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Indirani M.S Sembiring, Indirani M.S
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Journal : Proceeding International Conference on Global Resource Conservation

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Assessing genetic diversity of Indonesian Andaliman (Zanthoxylum acanthopodium DC.) germplasm based on 7 RAPD markers Putri, Lollie A.P; Sinaga, Ayu O.G; Sembiring, Indirani M.S; Sinaga, Ann
Proceeding International Conference on Global Resource Conservation Vol 6, No 1: Proceeding of 6th ICGRC 2015
Publisher : Proceeding International Conference on Global Resource Conservation

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Abstract

This is the first publication on Zanthoxylum acanthopodium of  Indonesian germplasm based on RAPD markers. We used 7 polymorphic RAPD markers in Z. acanthopodium DC to characterize 30 accessions from three district of  North Sumatera, Indonesia. Our principal objectives are to study the genetic diversity of  the genitors. The RAPD analyse was used with 7 random primers: OPH-06, OPC-12, OPN-03, OPN-09, OPD-13, OPI-20 and OPM-01. These seven primers showed 55 bands. Further genetic diversity coefficient and the phylogenetic dendogram were developed using the Darwin 5.05 software. The Principal Coordinates Analysis (PCoA) identifies, with axes 1 and 2 explaining 39.96 % of the total molecular variation. From the analysis of genetic distance and dendogram, 30 accessions of North Sumatra’s andaliman grouped into 3 clusters. This study showed high genetic dissimilarity inter origin and intra origin.Keywords:  Genetic diversity; North Sumatera; RAPD marker; Zanthoxylum acanthopodium
Co-Authors Ann Sinaga, Ann Ayu O.G Sinaga, Ayu O.G Lollie A.P Putri, Lollie A.P