Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Jurnal Veteriner
Ketut Santhia Adhy Putra
Unknown Affiliation
Veterinary

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Kloning, Sikuensing dan Analisis Filogenetik Gen Nukleokapsid Protein Virus Tetelo Isolat Bali-1/07 Anak Agung Ayu Mirah Adi; I Made Kardena; Nyoman Mantik Astawa; Ketut Santhia Adhy Putra; Yoshihiro Hayashi; Yasunobu Matsumoto
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.619 KB)

Abstract

A study was carried out on the molecular characteristics of gene encoding for nucleocapsid protein(NP) of Newcastle disease virus (NDV) Bali-1/07. A portion of the fragment gene was amplified by reversetranscriptase-polymerase chain reaction (RT-PCR). The RT-PCR product purified from the gel was treatedwith Taq polymerase and cloned into plasmid pT7blueT vector. The recombinant plasmid was tranfectedinto Nova blue singles strain of Escherichia coli-competent cells and selected using white and blue colorselection method. Good white colonies were subcultured and tested for presence of expected gene fragmentby polymerase chain reaction (PCR). The correct size PCR product was recloned and sequenced. The PCRproduct of 540 base pairs were sequenced and aligned with several cognates genes of world NDVisolates.using Cluster IW. The phylogenetic analysis was then performed using MEGA 4. Phylogeneticanalysis showed that the NP gene of NDV Bali-1/07 is closely related with virulent NDVs such asGuangxii-11/03, KBNP-4152 and Ast2755/01 with nucleotide sequence homology of 92%, 91.4% and91%respectively, whereas the nucleotide sequence homology with avirulent NDVs such as LaSota and B1were 77%.
DETECTION OF NEWCASTLE DISEASE VIRUS BY NESTED REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION Anak Agung Ayu Mirah Adi; Nyoman Mantik Astawa; Ketut Santhia Adhy Putra; Yasunobu Matsumoto
Jurnal Veteriner Vol 9 No 3 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1816.975 KB)

Abstract

A study to utilize nested reverse trancriptase-polymerase chain reaction (RT-PCR) for the detection ofNewcastle disease virus (NDV) infection was carried out. NDV isolated from an outbreak in KarangasemDistrict of Bali, Indonesia was propagated in chicken embryos and its pathogenicity was determined byinoculation in 3 week-old chickens. Organ samples were collected from infected chickens for nested-RTPCR.Out of several different pairs of primers designed for study, 3 pairs of primers (F4s-F6r/F5s-F5r,F8s-F10r/F8s-F8r and F12s-F14s/F13s-F13r), each of which for first-round/nested PCR, was able to amplifyspecific regions of NDV genome. In the fist-round PCR, the PCR product of 1500 bps was not clearly visiblein the agarose gel following electrophoresis. In nested PCR a PCR product of 500 bp was clearly visible onagarose gel following electrophoresis. The 3 pairs of primers appeared to be potential for an accurate andrapid detection NDV in the infected host.
Co-Authors DWI SURYANTO I Made Kardena I NYOMAN MANTIK ASTAWA Yasunobu Matsumoto Yoshihiro Hayashi