Free-range chicken is one of the livestock favored by the people of Indonesia both eggs and meat. When breeding, of course, breeders will be faced with obstacles or problems, one of which is diseases caused by blood parasites, for example Leucocytozoonosis. Leucocytozoonosis or malaria-like disease is a protozoan disease that attacks the blood of poultry, caused by the parasite Leucocytozoon spp Given the level of losses caused by infection from these parasites, this study needs to be done in order to determine the prevalence of Leucocytozoon spp infection in free-range chickens in Bali. This study used blood smears from 100 chickens in Bali as samples. The method used in this study was in the form of making thin blood review preparations with 10% Giemsa staining and microscopic examination. The parameter measured was the presence or absence of Leucocytozoon spp in thin blood review preparations. The data obtained were analyzed using descriptive analysis and to determine the relationship between sex and region of origin of chickens with the prevalence of infection Leucocytozoon spp was analyzed using chi-Square analysis. The results showed that the prevalence of Leucocytozoon spp infection in free-range chickens in Bali was 30%. After analysis using the chisquare test, it was stated that sex was not related (P>0.05), while the location of the sampling area was related (P<0.05) with the prevalence of Leucocytozoon spp infection in free-range chickens in Bali. It can be concluded that the prevalence of Leucocytozoon spp infection in free-range chickens in Bali is 30%, with the sampling area associated with the prevalence of Leucocytozoon spp infection in free-range chickens in Bali, while sex is not related. We recommend that farmers improve maintenance management given the high prevalence of infection.