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All Journal Jurnal Farmasi Galenika
Veny Larasti
Program Studi pendidikan dokter, FakultasKedokteran, Universitas Sriwijaya
Medicine & Pharmacology Public Health

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Isolasi Dan Karakterisasi Bromelain Bonggol Nanas Dari Limbah Industri (Ananas comosus L. Merr.) Laida Neti Mulyani; Veny Larasti; Dwi Handayani; Herlina Herlina
JURNAL FARMASI GALENIKA Vol 11 No 1 (2024): Jurnal Farmasi Galenika Vol 11 No 1
Publisher : Universitas Bhakti Kencana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70410/jfg.v11i1.328

Abstract

Waste of pineapple stem (Ananas comusus L. Merr.) has not been optimally utilized, although it is known that pineapple contain bromelain enzym. This research aimed to isolation and characterization bromelain from pineapple stem. Enzim was extracted from pineapple stem to produce crude enzim of bromelain. Isolation bromelain was done by amonium sulphate precepititation and continued by dialysis. Characterization to determined molecular weight, protein content and protease activity. The result of fractionation by amonium precipitation showed the highest specific activity on amonium sulphate 40-60% fraction 1011,98 U/mg. Caharacterization of purified bromelain showed that molecular weight of bromelain is 23,69 dan 24,72 kD with proteint content 2,78 mg/mL and protease activity is 2833,33 U/mL. This study concluded that the pineapple stem has bromelain enzym with the protease activity.
Co-Authors Dwi Handayani Herlina Herlina Laida Neti Mulyani Laida Neti Mulyani