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All Journal Medula JK Unila (Jurnal Kedokteran Universitas Lampung)

Indria Hafizah

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Author-ID : 2865636

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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UJI AKTIVITAS ANTIBAKTERI EKSTRAK METANOL RUMPUT LAUT (Eucheuma sp) PADA BERBAGAI TINGKAT KONSENTRASI TERHADAP PERTUMBUHAN BAKTERI Escherichia coli DAN Staphylococcus aureus Indria Hafizah; Nur Illiyin Akib; Muhammad Fajrianto
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 1, No 2 (2014)
Publisher : Halu Oleo University

Red algae seaweed Eucheuma sp containflavonoids compound which has activity as anantibacterial. Thisstudyaimstodeterminethe difference of seaweed methanol extract(Eucheuma sp) on inhibiting the growth ofEscherichia coli andStaphylococcus aureusbacteria. This research was conducted with post–test only design (one-shot case study) with a variable treatment of theseaweed methanolextract (Eucheuma sp) against S. aureus and E. coli.Extractswerethendividedinto 5 concentration of 75%, 50%, 25%, 5%, and non-extraction (squeezed). Analysis of data t odetermine the level of concentration differences seen from the ANOVA test (analysis of variance) followedby a posthoc test. The results of bivariat analysis showed the inhibition of E. coli bacteria there are difference in clear zone diameter which are significant various levels of each concentration, with a value of p = 0.000(p < 0.05) and in S. Aureus inhibition P = 0.073 (p > 0.05) which means there is no difference in the diameter of clear zone at various levels for each concentration. The conclusions of this research are differences extract concentration have effect on the inhibition of the growth of the bacterium Escherichia coli and the difference in concentration of the extract have no effect on the inhibition of growth of Staphylococcus aureus.Key words : Eucheuma sp, Escherechia coli, Staphylococcus aureus

Uji Daya Hambat Ekstrak Etanol Bintang Laut Bertanduk (Protoreaster nodosus) terhadap Bakteri Streptococcus sp. dan Candida albicans Indria Hafizah; Sulastrianah Sulastrianah
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 3, No 1 (2015)
Publisher : Halu Oleo University

Starfish is a marine biota that have potency for used in human health. Protoreaster nodosus is species of starfish that commonly found in water of southeast Sulawesi and not widely used by local community. The aim of this study is to find out bioactive compound and to examine the antibacterial and antifungal bioactivity ethanol exract of Protoreaster nodosus. Thin Layer Chromatography (TLC) is used in bioactive compound assay and disk diffussion method is used to examine antibacterial and antifungal activity. Ethanol extract of Protoreaster nodosus is diluted into 2000 ppm, 4000 ppm, 8000 ppm, 16.000ppm and 32.000 ppm. As the result result of this study, ethanol extract of Protoreaster nodosus are positively containing alkaloid, steroid, saponin, flavonoid and tannin. The diameter of clear zone of disk containing extract in nutrient agar with Streptococcus sp are 4,4 mm, 5,4 mm, 6,7 mm, 9,4 mm dan 11,4 mm, consecutively. The diameter of clear zone of disk containing extract in potato dextrose agar with Candida albicans are 6,6 mm, 9,15 mm, 11,5 mm, 13,95 mm and 20,7 mm, consecutively. In the conclusion, ethanol extract of Protoreaster nodosus from Southeast Sulawesi containing alkaloid, steroid, saponin, flavonoid and tannin. The Antibacterial activity is strong in concentration of 32.000 ppm and the antifungal activity is strong in 32.000 ppm, 16.000 ppm, 8.000 ppm, 4000 ppm concentration. Keywords: Protoreaster nodosus, antibacterial activity, antifungal activity Streptococcus sp, Candida albicans, secondary metabolite

UJI DAYA HAMBAT EKSTRAK DAUN TAPAK KUDA (Ipomoea pes caprae (L) R. Br. ) TERHADAP Staphylococcus aureus Alminsyah Alminsyah; Indria Hafizah; Sulastrianah Sulastrianah
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 2, No 1 (2014)
Publisher : Halu Oleo University

Ipomoea pes caprae (L) R. Br are commonly used by the Indonesian’s people to treat wounds, ulcers and as antioxidant. Based on previous research it was contains terpenoids, saponins, alkaloids, tannins and flavonoids. The aim of this study is to determine inhibition capacity of I. pes caprae (L) R. Br leaf extract toward Staphylococcus aureus isolated from patients infected wounds at a concentration of 100 % , 50 % , 25 % , 12.5 % , 6.25 % , 3.125 % and 1.56 %. This research was conducted with post-test design with a control only. Erythromycin is used as a control. One gram of extract dissolved in 10 ml of chloroform. Inhibition zone is measured by paper disc diffusion method on Mueller Hinton agar. Bivariat analysis showed that there is statistically significant differences of diameters of inhibition zone from each concentration (p=0,00) including control (p=0,00). Conclusion of this study are I. pes caprae (L) R. Br leaf chloroform extract have capacity to inhibit S. aureus growth and the inhibition respond increase with concentration. Keywords : Ipomoea pes caprae (L) R. Br, Inhibition test, Erythromycin, Staphylococcus aureus

Aktivitas Antibakteri Ekstrak Etanol Porifera (Spongia Officinalis ) terhadap Staphylococcus Aureus ATCC 25923 Indria Hafizah; Fine Farhani Muliati; Sulastrianah Sulastrianah
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 4, No 1 (2016)
Publisher : Halu Oleo University

Staphylococcus aureus is the most dangerous of all of the many comman staphylococcal bacteria. It stains gram positive and is non moving small round shaped or non motile cocci..Porifera (Spongia officinalis) are lowly organised less evelved animals and can be used as the medication resources. The aim of this study was to inveftigate antibacterial extract etanol of Porifera (Spongia officinalis)against the growth of Staphylococcus aureus ATCC 25923.Present study was an experimental in vitro test. Antibacterial activity assays were carried out by the disc diffusion method. Antibacterial activity was determined by the clear zone formed around the papaer disc and minimum inhibitor concentration which could inhibit the growth of Staphylococcus aureus ATCC 25923.The results are supported by the presence of secondary metabolite by phytochemical test such as alkaloid, terpenoid, steroid, saponin and tanin.Extract ethanolof Spongia officinalis. Extract etanol Spongia officinalishas antibacterial activity againts Staphylococcus aureus ATCC 25923. Minimum inhibitory concentration from the ekstrak etanol Spongia officinalis was 10 ppm.Conclution ; Extract etanol Spongia officinalishas antibacterial activity againts Staphylococcus aureus ATCC 25923Keywords: Spongia officinalis, Staphylococcus aureus, Antibacterial activities, MIC (Minimal Inhibitory Consentration)

Bioaktifitas Antibakteri Ekstrak Etanol Teripang Pasir (Holothuria scabra) terhadap Pertumbuhan Klebsiella pneumoniae Secara In Vitro Fifit Ervitha; Sulastrianah Sulastrianah; Indria Hafizah
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 3, No 1 (2015)
Publisher : Halu Oleo University

Holothuria scabra is one of the biota that commonly found in Southeast Sulawesi waters and traditionally use as a source food and medicine by local tribe. They also harvesting this species for exported to several countrywith high demand. Even this species have commmercially beneficience, but there is lack of publication about the bioactivity, especially H. Scabra derived from southeast Sulawesi. This study aims to determine secunder metabolite that contain in the extractand to examine antibacterial bioactivity of H. scabra ethanol extract againstKlebsiellapneumoniae by defining the Minimum Inhibitory Consentration. The method using an experimental with post test onlycontrol group design. The extraction is using maseration method and the extract then diluted and divided intoten concentration 100%, 50%, 25%, 12,5%, 6,25%, 3,125%, 1,56%, 0,78%, 0,39%, and 0,19%. The secunder metabolite was determined by using TLC and the MIC was determined by using dilution method. All consentration.As the result, TLC test showing positif result for alcaloid and triterpenoid and the nutrient broth starting to show no turbidity in concetration 0,78%. The conclusion of this study is ethanol extract of H. Scabra contain alkaloid and triterpenoid as secunder metabolite and the MIC was in 0,78% concentration.Keywords: Holothuria scabra, Klebsiella pneumoniae, MIC, alkaloid, terpenoid

Co-Authors Alminsyah Alminsyah Cici Hudfaizah Fifit Ervitha Fine Farhani Muliati Juminten Saimin Muhammad Fajrianto Nur Illiyin Akib Sulastrianah Sulastrianah, Sulastrianah

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