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Anna Nabilla
Program Studi Biologi Reproduksi, Sekolah Pascasarjana, Institut Pertanian Bogor, Laboratorium Prossesing Semen Sapi Bali, UPT Balai Inseminasi Buatan Daerah (BIBD) Baturiti. Jalan Raya Bedugul Km 43, Baturiti, PO. BOX 82191, Tabanan, Bali, Indonesia
Veterinary

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KUALITAS SEMEN SEGAR SAPI BALI PADA DUA KELOMPOK UMUR DAN PENENTUAN KONSENTRASI KRIOPROTEKTAN DALAM PENGENCER TRIS KUNING TELUR (Fresh Semen Quality Of Bali Bull In Two Group Of Age And Determination Of Cryoprotective Agent Concentration In Tris Eggyolk Extender) Anna Nabilla; Raden Iis Arifiantini; Bambang Purwantara
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.162 KB) | DOI: 10.19087/jveteriner.2018.19.2.242

Abstract

The aim of this study was to compare the quality of Bali bull fresh semen in productive group (below 10 years old) with over productive group and to evaluate the frozen semen quality of bali bull after cryopreservation in Tris egg yolk extender using Dimethyl formamide (DMF) and glycerol in three different concentrations (5%, 6%, and 7%). Semen was collected using artificial vagina. Semen then evaluated macro and microscopically. Macroscopic evaluation conducted visually and microscopic evaluation with the aid of Computer Assisted Sperm Analysis CASA. Immediatelly after evaluation, semen were divided into six aliquots and diluted with Tris egg yolk (TEY) glycerol 5% (TEYG5), TEY-glycerol 6% (TEYG6), TEY glycerol 7% (TEYG7), TEY-DMF 5% (TKTD5), TEY-DMF 6% (TEYD6) and TEY-DMF 7% (TKTD7). Results of experiment showed that there was no difference (P>0.05) among the all parameters, except for semen volume. Semen volume of productive group was higher than over productive age group. No difference was found in post thawing motility of the spermatozoa in TEY extender with 5% glycerol, and TEY with 5%, 6% and 7% DMF. The higher recovery rate of bali bull spermatozoa demonstrated by spermatozoa in TEY extender with glycerol 5% and DMF 6% (63.33±2.40 dan 68.67±2.33). It was concluded that bali bull semen quality did not differ between productive and over productive ages and post thawing semen quality of bali bull in TEY with 5-7% DMF or 5% glycerol was not also different.
Co-Authors Bambang Purwantara R. Iis Arifiantini