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The Journal of Experimental Life Sciences (JELS)
Published by Universitas Brawijaya
ISSN : 20872852     EISSN : 23381655     DOI : 10.21776/ub.jels
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience
The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 11 Documents
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Search results for , issue "Vol. 9 No. 3 (2019)" : 11 Documents clear
Phytochemical Screening and Antibacterial Activity of Kratom Leaf (Mitragyna speciosa Korth.) Against Aeromonas hydrophilla Juanda, Eki; Andayani, Sri; Maftuch, Maftuch
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1030.866 KB) | DOI: 10.21776/ub.jels.2019.009.03.02

Abstract

Kratom (Mitragyna speciosa) is an indigenous tropical herbal plant to the Northern Malay Peninsula and Thailand. Empirically kratom leaves have several properties as herbal medicines. Currently, the treatment of diseases caused by bacteria that are resistant to antibiotics requires new compounds that have high potential. The material studied was kratom leaf extract. The solvent used for extraction is methanol. Phytochemical screening carried out includes the examination of alkaloids, flavonoids, steroids/terpenoids, phenols, tannins, and saponins. The test bacteria used was Aeromonas hydrophilla. The medium used is Nutrient Agar (NA). NaCl 0.9% as a bacterial suspension. Testing the antibacterial activity carried out with 10 (ten) variations of concentration namely 3%, 6%, 9%, 12%, 15%, 18%, 21%, 24%, 27% and 30 %. Fresh kratom leaf is collected and then wet sorted. The extraction process was using the method of maceration. This study aims to analyze the antibacterial activity of kratom leaf, measure the secondary compounds of kratom leaf extract, and measure the best concentration to kill Aeromonas hydrophilla. The extraction process obtained blackish-brown extract with 62.27 grams of extract (31.14%). Secondary metabolites of kratom leaf extract show positive results of alkaloid, saponins, tannins, phenolics, steroids, and triterpenoids. Antibacterial activity against Aeromonas hydrophila which is characterized by a kill zone around the paper disc with the best concentration that shows a wide killing zone was 24% of the extract.Keywords: Antibacterial, Extract, Kratom, Mitragynine, Phytochemical Screening.

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