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All Journal Buletin Peternakan
Pudji Astuti
Department of Physiology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
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Journal : buletin peternakan

 1 
The Adaptation of Small Intestine Nitregic Myenteric Neurons on Rats (Rattus norvegicus) to High Fat Diet Amelia Hana; Sarmin Sarmin; Claude Mona Airin; Christin Marganingsih Santosa; Pudji Astuti
Buletin Peternakan Vol 43, No 2 (2019): BULETIN PETERNAKAN VOL. 43 (2) MAY 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i2.38810

Abstract

High fat diet can result in the loss of nitrergic neurons in the myenteric plexus. The study aimed at finding out the effect of high fat diet on the adaptation of nitrergic nerve of rat intestine. It used 15 male rats (Rattus norvegicus) of a month of age with mean body weight of 53.73 gr. The rats were adapted for 7 days to individual cages with ad libitum feeding. After random adaptation, all of the rats were assigned to 3 groups of five rats, namely K-7, K-10, and K-13 groups. Feed and drinking water were given ad libitum. The treatment of the high fat diet lasted for 7 weeks. After the treatment, all of the rats were fasted for 12 hours and then killed. Subsequently, small intestine segments (duodenum, jejunum, and ileum) were taken. The intestinal segments were prepared by using NADPH-d histopathological technique to determine the morphometric changes of nitrergic myenteric neurons. During the treatment the rats were weighed every week and at the end of the study orbitalis vein blood measurement was carried out to see its glucose, cholesterol and cholecystokinin (CCK) plasma levels. The data of body weight, glucose, cholesterol, CCK levels, the total number of the nippergenic myenteric segments of the small intestine were statistically analyzed using Anova. The results of the study showed that the treatment of 7% to 13% fat diets for 7 weeks did not indicate any weight gain and any increase in cholescystokinin level, and any decrease in glucose level. However, it indicated significant increase in cholesterol level. The treatment of 10% and13% fat diets increased the total number of neurons in the jejunum and the ileum. Thus, it was concluded that the treatments of the high-fat diet of the rats (K-7, K-10, and K-13) for 7 weeks had significant effect on the adaptation of the neurons of the jejunum and the ileum.
The Hematological and Blood Chemical Parameters of the Female Fat Tail Sheep Raised with Fermented Complete Feed Management Sarmin Sarmin; Amelia Hana; Pudji Astuti; Claude Mona Airin
Buletin Peternakan Vol 44, No 4 (2020): BULETIN PETERNAKAN VOL. 44 (4) NOVEMBER 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i4.54669

Abstract

The study aimed at evaluating the hematological and chemical parameters of the blood of female fat tail sheep raised with fermented complete feed (CF) management. It used 40 adult female fat tail sheep age 24-36 months and body weight in 30-35 kg and 3-4 of body condition score, which was raised in the individual stall with fermented complete feed (CF) management. Samples of blood were drawn once from the jugular vein before feeding in the morning. The results of hematological parameters were RBC (12.11±2.18 x106/µL), hemoglobin (12.40±1.96 g/dL), hematocrit  (36.49±5.47%), MCV (30.46±3.38  fL), MCH (10.30±0.75 pg), MCHC (34.00±1.86 g/dL), eosinophils (3.15±7.70%), neutrophils (7.13±14.90%), lymphocytes (11.93±23.85%), and monocytes (0.30±0.88%). The results of chemical parameters were sodium (147.58±3.75 mmol/L), chloride (106.51±3.12 mmol/L), magnesium (3.66±1.38 mg/dL), albumin (3.36±0.31 g/dL) and glucose (40.28±9.12 mg/dL). The results of hematological and chemical parameters in the study could be the reference in monitoring the health of the adult female fat tail sheep raised with the fermented complete feed (CF) management.
The Hematological and Biochemical Profiles of Wonosobo Sheep Blood in Various Physiological Conditions Sarmin Sarmin Sarmin; Pudji Astuti; Claude Mona Airin
Buletin Peternakan Vol 46, No 3 (2022): BULETIN PETERNAKAN VOL. 46 (3) AUGUST 2022
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v46i3.73423

Abstract

The study aimed at examining the hematological and biochemical profiles of Wonosobo sheep blood in various physiological conditions. There were 32 Wonosobo sheep in the various physiological conditions, including 17 ewe lamb of 1-2 months of age, 5 pregnant female sheep of 12-48 months of age at 3 months of pregnancy, 5 ewe of 12-48 months of age in second lactation period of 1-2 months and 5 ram of 12-48 months of age. Each of the sheep was put in individual sheepfold and given Pakchong-1 grass, dried kangkong, and concentrate. Drinking water was given ad libitum. Blood sample was drawn from jugular vein and put into tubes for blood hematological and biochemical analyses. The results of the study showed that total leukocyte and lymphocyte were high in young sheep, while neutrophils was high in pregnant female sheep (P<0.05). There was not any significant physiological impact on hemoglobin, MCV, MCH, MCHC, RDW, hematocrit, platelet, monocyte, eusinophil, and basophil (P>0.05). The highest ALP activity and Pi levels were found in the ewe lamb, while low creatinine was found in the ram (P<0.05). The parameter of the activities of SGPT, SGOT, LDH, calcium, kalium, chloride, sodium, magnesium, TIBC, UIBC, TS, globulin, albumin, total protein, BUN, creatinine, glucose, cholesterol, triglyceride, HDL and LDL was not affected by physiological conditions (P>0.05). It was concluded that the impact of the physiological conditions of the Wonosobo sheep caused high total leukocyte and lymphocyte in ewe lamb and high neutrophil in pregnant female sheep, the increase in the Pi level and ALP activity of the ewe lamb, and high creatinine in the ram
Co-Authors Amelia Hana Amelia Hana Christin Marganingsih Santosa Claude Mona Airin Sarmin Sarmin