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All Journal The Journal of Experimental Life Sciences (JELS) Indonesian Journal of Halal Research
Estri Laras Arumingtyas
University of Brawijaya
Religion Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience Other

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Design and Performance Test of Specific Primers to Detect Bovine DNA Fragments using Multiplex PCR Technique for Halal Authentification Joni Kusnadi; Kevin Hohn Hernandi; Khotibul Umam Al-Awwaly; Estri Laras Arumingtyas; Hilda Maghfirotu Hakiki; Nur Istianah
Indonesian Journal of Halal Research Vol 4, No 2 (2022): August
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ijhar.v4i2.15573

Abstract

Adulterating meat products with several species, including non-halal species, is often found in commercial products. This study aims to design and validate the Cytochrome c oxidase subunit I (CO1) primers to detect the non-halal species. A pair of species-specific primers encoding the CO1 gene were designed to amplify bovine DNA, tested for specificity, and applied in multiplex polymerase chain reaction (PCR) technique with D-loop primers for pigs, Cyt-b for rats, and 12S rRNA for dogs. The CO1 primers, along with D-loop primers for porcine, Cyt-b primers for rats, and 12S rRNA primers for dogs can be used to detect specific bovine DNA with a size of 279 bp and sequence similarity of 96%. In addition, dog, rat, and porcine DNA were amplified at 101, 603, and 951 bp, respectively. These four primers are specific and can amplify the target DNA to detect non-halal meat component contamination in a single reaction process.
Effect of Bulbils Position on Leaf Branches to Plant Growth Responses and Corms Quality of Amorphophallus muelleri Blume Retno Mastuti; Nunung Harijati; Estri Laras Arumingtyas; Wahyu Widoretno
The Journal of Experimental Life Science Vol. 8 No. 1 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1140.975 KB) | DOI: 10.21776/ub.jels.2018.008.01.01

Abstract

Bulbil (corm leaf) is one material source of vegetative propagation in Amorphophallus muelleri Blume. Based on the position in branches there are two types of bulbil, middle and edge bulbils, which are different in shape and size. It has been reported that bulbil size affected not only the growth response of seedlings and plants but also the quality of the produced corms. Therefore, the objectives of this experiment were to study the effect of bulbils origin on A. muelleri Blume (Porang) growth and the quality of harvested corms. The quality of corms was assessed based on glucomannan and (CaOx) content. Bulbils from the middle and edge of branches were grown in polybag (f 5 cm) containing compost:fertilizer (1:1) mixed media under 40% shade. Each bulbil origin was repeated 8 times. The results showed that growth responses of plant from middle bulbil were significantly better than that from edge bulbil. However, at the harvesting time, the weight and diameter of corms derived from both types of bulbils were not significantly different. The glucomannan and calcium oxalate (CaOx) content of harvested corms grown from middle bulbil tends to be higher than that from edge bulbil.Keywords: branching, calcium oxalate (CaOx), germination, glucomannan, Porang.
Co-Authors Hilda Maghfirotu Hakiki Joni Kusnadi Kevin Hohn Hernandi Khotibul Umam Al-Awwaly Nunung Harijati Nur Istianah Retno Mastuti Wahyu Widoretno