Article Per Year (5 Year)
p-Index From 2021 - 2026
1.204
P-Index
This Author published in this journals
All Journal African Multidisciplinary Journal of Sciences and Artificial Intelligence Open Access DRIVERset African Journal of Biochemistry and Molecular Biology Research Open Access DRIVERset African Journal of Sciences and Traditional Medicine Open Access DRIVERset Kwaghe International Journal of Sciences and Technology Open Access DRIVERset
Wurochekke, A. U.
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Nursing Public Health Social Sciences

Published : 8 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : Open Access DRIVERset

 1 
Purification and Characterization of Lactoferrin from Camel Urine Ahmad, K. A.; Jada, M. S.; Wurochekke, A. U.
African Journal of Biochemistry and Molecular Biology Research Vol 2 No 3 (2025): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v2i3.7705

Abstract

This study reports the isolation, purification, characterization, and antimicrobial activity of lactoferrin derived from camel urine. The lactoferrin was purified through a sequential process involving acetone precipitation, dialysis, ion exchange chromatography, and gel filtration. The partially purified protein was then characterized with respect to its molecular weight, pH and temperature optima, metal ion interaction, and thermal stability. The purification process achieved a 3.23-fold increase in purity with a specific activity of 6.33 U/mg protein and an overall yield of 9.74%. The purified lactoferrin exhibited a molecular weight of 75 kDa and demonstrated optimal activity at pH 7.0 and 40 °C in 50 mM sodium phosphate buffer. Among the metal ions tested, Zn²⁺ enhanced lactoferrin activity, while Mg²⁺ and Al³⁺ acted as strong inhibitors. These findings confirm the potential of camel urine as a novel and viable source of bioactive lactoferrin and lay the groundwork for further biotechnological exploration of its therapeutic applications.
Co-Authors Ahmad, K. A. Jada, M. S.