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Mukelar Amir
Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

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Journal : Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)

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SCAR (Sequence Characterized Amplified Region) Analysis for Pi-b and Pi-ta genes on 28 Genotypes of Rice Sobir, ,; Santoso, ,; Aswidinnoor, Hajrial; Amir, Mukelar
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 33 No. 1 (2005): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (435.578 KB) | DOI: 10.24831/jai.v33i1.1508

Abstract

Evaluation to blast disease (Pyricularia grisea) resistance was carried out by using two SCAR (Sequence Characterized Amplified Region) markers of Pi-b and Pi-ta blast resistance genes, and spray-inoculation method with 10 races of P. grisea on 28 paddy genotypes, that consisted six wild genotypes of rice. The results revealed that among 28 paddy genotypes, fifteen genotypes carrying both genes including Oryza rufipogon; six genotypes carrying Pi-b genes including O. alta, two genotypes carrying Pi-ta gene, and five genotypes did not possess both gene including O. glumaepatula, O. officinalis, O. latifolia, and O. malapuzhaensis. Based on infection intensity, the evaluated genotypes were vary in responses to different  ten races of P. grisea, indicated that the evaluated genotypes were  vary in carrying Pi genes. Analysis in detail indicated that existence of Pi-ta gene associated with lower infection intensity caused by P. grisea race 063 C.   Keywords:  SCAR markers, rice blast resistance, Pyricularia grisea
SCAR (Sequence Characterized Amplified Region) Analysis for Blast Resistant Evaluation on 12 Genotypes of Rice Sobir, ,; Andrianyta, Harmi; Amir, Mukelar
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 31 No. 1 (2003): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (76.829 KB) | DOI: 10.24831/jai.v31i1.1526

Abstract

Resistance evaluation to blast disease (Pyricularia grisea) on 12 paddy genotypes was carried out in the green house by using spray inoculated method with race 033 and 041 of P. grisea , and SCAR (Sequence Characterized Amplified Region) marker by using Pib primer pairs. The results revealed that among 12 paddy genotypes were classified into six resistance groups. The first group comprised two genotypes (Jatiluhur and Asahan) having three resistance genes. The second group comprised two genotypes (Oryza malampuzhaensis and O. punctata) having two resistance genes against race 033 and 041. The third group had one resistance gene against race 033, comprised one genotype (Way Rarem). The fourth group comprised one genotype (Danau Tempe) having two resistance genes against 041 race and Pib. The fifth group comprised three genotypes (Kalimutu, Maninjau and Laut Tawar) having two resistance genes against race 033 and Pib. The sixth group comprised two genotypes (Kencana Bali and Cirata) having no resistance gene to blast race 033 and 041, and Pib.  These results indicated that Pib gene did not confer resistance to race 033 and 041 of Pyricularia grisea.  Resistance to race 033 and 041 might be controlled by different resistant gene.   Key words : SCAR, Blast resistant, Rice
Co-Authors , Santoso HAJRIAL ASWIDINNOOR Harmi Andrianyta Sobir Sobir