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BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Published by Southeast Asian Regional Centre for Tropical Biology
ISSN : 02156334     EISSN : 1907770X     DOI : http://dx.doi.org/10.11598/btb
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary
BIOTROPIA, The Southeast Asian Journal of Tropical Biology, is a scientific publication of the Southeast Asian Ministers of Education Organization (SEAMEO) – Southeast Asian Regional Center for Tropical Biology (BIOTROP). It publishes articles in the broad areas of tropical ecosystems and environmental impacts, biodiversity conservation and sustainable development and biotechnology.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 6 Documents
 1 
Search results for , issue "Vol. 19 No. 2 (2012)" : 6 Documents clear
POSTHARVEST QUALITY IMPROVEMENT OF SORGHUM (Sorghum bicolor (L.) Moench) GRAINS OKKY SETYAWATI DHARMAPUTRA
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 19 No. 2 (2012)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.92 KB) | DOI: 10.11598/btb.2012.19.2.255

Abstract

The objectives of this study were (a) to investigate the effect of postharvest handling (threshing and storage) methods on the quality of sorghum (Sorghum bicolor (L) Moench) grains variety Numbu, in terms of the percentages of damaged grains and seed germination, population growth of S. zeamais, F. proliferatum and F. verticillioides; fumonisin B1 and carbohydrate contents, and the percentage of weight loss during storage. The change of moisture contents of sorghum grains was also recorded. Threshing was conducted using a stick of wood and a paddy thresher. Sorghum grains were packed in hermetic plastic bags. The conditions inside of the bags were  airtight and normal conditions. Each bag with different conditions inside of the bag was introduced with 10 pairs of Sitophilus zeamais (1-14 days old). Sorghum was stored for one, two and three months of storage under warehouse conditions. The results showed, that during storage the moisture contents of sorghum were  lower than that its standard safe moisture content (
ENDIANDRA KASSAMENSIS (LAURACEAE), A NEW SPECIES FROM NEW GUINEA Deby Arifiani
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 19 No. 2 (2012)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (94.458 KB) | DOI: 10.11598/btb.2012.19.2.289

Abstract

A new species of Endiandra (Lauraceae) is described from New Guinea. Endiandra kassamensis is described based on specimens collected over four decades ago. Unlike most Endiandra which grow in lowland forest, E. kassamensis is found in high altitude forest. The species is characterized by the presence of staminodia with the absence of staminal glands.   Key words: Endiandra, Lauraceae, staminal glands, staminodia, endemic, New Guinea
Mitochondrial DNA variation of the Sumatran elephant in Sumatera Sri Sulandari
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 19 No. 2 (2012)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.481 KB) | DOI: 10.11598/btb.2012.19.2.250

Abstract

A research on Mitochondrial DNA analysis of genetic diversity in Sumatran elephant (Elephas maximus sumatranus) was conducted in this study.  A 630 bp segment of mitochondrial DNA was amplified on 105 samples of Sumatran elephant from 5 locations in Sumatera (Bentayan, Sugihan, Bukit Salero Lahat, Seblat, Way Kambas) using a set of primers: MDL3 (5’-CCCACAAT-TAATGGGCCC-GGAGCG-3’) and MDL5 (5’-TTACATGAATTGGCAGCCA-ACCAG-3’). The objectives of this study is to generate mitochondrial DNA D-loop sequences for all the Sumatran elephant samples under this study and  to provide information haplotypes and nucleotide diversity of  Sumatran elephant populations.             A total of 105 PCR product were successfully sequenced perfectly, with an average length of about 616 base pairs. However, mitochondrial DNA fragments  for this analysis used the first 601 bases. Results showed six haplotypes (BP, BT, BS, BR, BX and BY) identified in Sumatera.  The most of the sampled individuals are the haplotipe BT. BX and BY are most likely new haplotypes..  All haplotype, except for the haplotipe BP  are belonging to the Sumatera clade. The haplotipe BX was derived from the haplotipe BT, and the haplotype  BY was derived from the haplotipe BS by one transversion respectively. The other substitutions in this network were the transitions. The haplotype BP is widely distribute from Sri Lanka, Sumatera, Peninsular Malay  and China). Although reported that the haplotype BU distributed Sumatera and Peninsular Malay, but BU haplotype not detected in this study.             Genetic distances within populations in Bentayan, Bukit Salero Lahat, Seblat, Sugihan and Way Kambas ranged from 0.0000 - 0003, and the genetic distance between the populations that is 0.0000 - 0. 0022. The distance between haplotypes of Sumatran elephant’s population is low.• The diversity of haplotypes and nucleotide in Sumatera island is low, the highest is  in the region of Buki Salero  Lahat and, lowest is in Bentayan and Sugihan. Overall, the results of analysis of Fu and Li's F * test statistic indicates that the population of Sumatran elephants in Sumatra is -0.78871, which means there is no inbreeding, but not significant at P> 0:10.   Keywords : Sumatran elephant, Elephas maximus  sumatranus, mitochondrial DNA, haplotype
THE NATURAL HABITAT OF BEGONIA ON THE ISLAND OF BALI: MICROCLIMATE PREFERENCE IN TWO FOREST SITES IN BEDUGUL Annisa Satyanti
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 19 No. 2 (2012)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2012.19.2.237

Abstract

A study on Begonia’s ecology was conducted in two forest sites, a nature reserve and reboisation forest, respectively, in Bedugul Bali. The objective of the study was to describe Begonia species found in respective forest sites, to gather information on abiotic factors and to find the influences of these abiotic factors to the abundance of Begonia species. Three species were identified as B. multangula, B. baliensis and B. longifolia. There were two form of B. longifolia, white and red. The multivariate analysis (PCA) showed that abiotic factors measured were relatively similar among plots. In addition, no particular abiotic factor, from multivariate analysis, showed specific influence on Begonia species frequency.  Interestingly, the multivariate analysis also implied that B. latifolia has different microclimate preference between forms (white and red).  The abundance, refers to the frequency, of all of species in two forests was similar (t-test, p=0.061). Pooling the data from the fourty plots (two forest sites) and white and red forms of B. longifolia together, B. multangula and B. longifolia found were similarly abundant between species (t- test, p=0.460).
VEGETATION OF LOWLAND TROPICAL FOREST (WEST PAPUA), HUMAN PRESSURE, FOOD AVAILABILITY AND WALLABY (Dorcopsis muelleri) PRESENCE Karlè Sykora
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 19 No. 2 (2012)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2012.19.2.239

Abstract

The presence of Dorcopsis muelleri in different habitats and related vegetation types has been studied in lowland tropical vegetation in the northern Part of Manokwari, West Papua. Four habitat types comprising 6 plant communities could be distinguished:  1. Grassland, Imperata cylindrica - Ipomea aquatica community; 2. Open forest: a. open forest transitional to grassland, Ficus robusta - Dendrochide sp. community, b. open forest after logging, Musa paradisiaca – Callamus longipina community, c. open forest regenerated after logging,  Diospyros hebecarpa-Lepinopsis ternatensis  community, d. open forest on rocky soil, regenerated after logging, Smilax malacensis-Pandanus tectorius community; 3. Undisturbed (“primary) closed forest, Sommeria leuchophylla–Paraltropis glabra community.  The forest wallaby has especially been noted in grassland, in open forest with only little logging activity and in undisturbed closed forest. It was however not registered from the forests with clear logging influence. D. muelleri appears to be very sensitive to human disturbance. Our results indicate that vegetation structure, food plant availability and possibly also vegetation composition is less important than human disturbance. There is a strong negative relation between on one hand presence of the forest wallaby and on the other hand logging, distance to villages and hunting.
Callus induction of cottoni seaweed (Kappaphycus alvarezii (Doty) Doty) collected from Natuna Islands, Riau Islands Province. Erina Sulistiani
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 19 No. 2 (2012)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2012.19.2.254

Abstract

The objective of this study was to obtain the optimal medium for callus induction from thallus explants off Kappaphycus alvarezii (Doty) and to regenerate filamentous callus from induced callus. Before cultured cottonii seaweeds collected from the Natuna Islands (Riau Islands Province) were acclimatized in greenhouse and in semi-sterile culture in the laboratory. Sterilized explants were cultured on PES and Conwy media solidified with 0.8% Bacto Agar. In each of these media two combinations of plant growth regulators i.e. BA+IAA and BA+NAA were added. The concentrations of BA used were 0, 0.5, 1 mg/l, the concentrations of IAA were 0, 2.5, 5 mg/l, whereas the concentration of NAA were 0, 0.5, 1 mg/l. The result indicated that the optimal medium for callus induction was PES solidified medium supplemented with BA 1 mg/l. Types of callus formed were (a) white compact callus, (b) white filamentous callus, (c) greenish/brownish callus. Regeneration of callus into clumps offilament had been done by subculturing the callus into PES solidified medium supplemented with BA 1 mg/l + IAA 2.5 mg/l

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