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All Journal Indonesian Aquaculture Journal Jurnal Riset Akuakultur

Huria Marnis

Balai Penelitian Pemuliaan Ikan, Jl. Raya 2 Sukamandi-Pantura Patok Beusi, Sukamandi-Subang

Author-ID : 3672457

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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CORRELATION OF MICROSATELLITE DNA MARKERS WITH GROWTH TRAITS IN STRIPED CATFISH (Pangasianodon hypophthalmus) Huria Marnis; Evi Tahapari; Jadmiko Darmawan
Indonesian Aquaculture Journal Vol 13, No 2 (2018): (December, 2018)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Marker-assisted selection in genetic improvement of striped catfish is useful in the breeding program of the fish. Five microsatellite markers were characterized in the largest (4.03 kg ± 1.67 kg) and smallest (1.41 kg ± 0.22 kg) individuals. Five polymorphic loci were then used to genotype 160 individuals and the associations between their genotypes and growth traits were examined. The result showed that twenty-seven alleles were detected in striped catfish. The number of alleles per locus (NA) ranged from 4 to 7, with an average of 5.4 alleles per locus. The effective number of alleles per locus (NE) ranged from 3.940 to 6.939, with an average of 5.32 alleles per locus. HO and HE ranged from 0.125 to 0.944 (mean value of 0.472) and from 0.564 to 0.775 (mean value of 0.697), respectively. PIC ranged from 0.573 to 0.799 (mean value of 0.706), showing that they were highly polymorphic loci. Only one microsatellites loci (Pg13) that showed significant differences (P<0.01) in the associations between their genotypes and growth traits, while Pg3 and Pg14 were significantly associated with the standard length (P<0.01) and body weight (P<0.05). However, the Pg1 and Pg2 were not significantly associated with the body weight and standard length. Four genotypes of three loci were positively correlated with the growth traits (body weight and standard length) i.e. genotypes 194/194 for Pg3, 227/227 and 229/229 for Pg13, 279/279 for Pg14. These four genotypes can be used to identify growth traits in the molecular marker-based selection of a breeding program.

THE ABILITY OF FAST-GROWING TRANSGENIC AFRICAN CATFISH (Clarias gariepinus) ON PREDATOR AVOIDANCE Huria Marnis; Bambang Iswanto; imron imron; selny Febrida; Raden Roro Sri Pudji Sinarni Dewi
Indonesian Aquaculture Journal Vol 11, No 1 (2016): (June 2016)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Research Institute for Fish Breeding has produced transgenic African catfish (Clarias gariepinus) containing stripped catfish growth hormone gene (PccBA-PhGH) with growth 19.86% faster than that of non-transgenic fish. This fish has high potential to be released and utilized for fish farming sector to increase national production. However, there is not yet information about environmental risk of this fish. One of the major fitness traits determining potential environmental risk is predator avoidance. This study aimed to determine the predator avoidance ability of transgenic African catfish in an experimental laboratory condition. In this study, thirty five individuals each of transgenic and non-transgenic with body weight of about 0.1 ± 0.019 g were communally stocked in 60 cm x 40 cm x 40 cm aquarium with limited feeding frequency (ad libitum twice a day). One day after the fish were stocked, the predators were added to each aquarium. The non-transgenic and transgenic with body weight of 1.0 ± 0.024 g were stocked as predators as many as five individual in each aquarium. After approximately two weeks of predation, all remaining fish were collected for transgenic verification by PCR method. Genomic DNA was isolated from fin tissue of individually survivors. The results of this study showed that the transgenic fish had worse predator avoidance and lower cannibal than non-transgenic (P<0.05). There was no significant difference in growth performance between transgenic and non-transgenic African catfish (P>0.05) in limited food. The transgenic fish may have lower fitness than non-transgenic.

GENETIC PERFORMANCE OF THE STRIPED CATFISH (Pangasianodon hypophthalmus Sauvage, 1878) POPULATION DERIVED FROM SELECTIVE BREEDING Huria Marnis; Evi Tahapari; Jadmiko Darmawan
Indonesian Aquaculture Journal Vol 14, No 1 (2019): (June, 2019)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Selective breeding to improve growth trait in striped catfish (Pangasianodon hypophthalmus) need genetic performance data. This study was carried out to evaluate genetic performance of different generations of the striped catfish population derived from selective breeding. Fifty fish of each population from four generations selected striped catfish was analyzed using five microsatellite loci (Pg-1, Pg-2, Pg-3, Pg-13, and Pg-14). Microsatellite allele data were analyzed using Microsoft Excel, Arlequin, and Fstat software. A neighborhood joining dendrogram was constructed based on Nei’s distance (Da) matrix with 1,000 bootstrap replications using MEGA7 software. The result showed that totally 31.5 exist, ranged from 4-7. Number of allele was ranged from 5.0 to 5.2, and polymorphic data was from 0.45-0.60. There were some exception, such as the allele of the loci Pg-2—194 bp, Pg-13—227 bp, Pg-13—229 bp, and Pg-14—279 bp; their gene frequencies were increased by generation. Further analyses indicated that most genetic variations arise from individuals within populations (approximately 57.10%). The founder generation closely related to G-0 generation. Likewise, G-1 generation closely related to G-2 generation. This result indicated that selection activity had a very significant impact on the genetic improvement of the selected population.

Co-Authors Bambang Iswanto Evi Tahapari Evi Tahapari imron imron Jadmiko Darmawan Jadmiko Darmawan Raden Roro Sri Pudji Sinarni Dewi Raden Roro Sri Pudji Sinarni Dewi Romy Suprapto selny Febrida

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