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Prevalensi dan Analisis Faktor Risiko Multidrug Resistance Bakteri Escherichia coli pada Ayam Komersial di Kabupaten Blitar Wibisono, Freshinta Jellia; Sumiarto, Bambang; Untari, Tri; Effendi, Mustofa Helmi; Permatasari, Dian Ayu; Witaningrum, Adiana Mutamsari
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol 10 No 1 (2020): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Vete
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (439.328 KB) | DOI: 10.46549/jipvet.v10i1.74

Abstract

Multidrug resistance is a problem that is difficult to overcome in terms of treating infectious diseases. Multidrug resistance is the term used to describe when a bacteria is resistant to three or more different classes of antibiotics. Escherichia coli as a commensal bacterium which has multidrug resistance, this causes more issues because Escherichia coli can transfer its resistant properties to other bacteria within the poultry digestive tract. The observational study is used to determine the risk factors and to estimate the quantitative effects arising from various components that contribute to the emergence of a disease. The sampling in this study was carried out randomly through cloaca swabs from commercial chicken farms in Blitar and 345 samples were collected. Complementary data collection was carried out using questionnaires, interviews, and field observations. The results showed the incidence of multidrug resistance in commercial chickens in the Blitar District was 72.5%. There is a relationship between causative factors with the incidence of multidrug resistance in Escherichia coli bacteria that is significantly associated with positive risk factors. The strength of this relationship can be seen from the value of OR and RR, among others factors of chicken breed (OR = 3.07; RR = 1.34), breeder's education (OR = 2.3; RR = 1.29), type of livestock business (OR = 7.5; RR = 1.43), type of animal feed (OR = 1.91; RR = 1.2), veterinary support for livestock raising management (OR = 3.09; RR = 1.44). The reference variables are whether the antibiotics are administered by non-veterinarians (OR = 2.35) or by the TS (OR = 7.92), and whether there is an antibiotic administration program (OR = 3.16; RR = 1.47). The overseeing function of farm maintenance, management, and implementation of antimicrobial administration in commercial chicken farms needs to be improved, to increase breeders' awareness of the careful usage of antibiotics and controlling the incidence of antibiotic resistance.
Hubungan teknik pemerahan dengan jumlah Escherichia coli pada susu segar dari peternakan sapi perah di KUD Kertajaya, Kabupaten Kediri, Jawa Timur Rahadyan, Raihan Alif; Tyasningsih, Wiwiek; Puspitasari, Yulianna; Permatasari, Dian Ayu; Widjiati, Widjiati
Current Biomedicine Vol. 1 No. 1 (2023): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.1.1.19-24

Abstract

Background: The coliform bacteria group is a group of bacteria used as an indicator of food sanitation, one of which is in dairy products. Objective: This study aimed to determine the relationship of milking techniques with the number of Escherichia coli in raw milk compared to the bacterial contamination limit set by the National Standardization Agency. Methods: The design of this study was a descriptive study. Raw milk samples were taken from Lucky Farm and several traditional dairy farms in the KUD Kertajaya area, Kediri Regency, East Java. This study uses the most probable number (MPN) test. Results: The results showed 11 (73.3%) of raw milk samples using conventional milking techniques exceeding the maximum limit of E. coli bacterial contamination determined by the National Standardization Agency (< 3 MPN/mL). However, all 15 samples, or 100% of raw milk samples using machine milking techniques, were negative for E. coli and met the maximum bacterial contamination limit requirements. Conclusion: The milking techniques affect E. coli contamination limits in raw milk. The milk samples taken using machine milking techniques had better results with MPN values ​​< 3 MPN/mL than those taken using conventional milking techniques.
Total plate count dalam isi telur ayam ras di pasar tradisional Kecamatan Waru, Kabupaten Sidoarjo Budiarto, Budiarto; Kirana, Sabila Citra; Lestari, Tita Damayanti; Budiarto; Puspitasari, Yulianna; Mutamsari Witaningrum, Adiana; Permatasari, Dian Ayu
Current Biomedicine Vol. 1 No. 2 (2023): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.1.2.86-94

Abstract

Background: Microbial contamination in consumed chicken eggs can endanger human health. Bacteria can grow and develop in food so that it can reduce the food quality. Determination of food quality can be done by various methods, one of which is Total Plate Count (TPC). Objective: The aim of this study was to determine the level of bacterial contamination in chicken eggs sold at traditional markets in Waru District, Sidoarjo Regency. Methods: Thirty samples were collected from two traditional markets, Waru Market and Wadung Asri Market. This study used the pour plate method of TPC test. Egg contents were diluted and then inoculated in Nutrient Agar. The media inoculated with the sample suspension was incubated at 37°C for 24 hours. The growing bacterial colonies were counted and analyzed using the Standard Plate Count. This study's data are presented descriptively and compared with the Indonesian National Standard (SNI) 7388-2009 maximum requirement of contaminant bacteria in chicken eggs is 1×105 CFU/mL. Results: The results showed that the averages TPC in two traditional markets were 2.1×104 CFU/mL in Waru Market and 3.4×103 CFU/mL in Wadung Asri Market. The average TPC still meets the maximum limit of microbial contamination listed in SNI 7388-2009. Conclusion: The average values of TPC in both markets are not the same, but the qualities of chicken eggs sold in Waru Market and Wadung Asri Market are good and suitable for consumption.
Prevalence and Degree Trematoda Infection in Dairy Cattle Friesian Holstein at Koperasi Usaha Tani Ternak Suka Makmur Pasuruan Rohmah, Miftahur; Sunarso, Agus; Hermadi, Herry Agoes; Hastutiek, Poedji; Permatasari, Dian Ayu; Kusnoto, Kusnoto
Journal of Parasite Science Vol. 8 No. 2 (2024): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v8i2.60450

Abstract

Trematode worm infections in dairy cattle have not been widely reported in various regions in Indonesia. This study aims to determine the prevalence and degree of trematode worm infection in Friesian Holstein dairy cattle at KUTT Suka Makmur, Pasuruan. The research design used survey study and cross-sectional study. The study was conducted in July-September 2023. The number of samples taken was 100, which were selected from proportional random sampling of four sub-districts, namely Grati, Nguling, Lekok, and Lumbang, with the age ranges under a year, 1-2 years, and above 2 years. The sample were examined using the sedimentation method and Flukenfinder® method. Positive samples were further examined using the McMaster method to determine the number of worm eggs per gram of feces. The results of this study obtained a prevalence of 54% with low and moderate degrees of infection. The trematode worm species found through fecal examination were Fasciola gigantica and Paramphistomum cervi. The results of chi square statistical analysis showed that sample examination method and age were related to the prevalence of trematode worms, yet location was not related to the prevalence of trematode worms. The results of kruskal wallis statistical analysis showed that age and location influence the degree of trematode worm infection.
Identification, Prevalence, and Degree of Digestive Tract Protozoa Infection in Dairy Cows at KUTT Suka Makmur Grati Pasuruan Margaretha, Josephira Intan; Hastutiek, Poedji; Permatasari, Dian Ayu; Sunarso, Agus; Rahardjo, Dadik; Suwanti, Lucia Tri
Journal of Parasite Science Vol. 8 No. 2 (2024): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v8i2.60454

Abstract

The aim of this research is to identify, determine prevalence, and degree infection of protozoa gastrointestinal tract in dairy cow at KUTT Suka Makmur, Pasuruan district. The research was conducted from July until September 2023 by taking 100 dairy cow faeces sample from 4 villages of KUTT Suka Makmur. The samples were examined by sedimentation and floating methods. Species are identified by looking at morphology of protozoa gastrointestinal. There were of 85 (85%) samples positively infected with protozoan. There were 3 species that infect the digestive tract, those are Eimeria sp., Balantidium sp., and Blastocystis sp. The highest infection of protozoa in this research was Balantidium sp. (54%) followed by Eimeria sp. (47%). In this study, we found that 54 samples (54%) infected by one kind of protozoa and 31 samples (31%) infected by mixed protozoa. The result of Chi Square showed no significant difference in the age on the prevalence of digestive tract protozoa. The result of Kruskal Wallis showed no significant difference in the age and degree infection of Eimeria sp.
Prevalence and Infection’s Degree of Gastrointestinal Nematode Worm in Friesian Holstein Dairy Cows at KUTT Suka Makmur Pasuruan Regency Utami, Kinanti Putri; Santoso, Kuncoro Puguh; Suwanti, Lucia Tri; Hastutiek, Poedji; Permatasari, Dian Ayu; Sunarso, Agus
Journal of Parasite Science Vol. 8 No. 2 (2024): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v8i2.60504

Abstract

This study aims to determine the type, prevalence, and infection’s degree of gastrointestinal Nematode worm in Friesian Holstein Dairy Cows at KUTT Suka Makmur, Pasuruan Regency. The study was conducted in July – September 2023. One hundred of feces samples were collected from four districts at KUTT Suka Makmur working area. Samples were examined by sedimentation and floating methods. Positive samples were further examined by McMaster methods to determine the number of worm eggs per gram feces. The type of worms were Oesophagostomum sp. (24%), Haemonchus sp. (20%), Mecistocirrus sp. (13%), Trichuris sp. (8%), Ostertagia sp. (6%), Trichostrongylus sp. (6%), Nematodirus sp. (3%), and Strongyloides sp. (3%). The prevalence of gastrointestinal Nematode worm was 66% with mild and severe degrees of infection. The Chi Square test results showed that age had no effect on the prevalence and infection’s degree of gastrointestinal Nematode worm in Friesian Holstein dairy cows at KUTT Suka Makmur. While location had effect on the prevalence but had no effect on the infection’s degree.
Bruceshield: Internet of things integrated biometric and food detection system to eradicate brucellosis milk contamination Tirtono, Jasa Dwi; Prashanti, Lupita; Saputra, Raphael Abel; Suputra, I Gede Wahyudi; Permatasari, Dian Ayu
Social Agriculture, Food System, and Environmental Sustainability Vol. 2 No. 1: (February) 2025
Publisher : Institute for Advanced Science, Social, and Sustainable Future (IASSSF)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61511/safses.v2i1.2025.1713

Abstract

Background: Brucellosis is a zoonotic infectious disease caused by the bacteria of the genus Brucella spp. that causes abortion and chronic disease in animals and humans, resulting in economic losses worldwide. Brucellosis remains endemic in ruminant livestock in Indonesia, with a prevalence rate of 40% in ruminants and the highest incidence of cases is in Java Island. Vaccination, livestock movement monitoring, and diagnostic methods such as PCR can prevent this disease, but those methods are challenged by cost and a lack of trained personnel. Methods: The methodology used in this article is a literature review. Design ideas were identified from various international and national journal literature with the main focus on test methods consisting of Multiple Cross Displacement Amplification - Lateral Flow Biosensor (MCDA-LFB) test, Retinal Biometric system, Static QR-Code system, and Internet of Things (IoT). Findings: Bruceshield is a complete entity combining animal retinal biometrics, food detection using MCDA-LFB technology, and IoT for accurate identification, rapid detection, and effective data collection of IoT-ready devices. Some of these methods include MCDA-LFB for DNA analysis, retinal biometric systems for animal identification, and static QR codes that report parasite detection and enable traceability to the consumer in dairy products. Conclusion: Bruceshield presents an innovative solution to support the vision of 'Brucellosis Free Indonesia 2025' by integrating advanced diagnostic and monitoring systems, contributing to Sustainable Development Goal (SDG) number 3 and the One Health paradigm. This system holds the potential to enhance disease prevention, improve livestock traceability, and promote transparency and safety within the dairy and livestock industry. Novelty/Originality of this article: This study introduces Bruceshield, an integrated system combining retinal biometrics, MCDA-LFB technology, and IoT for accurate livestock identification, rapid disease detection, and effective data collection. 
IMPROVEMENT DAIRY CATTLE BARN MANAGEMENT AFTER FOOT AND MOUTH DISEASE (FMD) OUTBREAK IN MEDOWO, KEDIRI Permatasari, Dian Ayu; Raharjo, Dadik; Effendi, Mustofa Helmi; Budiarto, Budiarto
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 9 No. 2 (2025): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i2.2025.260-270

Abstract

The Foot and Mouth Disease (FMD) outbreak that began in 2022 caused significant losses for dairy farmers in Medowo Village, Kandangan District, Kediri Regency—impacting both livestock health and farmers' economic stability. To address these impacts, a community service program was carried out to improve farmers' knowledge and skills in dairy cattle housing management and biosecurity implementation. The program included training sessions, educational outreach, and technical assistance for 31 farmers from KUD Kertajaya. Evaluation was conducted through pre-test and post-test instruments to measure the effectiveness of the intervention. Pre-test results showed that only 6.5% of participants were in the "good–very good" category in terms of understanding FMD and livestock management. After the intervention, post-test results showed a significant increase, with 87% of participants reaching the "good–very good" level. Additionally, in terms of practical application of cattle management practices, 100% of participants demonstrated excellent understanding after the program. This intervention proved that an educational approach combining outreach and hands-on practice can significantly enhance farmers' awareness and skills. The improvement is expected to contribute to better livestock productivity and more effective disease control. The activity also involved undergraduate students as part of the Merdeka Belajar Kampus Merdeka (MBKM) program, fostering collaboration between academia and the farming community to strengthen local capacity.
Efektivitas Model Problem Based Learning dan Make A Match dalam Pembelajaran Tematik Kelas V SD Negeri 2 Gondang Permatasari, Dian Ayu
Malih Peddas (Majalah Ilmiah Pendidikan Dasar) Vol 10, No 1 (2020): Malih Peddas, Volume 10, Nomor 1
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/malihpeddas.v10i1.5259

Abstract

AbstractAbstract: Problem-Based Learning Model and Matching in Thematic Learning at fifth grade students of 3 SD Negeri Bugangan 01 and SD Negeri 2 Gondang viewed from the appearance of critical thinking. The subjects in this study were fifth grade students at SD Negeri Bugangan 1  Elementary School as an experimental class and SD Negeri 2 Gondang Elementary School as a control class. This type of research is a quasi-experimental research type Test-Posttest Control Group Design. The Samples are 72 students. The results showed that the use of Problem Based Learning model can enhance learning about critical problems in Thematic subjects in fifth grade stdents at of 3 SD Negeri Bugangan 01 and SD Negeri 2 Gondang. It can be prove from what is supported by student’s critical thinking from the experimental class 74.21 (quite critical) and the control class 60.82 (uncritical). From the Ancova test on the calculation of Indonesian Language 1,570 level of significance or probability is 0.214. Therefore the probability is 0.214. Because of the probability 0.214 0.05, Ho is accepted, and Ha is rejected. While on the charge of Natural Sciences F count 0.334 level of significance or probability 0.565, then Ho is accepted and Ha is rejected. Related to the effective Problem Based Learning model in the Make A Match model in terms of Thematic critical thinking in fifth grade students of of 3 SD Negeri Bugangan 01 and SD Negeri 2 Gondang..Keywords: Effectiveness,Problem Based Learning and Make a Match Learning Model, identifying the type of work
Efisiensi Biaya Produksi Usaha Itik Petelur di Candi Sidoarjo Permatasari, Dian Ayu
Media Kedokteran Hewan Vol. 32 No. 2 (2021): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v32i2.2021.79-89

Abstract

Peran sub sektor peternakan terhadap pembangunan pertanian cukup signifikan, dimana industri perunggasan merupakan pemicu utama perkembangan usaha di sub sektor peternakan. Permintaan pangan hewani asal ternak (daging, telur dan susu) dari waktu ke waktu cenderung meningkat sejalan dengan pertambahan jumlah penduduk, pendapatan, kesadaran gizi, dan perbaikan tingkat pendidikan. Terdapat dua cara pengelolaan produksi telur, yakni produksi telur olahan dan tanpa olahan. Penelitian bertujuan untuk mengetahui analisis kelayakan usaha peternakan itik petelur produksi telur olahan dan tanpa olahan dan analisis efisiensi biaya produksi telur itik olahan dan tanpa olahan. Indikator analisis kelayakan usaha yaitu BEP unit, BEP harga, B/C ratio, ROI dan Payback Period. Hasil penelitian menunjukkan bahwa usaha peternakan itik petelur produksi telur olahan di Candi Sidoarjo merupakan usaha yang dapat dikategorikan layak untuk dilaksanakan dengan nilai rata-rata BEP unit 123.056 butir, rata-rata nilai BEP harga Rp 1.505,-, rata-rata nilai B/C Ratio 0,78, rata-rata nilai Return On Investment 73%, rata-rata nilai Payback Period1,90. Hasil perhitungan nilai efisiensi biaya produksi telur itik olahan menunjukkan bahwa yang paling efisien adalah responden dengan populasi 500 ekor, produksi telur 350 butir per hari atau 105.000 butir per periode dengan efisiensi biaya produksi 49%.