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All Journal Prosiding Seminar Biologi Prosiding KPSDA
Riris L. Puspitasari
Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Al-Azhar Indonesia, Jakarta
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Pleurotus ostreatus sebagai Nutrisi Pertumbuhan pada Mus musculus Puspitasari, Riris L.
Prosiding KPSDA Vol 1, No 1 (2015): Prosiding KPSDA 2015
Publisher : Prosiding KPSDA

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Abstract

Pleurotus ostreatus or white oyster mushroom had been produced commercially on an industrial scale as food and supplements. It is because of this oyster mushroom contained nutritions that were nutriceutical benefit. These fungi acted as anti-tumor, lower cholesterol and anti-oxidants. Based on the wealth of information on the nutritions and the many benefits of this white oyster mushroom, we run the research to obtain information related to the potent of oyster mushrooms in mice. So that white oyster mushroom can be used as a potent medicine to heal various diseases. The purpose of this study was to determine the potency of white oyster mushroom as nutrition growth in mice. This study began with the optimization of the white oyster flour making. Mice in dose of 100 mg / KgBW showed the highest body weight compared to other doses. In the body length parameter indicated that the treatment group with dose of 100 mg / KgBW gave an average body longest compared to other groups. This dose also rise the highest feed intake while the group with the least amount of feed intake was in dose of 200 mg / KgBW.
CONDITIONED MEDIUM DARI KULTUR PRIMER SEL SYARAF MUS MUSCULUS Puspitasari, Riris L.; Boediono, Arief; Sandra, Ferry
Prosiding Seminar Biologi Vol 10, No 2 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Abstract

Secara  in vitro,  Embryonic Stem Cell  (ESC) dapat diarahkan perkembangannya menjadi sel neuron dan sel glia. Conditionedmedium  dari kultur primer sel syaraf mengandung sejumlah faktor pertumbuhan antara lain  nerve growth factor (NGF), glial derived-neurotrophic factor (GDNF), nestin, dan glial fibrillary acidic protein (GFAP). Dengan melakukan purifikasi protein yang terkandung  di dalam CM, maka diharapkan spektrum protein yang ada menjadi lebih sempit sehingga protein target dapat terdeteksi. Penelitian ini mempelajari kultur primer sel syaraf yang berasal dari hemisfer Mus musculus. Tujuan penelitian adalah untuk mendapatkan CM dari kultur primer sel syaraf  Mus musculus. Medium yang digunakan adalah Dulbecco?s Modified Eagle?s Medium (DMEM) highglucose FBS 10%. Penggantian medium kultur dilakukan setiap 2 hari sekali. Kepadatan sel sekitar 32x103 sel/2 cm2. Setelah hari ke-4 terlihat adanya pertumbuhan neuron bipolar dan neural progenitor cell (NPC). Sel-sel astrosit akan teramati ketika periode kultur diperpanjang. Sel mengalami konfluensi setelah 12 hari kultur. Sel-sel yang tumbuh berguna untuk penjelasan neurogenesis. Kultur primer sel syaraf secara monolayer yang berasal dari hemisfer neonatus mampu mendukung  pertumbuhan sel yang tergolong sebagai neurogenic dan nonneurogenic.  Kata kunci: kultur primer, sel syaraf, conditioned medium, neural progenitor cell, neurogenesis.
Co-Authors Arief Boediono Ferry Sandra