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Agung Wahyu Susilo
Pusat Penelitian Kopi dan Kakao Indonesia
Agriculture, Biological Sciences & Forestry

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Journal : Pelita Perkebunan

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Development of Selection Criteria on Bean Weight Character of Cocoa (Theobroma cacaoL.) through Path Analysis Approach Indah Anita Sari; Agung Wahyu Susilo
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 29 No 3 (2013)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v29i3.10

Abstract

Path coefficient analysis is frequently used for development of selection criteria on various type of plants. Path analysis on this research was conducted to find the selection criteria of yield component which directly affect bean weight. In addition to the value of path analysis coefficient, genetic variation coefficient, heritability and the value of genetic progress were also studied. The study was conducted at the Indonesian Coffee and Cocoa Research Institute. The research used randomized complete block design consisting of 14 accession numbers and each consisting of three replications. Pod girth, pod length, pod weight, wet beans weight per pod, number of normal beans per pod, number of abnormal beans per pod, dry weight per normal bean, and shell content were observed. The results showed that the pod weight character had an important role in determining the dry weight of normal bean. The character had a positive genotype correlation coefficient values which was high and significantly different (r=0.46) for dry weight per normal bean, considerable direct influence (P=0.479), moderate of the genotype variation coefficient (9.6%), and high genetic progress (95.23). Character of wet bean weight per pod could also be used indirectly for the selection criteria for dry weight per normal bean based on genetic variation coefficient value (11.88%), genetic progress value (82.48), and direct effect on dry weight per normal bean had positive value (P=0.006). Key words: Selection criteria, dry weight per bean, path analysis,Theobroma cacaoL.
Selection and Estimation the Genetic Parameters of Rootstock Characteristics on Cocoa Seedling of Half-sibs Families Agung Wahyu Susilo; Dyah Sulastri; Soeboer Djatiwaloejo
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 21 No 3 (2005)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v21i3.25

Abstract

For cocoa clonal propagation it need rootstock with good characteristics. Half sibs families is the most appropriate alternative for rootstock selection. This study was aimed to select and estimate genetic parameters of cocoa seedling on the half sibs families of DR 1, DR 2, ICS 60, DRC 15, BLC 4, DRC 16, KEE 2, ICS 13, KW 162, KW 163, PA 300, RCC 70, TSH 858, Sca 6 and Sca 12. Research was carried out in Kaliwining Experimental Station of Indonesian Coffee and Cocoa Research Institute. Genetic parameter were estimated based on variance and covariance component of the variables of the number of leaf, stem height, stem girth, leaf acreage, the volume of fresh root, the length of secondary root and the length of primary root which assessed along seed growth till 90 day after planting. The result showed that most of the recorded variables perform high category of narrow sense heritability (h 2>0.5). Stem girth, leaf acreage, volume of fresh root, length of secondary root and the length of primary root were assessed as the broad category of genetic variability (d2 A >2Sed 2 A ). Therefore, length of primary root and length of secondary root would be the main criterion for rootstock selection as their performance on broad category of genetic variability and high category of narrow sense heritability. Length of secondary root can be estimated based on the performance of stem girth (r = 0.5 * ) and leaf acreage (r = 0.23*) and the primary root can be stimated based on the performance of stem height (r = 0.81 * ) and leaf acreage (r = 0.72*). Based on positive selection of primary root lenght and negative selection of secondary root lenght it was identified that the families of KEE 2 and Sca 12 potentially would be good rootstock due to their long category of the primary root and short category of the secondary root that having characteristics of high water uptake and low vigor. Key words: selection, genetic parameter, half-sib familiy, rootstock, cocoa.
Relationship Between Pod Characteristics of Some Cocoa Clones (T. cacaoL.) and Their Resistance Response to Cocoa Pod Borer Agung Wahyu Susilo; Woerjono Mangoendidjojo; Witjaksono .
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 23 No 3 (2007)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v23i3.38

Abstract

Characteristic study on CPB resistance had been carried out by characterizing mesocarp layer of cocoa pod where egg laying and larva penetration are carried out. For this study, 4 clones were selected which performed different response to CPB, namely resistant clones of KW 514 and ARDACIAR 10, moderately resistant clone of KW 411 and the susceptible clone of RCC 72. Pod samples were classified as young and mature were detached from cocoa collection at the experimental station of Kaliwining, Indonesian Coffee and Cocoa Research Institute in Jember. Characterization was conducted based on microchemical method at the Microtechnique Laboratory of Gadjah Mada University to identify trichome appearance at upper layer of pod, distributed granules of tannin through mesocarp and lignification of the sclerotic. It was characterized that the trichome density performing resistant in which the resistant and moderately resistant clones had more number of trichome density than of the susceptible one. The distributed granules of tannin at young pod showed significant difference among those clones that indicate resistant characteristics. The number of distributed granules of tannin was higher of those the resistant and moderatly resistant clones than of the susceptible clones that were formed both at furrow and at ridge of pod. However, the granules of tannin were distributed more at ridge than at furrow where furrow were preferred by CPB for egg laying. The characteristic differences between the resistant and moderately resistant clones were identified based on the intensity of lignification at sclerotic tissue. The lignified tissue of the resistant clones perform more intensive and more compact than of the moderately resistant clones. Tissue of young pod of the moderately resistant and susceptible clones was not yet lignified. Thickness of lignified tissue at the furrow of resistant clone was higher than of the moderately resistant and susceptible clones. Key words : Theobroma cocoa L.,cocoa pod borer, resistant, mesocarp, sclerotic, trichome, tannin.
Self-Compatibility Crosses of Several Cocoa Clones Agung Wahyu Susilo
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 22 No 3 (2006)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v22i3.47

Abstract

Self compatibility cross in cocoa is a useful criterion in utilizing germplasm collection. Evaluation of self-compatibility crosses on cocoa clones has been carried out in Kaliwining Experimental Station of ICCRI by treating artificial self-pollination. The observed clones were TSH 858, ICS 60, ICS 13, UIT 1, KW 162, KW 165, KW 163, DR 1, DR 2, DRC 16, DRC 15, KKM 22, Na 32, Na 33 and DR 38. Self-compatibility crosses were identified by percentage of fruit set evaluated during 6 consecutive weeks after pollination. The results showed that the percentage of fruit set were significantly different among clones in the range of 0—46.34%. Three groups of compatibility of cocoa clones were identified i.e. first, self-incompatible clones of DR 1, Na 32, Na 33; second, partially self compatible clones of DR 38, TSH 858, ICS 60, ICS 13, UIT 1, KW 162, KW 165, KKM 22; and third, self-compatible clones of DR 2, DRC 16,DRC 15, KW 163. Clones which perform self-incompatible and partially self-compatible furthermore can be used as female parents in the production of hybrids. Key words: self-compatibility, Theobroma cacao, clones.
Co-Authors Dyah Sulastri Indah Anita Sari Soeboer Djatiwaloejo Witjaksono . Woerjono Mangoendidjojo