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All Journal Biospecies Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Bioscience Prosiding Seminar Biologi Tadris: Jurnal keguruan dan Ilmu Tarbiyah Jurnal Ilmiah Mahasiswa FKIP Prodi Biologi Jurnal Edu Research PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Biosense
Filza Yulina Ade
Program Studi Pendidikan Biologi FKIP Universitas Pasir Pengaraian, Rokan Hulu, Riau
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences

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Journal : Jurnal Biosense

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ISOLASI RIZOSFER TANAMAN NILAM SEBAGAI AGEN HAYATI TERHADAP Fusarium sp. SECARA IN VITRO Nisa, Audria Chairun; Ade, Filza Yulina; Lutfia, Anisa
JURNAL BIOSENSE Vol 9 No 1 (2026): Edisi Januari 2026
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v9i1.7223

Abstract

This study aimed to isolate Pseudomonas spp. bacteria from the rhizosphere of patchouli plants (Pogostemon cablin Benth.) at three different location, namely Aceh, Purwokerto, and Pasaman, to test their antagonistic ability in suppressing the growth of Fusarium sp., the fungal pathogen causing root rot in patcouli. Isolation using Pseudomonas Isolation Agar (PIA) medium yielded 40 rhizosphere bacterial isolates, consisting of 14 from Aceh, 16 from Purwokerto, and 10 From Pasaman. Macroscopic morphological identification revealed variations in colony characteristics such as cream/clear color, entire/undulate margins, convex elevation, and viscous/butyrous texture. In vitro antagonism tests via dual culture method on PDA medium were observe twice after 7 and 14 days of incubation, showing strong percentage inhibition of radial growth (PIRG >40%) on day 7 (PW1(2): 42,86%, PW1(3): 65,71%, PW2(3): 57,14%, PW3(1): 54,29%, PW5(2): 48,57% and on day 14 (A1(2): 65%, A2(2): 42,5%, A3(1): 47,5%, PW1(3): 50%, PW2(1): 60%, PW2(3): 55%, PW2(4): 52,5%, PW3(1): 62,5%, PW4(2): 42,5%, PW5(2): 52,5%).
Co-Authors Ali Akbar Arief Anthonius Purnama Arif Rahmad Setiadi, Arif Rahmad Dahlia - Devi Nurusdianita Sari, Devi Nurusdianita Elfi Khairina, Elfi Elmi Roza, Elmi Enny Afniyanti, Enny Estri Laras Arumingtyas Eti Meirina Brahmana Fadilaturahmah Fadilaturahmah hartati - Hilwan Yuda Teruna Jismi Mubarrak Joni, Rahma Jurati -, Jurati Kardiman, Reki Khairani - Khumaira, Kayla Anaya Lisma Yanti, Lisma Lita Dwi Handayani, Lita Dwi Luchman Hakim Lutfia, Anisa Maria Bakri, Maria Marta Dinata Martala Sari Mei Dwi Marwanti, Mei Dwi Minta Ito Hasibuan, Minta Ito Moralita Chatri Muhammad Afif Nisa, Audria Chairun Piki Lestari, Piki Pratama, Sandi Fransisco Rahmi Kurniati Realita -, Realita Rena Lestari, Rena Riki Riharji Lubis, Riki Riharji Rima Herpina, Rima Rodiyati Azrianingsih Rofiza Yolanda, Rofiza Satria, Rijal Sohibun -, Sohibun Sulaeman, Nabila Wawan Sujarwo