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PUSAT PENELITIAN DAN PENGABDIAN PADA MASYARAKAT POLITEKNIK PERTANIAN NEGERI KUPANG JL. PROF. DR. HERMAN YOHANES KEL. LASIANA, P.O. BOX 1152, KUPANG 85011 TELP. (0380) 881600, FAKS. (0380) 881601
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INDONESIA
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Published by Politeknik Pertanian Negeri Kupang
ISSN : 08526877     EISSN : 25273981     DOI : 10.35726/jp
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Social Sciences Veterinary
Jurnal Pertanian Terapan PARTNER menerima artikel hasil-hasil penelitian dalam bidang pertanian terapan maupun critical reviews yang berhubungan dengan: Produksi Ternak Nutrisi dan Pakan Ternak Teknologi Pakan Ternak Kesehatan Hewan Sosial Ekonomi Pertanian/ Peternakan Agronomi Perlindungan Tanaman Ilmu Tanah Kehutanan Teknologi Hasil Pertanian Desain atau Rancang Bangun Model Pertanian berbasis Pertanian Lahan Kering Namun demikian, jurnal ini lebih difokuskan pada penelitian-penelitian dalam rangka pengembangan pertanian lahan kering.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 10 Documents
 1 
Search results for , issue "Vol 24, No 1 (2019): Edisi Juli" : 10 Documents clear
OPTIMAL PROTEIN Cj0391c EXPRESSION AND PURIFICATION METHODS FOR PROTEIN IDENTIFICATION Erda Eni Rame Hau
Partner Vol 24, No 1 (2019): Edisi Juli
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v24i1.331

Abstract

Identifikasi mekanisme virulensi yang terkait dengan Campylobacter jejuni adalah bidang yang penting karena merupakan target potensial untuk vaksin masa depan. Contoh protein yang disekresikan oleh C. jejuni yang telah dipelajari baru-baru ini adalah Cj039lc.  Analisa bioinformatika menemukan bahwa protein ini bisa menjadi ?-helical pore forming protein (PFP).  Untuk mengetahui lebih lanjut mekanisme virulensi dari protein ini maka perlu dilakukan identifikasi lebih mendalam tentang protein.  Oleh karena itu penelitian ini bertujuan untuk mengidentifikasi metode ekspresi dan purifikasi yang optimal untuk menghasilkan protein Cj3091c yang murni dan cukup, yaitu 1 mg/ml Cj0391c murni.  Pemurnian dilakukan dengan Iminobilized metal Affinity Chromatography (IMAC) kolom yang digunakan untuk memulihkan protein rekombinan. SDS-PAGE dan Western blotting digunakan sebagai metode untuk menentukan kondisi optimum untuk elusi protein rekombinan Cj039lc.  Hasil dari ekspresi dan pemurnian pada percobaan ini menyimpulkan bahwa konsentrasi optimum Imidazole dalam buffer pencuci untuk menghasilkan konsentrasi tinggi protein Cj039lc murni adalah kombinasi dari langkah awal 20 mM buffer pencuci Imidazole diikuti dengan dua kali mencuci menggunakan 40 mM Imidazole. Konsentrasi akhir l mg/mL Cj039l c kemudian siap untuk digunakan untuk Small-angle X-ray scattering (SAXS) dan Dynamic light scattering (DLS).
CRISPR-CAS9 SYSTEM: A NEW DEFINING ERA IN AGRICULTURE? Stormy Vertygo
Partner Vol 24, No 1 (2019): Edisi Juli
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v24i1.330

Abstract

Revolusi dalam bidang rekayasa genomik terjadi sejak dikembangkannya sistem CRISPR-Cas9 yang diperoleh dari mekanisme pertahanan bakteri. Proses ini melibatkan pengikatan fragmen RNA penuntun (gRNA) yang spesifik pada DNA target yang akan menginisiasi aktivitas katalik enzim endonuklease (Cas9) pada bagian DNA tersebut. Induksi perubahan ini akan menggerakkan sistem reparasi seluler yang menghasilkan struktur genetik baru dengan karakteristik fenotipik yang diinginkan. Karena kepraktisan, fleksibiltas dan keakuratannya, teknologi ini semakin terintegrasi dalam berbagai bidang ilmu penelitian, terutama Agrikultur. Meskipun penerapan aplikasinya masih bersifat eksperimental di dalam laboratorium, hasilnya telah menunjukkan prospek yang menjanjikan di masa mendatang. Namun, banyak aspek yang masih perlu dipertimbangkan sebelum berbagai produk hasil teknologi ini di-komersilkan di pasaran. Dalam review ini, ulasan mengenai CRIPSR-Cas9 akan menjadi fokus perhatian disertai contoh penerapan mutakhirnya dalam budidaya tanaman pangan, produksi ternak dan kesehatan hewan. Kemungkinan efek negatif baik bagi manusia dan juga lingkungan dalam jangka panjang juga akan menjadi bagian dari pembahasan.Kata kunci: CRISPR-Cas9, Rekayasa Genomik, Agrikultur.
Analysis Jaringan Sosial Perdagangan Ternak Babi Terhadap Penyebaran Penyakit Classical Swine Fever atau Hog cholera di Kabupaten Sikka Provinsi Nusa Tenggara Timur (NTT), Indonesia. Petrus Malo Bulu; Ewaldus Wera; Margaretha Sikko
Partner Vol 24, No 1 (2019): Edisi Juli
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v24i1.361

Abstract

Classical Swine Fever (CSF) is a fatal viral disease of both domestic pigs and wild boar. This disease has become endemic in some districts in Nusa Tenggara Timur. The disease has caused huge economic lossess to pig farmers in NTT including in the district of Sikka in 2017 that was estimated at approximately sixty billion rupiah. Little is known how this disease introduced and transmitted into the region. This research was aimed to identify and analyze the movement of pigs through the market chain as a pathway to spread the disease from outside and within the district of Sikka.A total of 57 respondents were interviewed in this study involved sellers and buyers in the markets (4 buyers and 4 sellers in each market-a total of 32 people), suppliers of pigs (10 people), and pig farmers as many as 5 respondents per village (3 villages selected purposively). A face-to-face interview was conducted to obtain information from the respondents.This study found 5 actors that had the highest Out-Degree values including S5, D1, F24, K1, and P1. The study also found four actors that had the highest betweeness centrality in the network including actors D1, D16, F3 and F30 with their degree of Betweenness centrality of 1532,4, 1265.1, 823.5, dan 738.1 respectively. The network density was 0,12, and diameter was 7. Average geodesic distance was 2,94 (1.26), and overall clustering coefficient of the network was 0,61. The in and out-degree values in the current study suggest that pigs were moving in and out of the areas through the movement of sellers and buyers. The results of the study found that market sellers and buyers are actively moving through the market network, and therefore could potentially contribute to the spread of CSF in Sikka, if an outbreak occurred in the region. This study also found that there was potential movement of pigs in different villages in Sikka through the directed links or ties, which could contribute to the transmission and spread of CSF.
PENGARUH PEMBERIAN PAKAN ALTERNATIF LIMBAH IKAN ASIN DAN TEPUNG KEDELAI TERHADAP PERTUMBUHAN IKAN LELE DUMBO (Clarias gariepinus) andri jaya kesuma
Partner Vol 24, No 1 (2019): Edisi Juli
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v24i1.338

Abstract

The production sector of Clarias gariepinus every year has increased. One obstacle to the cultivation of Clarias gariepinus is the high cost of commercial feed. This study aims to determine the effect of alternative feeding of salted fish waste and soy flour to the growth of Clarias gariepinus. This research was conducted in December 2018 - January 2019. The parameters observed in this study were the length and weight of Clarias gariepinus. The study was conducted using the Rancangan Acak Lengkap (RAL) method using 6 treatments, one of which was as a control and was repeated three times. The results of this study indicate the effect of alternative feeding on the growth of Clarias gariepinus. The best feed was in P2 treatment with a concentration of 75% salted fish meal flour and 25% soy flour, with an absolute length of 5.33 cm, weight of 7.66 grams and the lowest conversion value was 141.
KUALITAS OOSIT KAMBING KACANG DARI STATUS REPRODUKSI YANG BERBEDA Hermilinda Parera
Partner Vol 24, No 1 (2019): Edisi Juli
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v24i1.384

Abstract

This research was aimed to determine the quality of oocytes produced by Kacang goat based on reproductions cycle per ovary. Ovaries were obtained from the slaughterhouses in Kupang. The oocytes were collected by aspiration method, then selected based on quality. The result showed that 300oocytes from 47 ovaries, then grouped into quality of A, B, C, and D, have the average weight with reproduction status of1,56g (+CL+FD), 1,50g (+CL-FD), 1,45g (-CL+FD), 1,45g(-CL-FD), respectively. The percentage of oocyte with quality A, B, C, and D between reproduction status (+CL+FD)(+CL-FD)(-CL+FD)(-CL-FD) (7,3%, 21,05%, 13,6%, 32,63%); (8,2%, 10,3% 20,6%, 53,6%); (7,69%, 7,69%, 41,02%, 43,58%); (7,2%, 10,14%, 33,3%, 49,2%, recpectively). There is no significant difference (P>0,05) between the quality of oocytes from different ovarian status.It can concluded that the average weight of Kacang goat ovaries were 1,45-1,56g.The ovarian reproductive status does not affect the quality of the oocytes. Key words: Reproduction cycle, oocytes quality, ovaryKacang goats, Kupang city.
JENIS MINERAL DAN SIFAT KIMIA TANAH VERTISOL HASIL PELAPUKAN BATUAN GAMPING DI KABUPATEN JENEPONTO masria ria masria
Partner Vol 24, No 1 (2019): Edisi Juli
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v24i1.350

Abstract

This study aims to study the types of minerals and chemical properties of Vertisol soil that develop from limestone rocks in Jeneponto Regency, South Sulawesi. Taking soil samples is carried out at four different depths, namely: 0-15 cm, 15-30 cm, 30-45 cm,  and 45-60 cm. Observation parameters for soil mineral types are primary minerals and secondary minerals, while soil chemical properties observed are pH, C-organic, cation exchange capacity (CEC). The results showed that the dominant primary mineral species were calcite and quartz, the dominant secondary mineral species was montmorillonite. Soil chemistry for soil pH ranges from 6.58-6.85, C-organic ranges from 2.23-2.81% or moderate status, and CEC ranges from 19.98 to 22.76 cmol (+) / kg or moderate status.
PENILAIAN ORGANOLEPTIK FERMENTASI PAKAN KOMPLIT TERNAK KAMBING BERBASIS PUTAK DAN TEPUNG IKAN bambang hadi sutanto
Partner Vol 24, No 1 (2019): Edisi Juli
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v24i1.349

Abstract

This study aims to utilize the availability of local food such as gamal leaves, corn klobot, putak, rice bran and fish meal through a touch of fermentation technology. The design used in this study was a completely randomized design with 4 treatments and 5 replications. This study assessed organoleptic fermentation of complete feed with 0, 3, 6 and 9 weeks fermentation time in anaerobic conditions with organoleptic research variables (color, aroma, texture) Organoleptic results showed that the fermentation period of 3 weeks and 6 weeks (33.3%) showed the brightest color of complete fermented feed, 9 weeks fermentation time showed the most preferred aroma level (55.5%), 6 weeks fermentation time showed texture the softest (55.5%). The conclusion of acceptance of complete feed fermentation time to color, aroma and texture was fermentation time for 6 weeks (44.4%).
KONSUMSI BAHAN KERING DAN BAHAN ORGANIK KAMBING KACANG JANTAN YANG DIBERI NAUNGAN DAN TANPA NAUNGAN Merianse Djita; Bambang H. Sutanto; Cardial L.O. Leo-Penu
Partner Vol 24, No 1 (2019): Edisi Juli
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v24i1.345

Abstract

This study used 14 Kacang male goats at same age. The animals were randomly divided into two groups, namely 1) the pen with shade and 2) and the pen without shade. The results showed that Kacang goats stayed in the pen with and without shade were not different in dry matter consumption (267.25 g/d/h and 266.84 g/d/h  repectively) and organic matter consumption(178.74 g/d/h  and 180.83 g/d/h repectively). In conclusion, farmed goat Kacang in the pens with and without shade have a relatively similar consumption of dry matter and organic matter. However, water consumption of the shaded goat group was much higher compared to the unshaded goat group.
CONSERVED REGION OF ELEPHANT ENDOTHELIOTROPIC HERPESVIRUS (EEHV) BASED ON BIOINFORMATICS ANALYSIS Ika Agus RINI
Partner Vol 24, No 1 (2019): Edisi Juli
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v24i1.336

Abstract

Gajah adalah salah satu hewan yang dilindungi di Indonesia. Endotheliotropic Herpesvirus (EEHV) merupakan salah satu penyakit yang menyebabkan kematian pada gajah. Hingga saat ini, belum banyak penelitian tentang penyakit tersebut, termasuk cara mendeteksi infeksinya. Penelitian ini bertujuan untuk mencari daerah lestari gen U38 dari DNA polimerase berdasarkan sekuens DNA di Gene Bank, sehingga dapat digunakan sebagai primer untuk deteksi dini penyakit yang disebabkan oleh infeksi EEHV pada gajah. Urutan genom EEHV dikumpulkan dari GeneBank, NCBI. Sekuens tersebut dianalisis dengan penyelarasan berganda menggunakan clustalX.2 dan dianalisis lebih lanjut dengan BioEdit. Beberapa daerah lestari yang ditemukan kemudian dianalisis lebih lanjut dengan Blastn untuk mengevaluasi daerah yang akan digunakan sebagai primer. Hasil penelitian menunjukkan bahwa satu sekuens memenuhi kriteria primer yang baik dan sesuai dengan standar yang umumnya digunakan. Sehingga, untuk kedepannya dapat digunakan sebagai kandidat dalam deteksi EEHV pada gajah.
PUPUK HAYATI (BIOFERTILIZER) ALTERNANTIF SUBTITUSI PENGGUNAAN PUPUK KIMIAWI asrul asrul asrul
Partner Vol 24, No 1 (2019): Edisi Juli
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v24i1.340

Abstract

Increased population growth implies the intensive management of agricultural land for food fulfillment. As a result, the use of chemical fertilizers among farmers continues to increase to support this intensive agriculture. UREA, NPK and Super Phosphate Fertilizers are chemical fertilizers that are used by farmers to meet the macro nutrients of plants, especially N, P and K. The problem is that excessive and intensive use of chemical fertilizers turns out to have a negative impact on both agricultural land and accumulated residues in plants which are then consumed by humans. One of the efforts to substitute chemical fertilizers in agricultural land by applying biofertilzer or biofertilzer. Biofertilizers are fertilizers that use the services of microorganisms (bacteria) to meet plant nutrient needs.

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