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All Journal Buletin Veteriner Udayana
Hana Kristal Alamanda Septiara
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Karakteristik Fisikokimia Bakteriosin Asal Bakteri Asam Laktat Enterococcus durans Hasil Isolasi Kolon Sapi Bali I Wayan Suardana; Hana Kristal Alamanda Septiara; I Nyoman Suarsana
Buletin Veteriner Udayana Vol. 9 No. 2 Agustus 2017
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Lactic acid bacteria can excrete antimicrobial compounds like bacteriocins. The study aimed to find out the characteristic of physic-chemical of bacteriocin producing Enterococcus durans isolate 18A isolated from colon’s bali cattle. The study initiated by Gram staining and catalase test, followed by isolation and purification of bacteriocin. The result of the research showed that bacteriocins of Enterococcus durans isolate 18A as a protein with it’s concentrationis 0,272?g/ml and it does not contain carbohydrate. On the other hand, the bacteriocins was not showed a band while tested on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The result showed, the bacteriocin producing Enterococcus durans isolate 18A has antimicrobial activity to Bacillus cereus as 23,88%.
Co-Authors I Nyoman Suarsana I Wayan Suardana