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Antibacterial of Clostridium botulinum From Eagle Flower (Clitoria ternatea L.) Kombucha Body Wash as a Pharmaceutical Biotechnology Product Hari Hariadi; Titin Sulastri; Firman Rezaldi; Susanti Erikania; Rina Nurmaulawati
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): January - March
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.4470

Abstract

Liquid bath soap made from telang flower kombucha fermented solution has potential as a pharmaceutical biotechnology product in inhibiting the growth of Clostridium botulinum bacteria. The aim of this study was to formulate and prepare a liquid bath soap with an active ingredient based on fermented eggplant kombucha solution in inhibiting the growth of Clostridium botulinum bacteria. The research design was carried out experimentally by making four liquid body wash preparations consisting of body wash base ingredients without active substances as negative controls. The basic ingredients for bath soap with the active ingredient are fermented butterfly pea flower kombucha solution with a concentration of 20%; 30%; and 40%. Disc diffusion is a test method used to inhibit the growth of test bacteria from each soap preparation. One way ANOVA and post hoc analysis are methods used to statistically analyze all liquid body wash preparations to see their effect on the growth of the test bacteria. The results of the study were proven and it can be concluded that based on one way ANOVA with a p value <0.05, 40% concentration of telaga kombucha bath soap is the best treatment for inhibiting the growth of test bacteria with an average diameter of the resulting inhibition zone of 18.50 mm and entering the strong category. The concentration of 40% was significantly different in inhibiting the growth of the test bacteria compared to the concentrations of 20% and 30% but not significantly different from the bath soap on the market as a positive control.
Study of Computational Biotechnology (Bioinformatics) on Telang Flower Kombucha (Clitoria Terantea L) as an Immunomodulator to Suppress Immunoglobulin E (IgE) for Allergy Sufferers Nurrizka Kurniawati; Ika Sutra Permirahayu Aji Saputri; Firman Rezaldi
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): April-June
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.4895

Abstract

Telang flower kombucha has been known to have pharmacological movement in vitro in repressing the development of microbes and organisms. Besides that, it also has the potential in vivo to inhibit cholesterol in livestock, so that it can be used or utilized as an active ingredient in drugs and cosmetics. In addition, allergy can cause immunity to tend to decrease due to the synthesis of immunoglobulin E protein, so this study aims to provide an initial picture for allergy sufferers through computational biotechnology or bioinformatics studies on the vitexin compound in tela flower kombucha to inhibit the formation of IgE as a cause of allergy based on its binding affinity value. The research method used was molecular docking and validation of the vitexin compound in butterfly pea flower kombucha in inhibiting IgE formation. The results of single point calculations and geometric optimization of the vitexin compound in butterfly pea flower kombucha were 2.99585 kcal/mol and 5.20442 kcal/mol respectively. The research results show that based on the binding affinity value of -7.1, vitexin contained in telang flower kombucha is able to inhibit the formation of Immunoglobulin E (IgE) as one of the causes of allergies.
Pharmaceutical Biotechnology Products in the Form of Hand Washing Soap Kombucha Bunga Telang (Clitoria ternatea L) as Antibacterial for Salmonella thypi and Listeria monocytogenes Devita Anugrah Anggraini; Firman Rezaldi; Anggita Sofianti; Irmawati Mathar; Yuliana Kolo
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): April-June
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.4892

Abstract

The growth of Salmonella thypi and Listeria monocytogenes bacteria can be inhibited by butterfly pea flower kombucha. This is because telang flower kombucha has pharmacological activity so it can be developed in the pharmaceutical field. This research needs to be carried out to make hand washing soap made from an active solution of telang kombucha diffusing solution so that it can stop the growth of Salmonella thypi and Listeria monocytogenes bacteria. The research method uses laboratory experiments. The data obtained were then analyzed using One Way Analysis of Variance and post hoc. The results of the study showed that the formulation and preparation of hand washing soap with a concentration of 40% active ingredient in a solution of butterfly pea flower was able to stop the growth of both bacteria with a P value <0.05. In conclusion, these two bacteria can be inhibited with hand washing soap containing 40% of a decoction of butterfly pea flower kombucha.
Effect of Biotechnological Fermentation Waste Kombucha Flower Telang (Clitoria ternatea L) as Liquid Fertilizer on The Growth of Sawey (Brassica chinensis var. parachinensis) Hari Hariadi; Firman Rezaldi; Fajar Hidayanto; Ade Sumiardi; Mu'jijah Mu'jijah; Muhammad Faizal Fathurrohim; Yuliana Kolo; Syariful Mubarok
Jurnal Biologi Tropis Vol. 23 No. 3 (2023): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i3.5081

Abstract

Liquid organic fertilizer comes from nature and has the potential to improve biological, physical and soil properties because it contains nutrients that have the potential to increase plant growth. This research was conducted to determine liquid fertilizer made from telang kombucha fermentation waste on the growth of mustard plants. The method used was a completely randomized design with a single factor, namely the difference in the concentration of liquid organic fertilizer given 6 treatments with 5 replications for each treatment. The research data were analyzed statistically through ANOVA at a significance level of 95%. Parameters in this study were plant height, number of leaves, fresh weight and dry weight. The results of this study prove that the spraying treatment of liquid organic fertilizer containing kombucha fermentation waste of butterfly pea flowers has an effect on the growth parameters of mustard plants. The conclusion is that liquid organic fertilizer from the waste of fermented kombucha flower butterfly pea at a concentration of 5 mL/L can increase plant height, number of leaves, wet and dry weight of mustard plants.
Growth Inhibition Test of Gram and Negative Bacteria in Pharmaceutical Biotechnology Products in the Form of Hand Sanitizer Formulations Based Fermented Telang Flower Kombucha Hari Hariadi; Muhammad Andry; Muhammad Amin Nasution; Ade Sumiardi; Firman Rezaldi; Suseno Amien; Yusep Ikrawan
Jurnal Biologi Tropis Vol. 23 No. 3 (2023): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i3.5219

Abstract

Telang flower kombucha is proven to have various pharmacological activities, so that it can be developed as medicines, cosmetics, and its waste can be applied in agriculture as an ingredient in organic liquid fertilizer to support plant development and growth. This research aims to make hand sanitizer gel formulations and preparations with active butterfly pea flowers. Disk diffusion is used to stop gram-positive and gram-negative bacteria from growing. Post hoc analysis and one-way ANOVA were used to analyze the data. The one-way ANOVA analysis yielded results with a significance level of P<0.05, allowing the post hoc test to continue. When tested at a sugar concentration of 40%, the butterfly pea flower kombucha hand sanitizer significantly outperformed the control when it came to inhibiting the growth of the test bacteria. The best way to stop the test bacteria from growing is to use a concentration of 40%.