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All Journal Agrosains: Jurnal Penelitian Agronomi
Talitha, Okky
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Agriculture, Biological Sciences & Forestry

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Effect of Kinetin and 2,4-D Plant Growth Regulators on In Vitro Subculture Growth of Dendrobium stockelbuschii Talitha, Okky; Yamauchi, Kosei; Setyawati, Andriyana; Iqbal, Muhammad; Saskya, Nanda
Agrosains : Jurnal Penelitian Agronomi Vol 27, No 2 (2025): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agsjpa.v27i2.110517

Abstract

Dendrobium orchids are a popular genus due to their adaptability and wide variation in flower shapes and colors. Conventional propagation methods face challenges in producing large quantities of high-quality seedlings. This study aimed to produce superior-quality seedlings through tissue culture techniques using ½ Murashige and Skoog (MS) medium supplemented with kinetin and 2,4-D to enhance growth. The research was conducted at the Plant Physiology and Biotechnology Laboratory, Sebelas Maret University, from February to July 2024. The experimental design used was a completely randomized design (CRD) with two factors. The first factor was kinetin concentration (0, 0.5, 1, 1.5, 2 ppm), and the second factor was 2,4-D concentration (0, 0.5, 1, 1.5, 2 ppm). A total of 25 treatment combinations were tested, each replicated three times, resulting in 75 experimental units. Observed parameters included the number of roots, plant height, number of shoots, and root length. Data were analyzed using analysis of variance (ANOVA) at a 5% significance level, followed by Duncan’s Multiple Range Test (DMRT) for mean separation. The results showed that kinetin had a significant effect on the number of roots and plant height, while 2,4-D significantly affected root length. No significant interaction was observed between the two factors.
Effect of Water Availability on the Growth and Secondary Metabolites of Zodia (Evodia suaveolens) Setyawati, Andriyana; Purwanto, Edi; Yunus, Ahmad; Samanhudi, Samanhudi; Sakya, Amalia Tetrani; Rahayu, Muji; Purnomo, Djoko; Arniputri, Retna Bandriyati; Cahyo, Gani; Dwiyanti, Qonita Rahma; Egra, Saat; Talitha, Okky; Muthi’ah, Alfida
Agrosains : Jurnal Penelitian Agronomi Vol 25, No 2 (2023): Agrosains: Jurnal Penelitian Agronomi
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agsjpa.v25i2.81528

Abstract

Zodia are included in medicinal plants because it contains secondary metabolites such as flavonoids, tannins, alkaloids, steroids/triterpenoids that have many benefit and also as mosquito repellents. The growth and content of secondary metabolites in plants can be affected by environmental factors such as water availability. Research on the cultivation of this crop is still limited. Environmental modification in cultivation is needed to increase its secondary metabolites, and the study of the potential of this plant as medicine are necessary. This study aimed to determine the effect of water availability on the growth and secondary metabolites of zodia. The research was carried out in July-November 2021 in Greenhouse, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta. The method used was a completely randomized design, one treatment factor with 4 levels namely, 100, 80, 60 and 40% of field capacity. Each treatment was repeated 6 times so that there were 24 experimental polybags. The data analysis used is Analysis of Variance, and the Duncan’s Multiple Range Test at the 5% level. The results showed that the availability of water at a level of 60% field capacity gave the best average growth parameters (plant height and number of leaves) among other treatments. The flavonoid content in each treatment showed the same average. The results of the GCMS (Gas Cromatography and Mass Spectroscopy) test of zodia leaves showed that there were dominant bioactive compounds, namely ethylbenzene, octadecamethyl cyclononasiloxane, pentadecanoid acid and heptadecanoid acid. It is suspected that the content of these compounds has the potential as antifungal, antibacterial, antioxidant and anticancer.
Co-Authors Amalia Tetrani Sakya, Amalia Tetrani Arniputri, Retna Bandriyati Cahyo, Gani Djoko Purnomo Dwiyanti, Qonita Rahma Edi Purwanto Egra, Saat Muhammad Iqbal Muji Rahayu Muthi’ah, Alfida Samanhudi Samanhudi Saskya, Nanda Setyawati, Andriyana Yamauchi, Kosei Yunus, Ahmad