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All Journal Buletin Veteriner Udayana
Wayan Bebas
Laboratorium Reproduksi Veteriner Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Penambahan Vitamin E pada Pengencer SemenLife® Terhadap Kualitas dan Lama Simpan Spermatozoa Babi Landrace Gede Putra Sanjaya; Wayan Bebas; Tjok Gde Oka Pemayun; I Gusti Ngurah Bagus Trilaksana; Desak Nyoman Dewi Indira Laksmi; Ni Nyoman Werdi Susari
Buletin Veteriner Udayana Vol. 15 No. 6 December 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i06.p16

Abstract

Artificial insemination in pigs in Indonesia still uses liquid semen. Therefore, specific media are needed to maintain the quality of pig spermatozoa. This study aims to determine the effect of adding vitamin E (a-tocopherol) to Semenlife® (INTTEC) diluent on the quality and shelf life of spermatozoa of landrace pigs. The sample used was fresh semen which was collected using a mass technique taken from the UPTD. Regional Artificial Insemination Center, Livestock Breeding and Forage/Livestock Institute of Bali Province. A total of 24 samples consisting of 12 samples in the SemenLife® (INTTEC) diluent and 12 samples in the SemenLife® (INTTEC) diluent + vitamin E 400 µg/ml were stored at 15-18oC. Observation of spermatozoa quality (viability and motility) and storage time was carried out for 120 hours with observations of spermatozoa quality under a microscope every 24 hours, then DNA fragmentation was tested using the Pocrine 8-Hydroxy-desoxyguanosine ELISA Kit. The graph results of Duncan's test showed that the addition of 400 µg/ml vitamin E had a significant effect on the quality of spermatozoa, especially in terms of motility which was maintained at 48 hours and the lowest DNA fragmentation occurred at 72 hours. Furthermore, the addition of vitamin E was not able to extend the shelf life of landrace pig spermatozoa in diluent. Where, there was a decrease in motility and an increase in DNA fragmentation, respectively at 72 and 96 hours, in viability the decrease occurred periodically from the 0 hour to 120 hours. From the results of the quality and shelf-life tests obtained, spermatozoa can then be tested by conducting a fertility test to find out whether the results obtained can increase litter size.
Pengaruh Lama Simpan Semen Ayam Ekor Panjang dengan Pengencer Kuning Telur dan Air Kelapa Wulung pada Suhu 5oC Dyah Risma Aprilia Enji; Wayan Bebas; I Gusti Ngurah Bagus Trilaksana
Buletin Veteriner Udayana Vol. 15 No. 6 December 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i06.p19

Abstract

Long-tailed chickens or commonly called onagadori chickens are classified as rare and endangered animals. This ornamental chicken has the advantage of its long tail reaching 10 meters. This study aims to determine the effect of storing semen with egg yolk coconut water diluent stored at cold temperatures on motility, abnormalities and viability. The design in this study used a completely randomized design (CRD) with 7 treatment groups for storage duration, namely 0 hours, 12 hours, 24 hours, 26 hours, 48 hours, 60 hours, 72 hours. The research object used was male long-tailed chickens that were sexually mature at the age of 6-8 months. Chicken semen storage technique by massaging the chicken's back (massage). Semen examination was carried out macroscopically and microscopically. Viability and abnormality of spermatozoa were observed using the eosin negrosin staining method. This study concluded that the motility, viability and spermatozoa abnormalities of long-tailed chickens in egg yolk coconut water diluent could be maintained for 48 hours at 5°C storage temperature with 52.41% motility, 50.15% viability and 7.33% abnormality. In order to obtain optimal results in supporting the success of artificial insemination, long-tailed chicken semen with egg yolk diluent and coconut water should not be stored for more than 60 hours.
Co-Authors Desak Nyoman Dewi Indira Laksmi Dyah Risma Aprilia Enji Gede Putra Sanjaya I Gusti Ngurah Bagus Trilaksana Ni Nyoman Werdi Susari Tjok Gde Oka Pemayun