This study aims to examine and analyze the role of organizational culture in moderating the influence of emotional intelligence and professional competence on teacher performance at SMKN 1 Kedungwuni, Pekalongan Regency. This study with a population of 101 people, namely all teachers of SMKN 1 Kedungwuni, Pekalongan Regency. The data collection technique used survey methods and the research instrument used a questionnaire with a Likert scale 5. The instrument test was carried out with validity tests through CFA, reliability tests, normality tests, heteroscedasticity tests, and model tests (F test, R-Square and sig). Test the hypothesis with quasi-regression analysis, interaction technique by looking at the sig value. The results showed that emotional intelligence had a positive effect on teacher performance, professional competence had a negative and significant effect on teacher performance, organizational culture had no significant effect on teacher performance, organizational culture moderated the influence of emotional intelligence and professional competence on teacher performance