Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal Tropical Animal Science Journal
S. M. Z. Ariffin
Department of Veterinary Pre-Clinical Sciences, Faculty of Veterinary Medicine, Universiti Putra Malaysia
Agriculture, Biological Sciences & Forestry Energy

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Tropical Animal Science Journal

 1 
Prevalence of Methicillin Resistant Staphylococcus aureus in Raw Goat Milks from Selected Farms in Terengganu, Malaysia M. H. Chai; T. A. M. Faiq; S. M. Z. Ariffin; Z. Suhaili; M. Z. Sukiman; M. F. Ghazali
Tropical Animal Science Journal Vol. 43 No. 1 (2020): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (473.279 KB) | DOI: 10.5398/tasj.2020.43.1.64

Abstract

The emergence of antimicrobial drug resistant bacteria has been a concern worldwide. One of bacteria that has been reported to develop resistance is Methicillin Resistant Staphylococcus aureus (MRSA). Recent studies showed that Livestock Associated MRSA (LA-MRSA) was found in domestic food animals and their handlers. The aim of this study was to investigate the prevalence of MRSA in goat milk of goat farms located in Terengganu. A total of 664 udder milks were taken from 332 goats at 40 selected farms within Terengganu state. Then, screening of bacteria and isolation of suspected S. aureus isolates in the milk samples was done using selective agar, Gram staining and biochemical tests. The identity of the bacteria isolated was further confirmed using PCR where specific designed primers were used to detect the presence of nuc gene of S. aureus (278bp) and mecA gene (533bp) of MRSA. Both S. aureus and MRSA isolates were also tested for their susceptibilities toward the antimicrobial drugs. Fifty milk samples were found to contain S. aureus and one of the S. aureus isolates were MRSA. The bacteria isolates were found to have higher tendency to be resistance toward Penicillin (26.0%) and Oxacillin (12.0%). This study provides useful data on the current status of MRSA prevalence in small ruminant’s milk, which can be used to prevent transmission of LA-MRSA to human and other animals.
Molecular Detection and Antibiogram of ESBL-Producing and Carbapenem-Resistant Escherichia coli from Rabbit, Swine, and Poultry in Malaysia M. H. Chai; M. Z. Sukiman; N. Jasmy; N. A. Zulkifly; N. A. S. Mhd Yusof; N. M. Mohamad; S. M. Z. Ariffin; M. F. Ghazali
Tropical Animal Science Journal Vol. 45 No. 1 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.1.16

Abstract

The emergence of multidrug-resistance Enterobacteriaceae such as extended-spectrum β-lactamase (ESBL) producing Escherichia coli (E. coli) and carbapenem-resistant E. coli (CREC) has become an urgent veterinary and public health threat. These multidrug-resistant microorganisms are frequently associated with diseases that have high mortality with limited treatment options. This study aims to investigate the prevalence of ESBL producing E. coli and CREC from the rabbit, swine, and poultry and to determine the antibiogram profile of these E. coli isolates. In this study, 400 fecal swab samples were collected from rabbits, swine, and poultry from several selected animal farms in Malaysia. After incubation and isolation processes, suspected E. coli isolates were subjected to a PCR test to confirm the identity of the bacteria. The antibiogram of the E. coli isolates was determined via the Kirby Bauer disk diffusion method. A total of 212 (53%) E. coli isolates were isolated from rabbits (51 isolates), poultry (110 isolates), and swine (51 isolates). Screening of antimicrobial resistance genes revealed twelve ESBL producing E. coli (3%; 12/400). Two ESBL producing E. coli were also carrying carbapenemase gene (BlaNDM), indicating ESBL producing and carbapenem-resistant E. coli (ESBL-CREC) in poultry fecal swab samples. The bacteria isolates were found to show resistance against nine antibiotics, including ertapenem, ampicillin, and amoxicillin-clavulanate. A total of 3.3% (7/212) of the E. coli isolates were found to be multidrug-resistance. This study demonstrated the presence of ESBL-producing E. coli and ESBL-producing CREC from poultry fecal swabs in Malaysia.
Co-Authors M. F. Ghazali M. F. Ghazali M. H. Chai M. H. Chai M. Z. Sukiman M. Z. Sukiman N. A. S. Mhd Yusof N. A. Zulkifly N. Jasmy N. M. Mohamad T. A. M. Faiq Z. Suhaili