The research was aimed at investigating the effect of natural zeolite of Tasikmalayan deposit and urea fertilizer on ammonia volatilization, fertilizer usage efficiency, and rice yield. This research was a pot experiment using Eutrudept soil type conducted in a glass house. The experiment was factorially arranged in a randomized block design. The factors tested were level of natural zeolite (0, 1000, and 2000 kg/ha) and urea fertilizer (0, 100, and 200 kg N prilled urea/ha and 0, 100, and 200 kg N supergranule urea/ha). Ammonia emitted was determined using Sulfuric Acid Entrapment System. Application of natural zeolite reduced volatilized N by 46%, from 78.78 mg N/pot to 42.11 mg N/pot. The highest volatilized N was observed at the application of prilled urea at 200 kg N/ha (197.23 mg/pot).