination of untransformed roots. Chromosome examination was conducted by squashing method and chromosome numbers were calculated under microscope. The results showed that both untransformed and transformed root had in lability in the chromosome number, but had the modal number of chromosome x=8 with the diploid number of 2n =4x = 32. Roots isolated from glasshouse plants of A. cina had 53.7~ of cell with the diploid numbers of 2n = 3 and 46.3% of cells had chromosome numbers ranged from 2n = 12 to 2n = 64. Untransformed roots isolated from plantlets cultured in solid medw had only 36.1% or cells with chromo orne number of 20 = 32, and untransformed roots grown in liquid medium had 49.4% of cells with 2n =32. The chromosome numbers of A. cina transformed roots was affected by trains of Agrobacterium. Root transformed with the bacterium Strain 07-20001 showed the highest in normal chromosome number of 2n = 32 (62.4%) followed by roots transformed with strains ATCC 15834 (61.9%). R1000 (43.6%) and A4 (43.0%). The range of the chromosome number of untransformed roots was from 2n=17 to  2n=64 whilst that of transformed roots was from 2n=11 to 2n=66.
Denitrification of Activated Sludge in The Presence of Different Organic Substrates Agustiyani, Dwi; Yamagishi, Takao
Annales Bogorienses Vol. 9 No. 2 (2004): Annales Bogorienses
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The effect of organic carbon denitrifying activity was studied in batch reactor. Fom reactors were operated in parallel under anoxic condition in four different donor electrons. which were acetic acid (Reactor A), methanol (Reactor M), phenol (Reactor P), and glucose (Reactor G). The reactors were fed with the artificial waste, which contain 721.8 mg/l NaNO3. The concentration organic carbon added to the reactors were varied from TOD:N ratio of 0.5:1; 1: 1, 1.5:1, to 2:1. The denitrification activity was estimated by measuring the reduction rate of nitrogenous oxide and N2O gas production. The denitrification capacity of adapted-sludge was also investigated, and the rates were estimated from the cumulative N2O (without acetylene inhibition) and N2 gas production. Reduction rate of nitrogenous oxide in all reactors increased during the investigation; lhe increase reduction rate were correlated to the increase of organic carbon concentration. The maximum reduction rate of nitrogenous oxide in Reactor A was higher than those of the others. However, reduction rate in Reactor M was more constant, so that nitrogenous oxides existed in this reactor was removed faster. The highest potential denitrification rate (N20 production) was observed in sludge of Reactor A. However, N2 gas recovery trom nitrate and nitrite transformed by sludge of Reactor M was the highest. Linear correlation between nitrogenous oxide reduction with gas production was observed in Reactor A, M and P, but not in Reactor G.
Development of Somatic Embryo in Lithospermum erythrorhizon Siebb. et Zucc and The Study on The Effect of Methyl Jasmonate on its Maturation Mariani, Totik Sri; Ramayanti, Octavia; Yazaki, Kazufumi; Miyake, Hiroshi
Annales Bogorienses Vol. 9 No. 2 (2004): Annales Bogorienses
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Abstract

A research on the somatic embryogenesis in Lithospermum erythrorhizon has been conducted. Embryogenic callus was inoculated in EIM9 liquid medium (modification of LS medium), i.e. L2PVP (initiation medium) and the development of somatic embryo was observed. Ten uM and 100 uM methyl jasmonate was added into L3PVP medium (differentiation medium) for somatic embryo maturation. The purposes of this research were to observe the development of somatic embryo and to observe the effect of methyl jasmonate on maturation of the somatic embryo in L. erythrorhizon. The results showed that somatic embryogenesis in L. erythrorhizon derived from single cells differentiated further forming proembryo, globular heart, torpedo and cotyledon stage. Treatment with 10 uM and 100 uM methyl jasmonate induced maturation of somatic embryos (cotyledon stage) after transferring them to embryo development medium (L4PVP).
PCR Amplification of Ornithine Decarboxylase (ODC) Gene Fragment from Tobacco (Nicotiana tabacum L.) cv. Temanggung Djajanegara, Ira; Pambudi, Sabar; Lestari, Retno; Artanti, Nina
Annales Bogorienses Vol. 9 No. 2 (2004): Annales Bogorienses
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Abstract

In order to create an antisense construct of the gene encoding Ornithine Decarboxylase (ODC) from tobacco (Nicoticum tabacum L.) cv. Temanggung, the target gene must be isolated. In this paper. we present the PCR amplification of a fragment from putative gene encoding ODC from tobacco cv. Temanggung. Leaf genomic DNA was isolated and used as the template for PCR. PCR optimization was done by adjusting the annealing temperature and the cycle number. Verification of the fragment obtained was also done using the second primer pairs.

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