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All Journal Caraka Tani: Journal of Sustainable Agriculture
Ongko Cahyono
Fakultas Pertanian, Universitas Tunas Pembangunan Surakarta
Agriculture, Biological Sciences & Forestry

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STUDI KARAKTERISASI ANGGREK SECARA SITOLOGI DALAM RANGKA PELESTARIAN PLASMA NUTFAH Sri Hartati; Linayanti Darsana; Ongko Cahyono
Caraka Tani: Journal of Sustainable Agriculture Vol 29, No 1 (2014): March
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.331 KB) | DOI: 10.20961/carakatani.v29i1.13300

Abstract

Character was very important cytological nature orchids studied to support the success of breeding orchids. The purpose of the research to study the cytological characters orchid number, size) and chromosome karyotype in order to conserve germplasm. The study was conducted at the Laboratory of Biotechnology Faculty of Agriculture University Sebelas Maret Surakarta. Materials research Paphiophedilum glaucophylum, Coelogyne spesiosa, Dendrobium crumenantum, Dendrobium mutabile,, Bulbophyllum. Blumei dan Bulbophyllum beflorum. The method uses squashing methods include : fixation : Carnoy solution of 2 (6 ethanol : 3 chloroform : 1 glacial acetic acid 45 %), hydrolysis (1 N HCl) and staining chromosomes (aceto - orcein solution 2 %). Observations (number, size, shape) chromosomes with a light microscope. The results showed that the studied semuia orchids have the same chromosome number is 2n = 38, medium size chromosomes Paphiopedilum glaucophyllum 4.26 ± 0.14 to 9.73 ± 0.19, Coelogyne spesiosa 0.35 ± 0.03 to 6.30 ± 0.84, Dendrobium crumenantum 2.46 ± 0.20 until 5.33 ± 0.02 Dendrobium mutabile 2.56 ± 0.72 until 6.54 ± 0.73, Bulbophyllum blume 2.76 ± 0.09 until 6.07 ± 0.43 and Bulbophyllum beflorum 1.04 ± 0.07 sampai 1.35 ± 0.16. Karyotype patterns all orchids studied 2n = 38 m except Coelogyne spesiosa 2n = 37 m+ 1 ak and Dendrobium mutabile 2n = 37 m+ 1 sm.
PERBAIKAN GENETIK ANGGREK ALAM Vanda spp MELALUI PERSILANGAN INTERSPESIFIK DALAM MENDUKUNG PERKEMBANGAN ANGGREK DI INDONESIA Sri Hartati; Sumijati Sumijati; Pardono Pardono; Ongko Cahyono
Caraka Tani: Journal of Sustainable Agriculture Vol 29, No 1 (2014): March
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (100.742 KB) | DOI: 10.20961/carakatani.v29i1.13304

Abstract

Efforts to improve the genetic quality of orchids have constraints on seed propagation techniques crosses and from crosses. The study aims to: (1) obtain a cross technique that can produce seeds with high fertility rates, (2) to obtain a new hybrid character which has advantages in flower. The study was conducted at the Center for Plant Conservation Kebun Raya Bogor LIPI. Research was an interspecific crosses that ♀ Vanda celebica x ♂ Vanda tricolor, ♀ Vanda celebica x ♂ Vanda dearei, ♀ Vanda celebica x ♂ Vanda insignis. And crosses the reciprocal is ♀ Vanda tricolor x ♂ Vanda celebica, ♀ Vanda dearei x ♂Vanda celebica, ♀ Vanda insignis x ♂Vanda celebica. Crosses performed by crossing parent elected as male or female parent. Activities include characterization of elders, crosses, harvesting, seed dispersal, seed Deployment and transfer done aseptically in tissue culture laboratory. Observations included the successful crossing, when formed fruit, ripe fruit, and formed protokorm. Of research produced 5 series hybrid to form protokorm is Vanda dearei x Vanda Celebica, Vanda celebica x Vanda dearei, Vanda insignis x Vanda celebica, Vanda celebica x Vanda insignis, Vanda celebica x Vanda tricolo. The success of crosses 33%- 100%, time required for seed maturation varies between 122-262 days, 16-23 days old germinated.
Co-Authors Linayanti Darsana Pardono Pardono Sri Hartati Sumijati Sumijati