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Datt, Thakur
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Journal : Medical and Health Science Journal

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Transcriptional analysis of Metalloprotease, Metallocarboxypeptidase, Isocitrate lyase, Citrate synthase, Malate synthase and Dipeptidylpeptidase V of Trichophyton rubrum isolates from dermatophytosis patients. Datt, Shyama; Datt, Thakur; Das, Shukla; Battacharaya, Sambit Nath
Jurnal Ilmu Kesehatan dan Kesehatan Vol 7 No 02 (2023): AUGUST
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mhsj.v7i02.4044

Abstract

Introduction: Dermatophytes are pathogenic fungi that cause cutaneousinfection of human and animal and grow exclusively on the stratumcorneum, nail and hair. Methods: The present study was conducted on 160 samples from clinicallydiagnosed onycomycosis patients, further subjected to culture from nailsamples of patients attending dermatology OPD of a tertiary care hospital,Delhi from January 2016 to December 2018 Results: In a soy protein culture, a substantial proteolytic activity was seen, which was secreted by Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis. This proteolytic activity was 55–75% inhibited by O-phenanthroline, which authenticate that metalloproteases were secreted by all three species. A consensus probe was constructed on previously characterized genes, which encodes metalloproteases (MEP) of the M36 fungalysin family in Aspergillus fumigatus, Aspergillus oryzae and M. canis. From genomic libraries of T. rubrum, T. mentagrophytes and M. canis, T. rubrum & T. Mentagrophytesa five-member MEP family was isolated and also secretes MCP A & MCPB, MEP 3, MEP4, IL, CS, MS AND DPP V of the M14 family accordingto the MEROPS proteolytic enzyme database. Conclusion: This study shows that Metalloprotease, Metallocarboxypeptidase, Isocitrate Lyase, Citrate Synthase, Malate Synthase and Dipeptidylpeptidase V are expressed in Trichophyton Rubrum Isolates from Dermatophytosis Patients.
In-vitro Susceptibility Testing of Dermatophytes Isolated in Delhi (India) Against Five Antifungal Drugs Datt, Shyama; Datt, Thakur; Singh, N.P
Jurnal Ilmu Kesehatan dan Kesehatan Vol 8 No 02 (2024): AUGUST
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mhsj.v8i02.5727

Abstract

Background: Over the past few decades, cases of dermatophytosis have been on the rise. Recently, the introduction of newer, less toxic antifungal drugs has improved treatment options. However, the prolonged use of antifungals has led to the emergence of acquired resistance among strains that were previously susceptible, as well as an increase in infections caused by less common species. This scenario underscores the growing need for rapid and accurate antifungal susceptibility testing methods. In this study, antifungal susceptibility was assessed using the in-vitro micro broth dilution method, adhering to the CLSI M38-A guidelines Methods: 60 clinical specimens were collected from Nail and skin of the patients of dermatophytosis from Delhi (India). Minimal inhibitory concentration (MIC) was performed in microtiter plates with U-bottom and incubated at 35° C. Reading were taken after 48 & 96 hrs of incubation for Trichophyton mentagrophytes and Trichophyton rubrum, against 5 antifungal drugs namely fluconazole, itraconazole (triazoles), griseofulvin, terbinafine and Luliconazole. Results: Most of the dermatophytes had uniform patterns of susceptibility to the antifungal agents tested. Low MIC values as 0.03μg/mL were found for 33.3%, 31.6% and 15% of isolates for itraconazole and terbinafine, respectively. Conclusion: In conclusion, it may be useful to undertake periodical screening programs to detect the antifungal susceptibility of newer antifungal agents.
Genotypic and Phenotypic Identification of Dermatophytes among Clinical Isolates Datt, Shyama; Datt, Thakur; Singh, Narender Pal
Jurnal Ilmu Kesehatan dan Kesehatan Vol 9 No 1 (2025): FEBRUARY : Fragmentation In Modern Healthcare Approaches Amid Rising Chronic And
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mhsj.v9i1.6653

Abstract

Background: The prevalence of superficial fungal infections varies basedon environmental and hygienic conditions. Dermatophytes, particularlyTrichophyton rubrum and T. mentagrophytes, are keratinophilic fungiresponsible for the majority of these infections. Their ability to produceproteases and keratinases active at acidic pH facilitates colonization ofhuman skin. This is a fundamental mechanism observed in Trichophytonrubrum and Trichophyton mentagrophytes in establishing superficialinfection.Methods: A total of 200 clinical samples from patients with suspecteddermatophytosis were examined using potassium hydroxide (KOH) mountand culture techniques. Culture-positive isolates were further identifiedthrough conventional mycology and molecular methods, including PCR andsequencing using species-specific ITS2 primers for T. rubrum and T.mentagrophytes. Clinical profiles, treatment history, and recurrence patternswere also analyzed.Results: Among 200 KOH-positive samples, 140 yielded culture-positiveresults, of which 60 isolates were identified as dermatophytes. Molecularidentification confirmed 20 isolates as T. rubrum and 40 as T.mentagrophytes. Clinically, 43.34% of cases were naïve infections, 50%had ongoing antifungal treatment for 1–2 years (recalcitrant cases), and6.66% were categorized as reinfections due to recurrence of lesions posttherapy. The mean patient age was 30.02 ± 10.95 years, with a male-tofemale ratio of 2:1.Conclusion: Trichophyton mentagrophytes was more prevalent than T.rubrum among dermatophyte-positive cases. The high rate of recalcitrantand recurrent infections highlights the need for accurate species-leveldiagnosis using molecular tools to guide effective antifungal managementstrategies.
Co-Authors Battacharaya, Sambit Nath Das, Shukla Datt, Shyama Singh, N.P Singh, Narender Pal