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All Journal Jurnal Ilmu Kefarmasian Indonesia Harmoni Sosial : Jurnal Pengabdian Dan Solidaritas Masyarakat
Ikrimah Nisa Utami
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Biochemistry, Genetics & Molecular Biology Education Health Professions Medicine & Pharmacology Public Health Social Sciences Other

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Uji Banding Metode Isolasi DNA Sampel FTA Card menggunakan Kit Wizard® Genomic DNA Purification, PureLink® Genomic DNA, dan Chelex-100 Dyah Aryani Perwitasari; Imaniar Noor Faridah; Yeni Alfiana Ratnasari; Kania Agustina; Ikrimah Nisa Utami; Rita Maliza
JURNAL ILMU KEFARMASIAN INDONESIA Vol 18 No 2 (2020): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v18i2.838

Abstract

FTA Cards are an easy way to collect blood samples, deliveries, and stores. The totalvolume of blood samples is less is needed when compared to the blood samples taken through a vein(venipuncture). The purpose of this research is a method that can be applied to isolate DNA samplesfrom FTA Cards using the Wizard® Genomic DNA Purifi cation Kit (Promega), PureLink® GenomicDNA Kits, and Chelex-100. This research was a type of experimental research, and data analysis wasshowed in qualitative and quantitative descriptively. The samples used were 16 samples of human bloodtaken using a lancet and then placed on the FTA Card, sampling was done by accidental samplingtechnique. The variations of the PureLink® Genomic DNA kits method with the addition of SDS 10%showed thin and smear DNA bands on agarose gels. Furthermore, the Chelex-100 method showed aclear DNA band. The purity data obtained varied between 1 to 1.1 and concentrations between 190 to950 ng/μl. The conclusion of this study is DNA kits method with the addition of 10% SDS and theChelex-100 method still have impurities, with the purity value of DNA was less than 1.8 and a lowconcentration to be used as a DNA template for further molecular analysis.
Festival Lansia Sehat dan Mandiri: Bebas Askariasis Diah Karomah Putri; Farsida Farsida; Risya Mawahdah; Cyntya Harlyana; Farabillah Afifah; Rahma Ayu Larasati; Ikrimah Nisa Utami; Hasyasya Furnita
Harmoni Sosial : Jurnal Pengabdian dan Solidaritas Masyarakat Vol. 3 No. 1 (2026): Januari: Harmoni Sosial : Jurnal Pengabdian dan Solidaritas Masyarakat
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62383/harmoni.v3i1.2815

Abstract

The elderly are highly susceptible to ascariasis infection because, in addition to the occurrence of immunosenescence, helminths possess the ability to evade host immune responses, making them difficult to eradicate. This community service activity aimed to improve health literacy and early detection of diseases among the elderly in Pejaten, South Jakarta, with a specific focus on Ascariasis. The program was successfully attended by approximately 70 residents, where a medical team from the Faculty of Medicine and Health conducted comprehensive health history assessments and free medical check-ups. This education proved vital as a catalyst for behavioral change, shifting the community's initial perception that worm infections only affect children to an understanding that adults and the elderly are also at high risk. Through health screenings, residents also gained critical insights into their physical condition, including blood pressure, heart, lung, digestive, and skin health, as well as identifying personal risk factors for Ascariasis.
Co-Authors Cyntya Harlyana Diah Karomah Putri Dyah Aryani Perwitasari Farabillah Afifah Farsida, Farsida Hasyasya Furnita Imaniar Noor Faridah Kania Agustina Maliza, Rita Rahma Ayu Larasati Risya mawahdah Yeni Alfiana Ratnasari