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All Journal Vegetalika Jurnal Biologi Universitas Andalas
Zozy Aneloi Noli
Departemen Biologi, FMIPA, Universitas Andalas
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Pengaruh Benzil Aminopurin (BAP) terhadap Induksi Tunas dari Nodus anggrek Dendrobium mussauense Media Media; Zozy Aneloi Noli
Jurnal Biologi Universitas Andalas Vol 12, No 1 (2024)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.12.1.35-41.2024

Abstract

Dendrobium mussauense has attractive flowers and potential as a parent of a hybrid orchid. The color of this species is cream with violaceous purple venations on the labellum. D. mussauense is an orchid species listed as vulnerable in IUCN. This species is not located in protected areas nor known to be conserved ex-situ. Clonal propagation is an important biotechnological tool for the conservation of orchids. Thin cell layer (TCL) is a micropropagation method using thin-sized explants that facilitate the diffusion of nutrients to tissues. This study examined induction shoot through two explant cutting techniques, namely TCL and non-TCL explant, at various concentrations of BAP. The non-TCL explant was more effective than the TCL explant in inducing the shoot of D. mussauense. The half-strength Murashige and Skoog (MS) medium supplemented with 0.9 mg.L-1 BAP was a suitable concentration to induce the shoot of D. mussauense through non-TCL explant. The average shoot length was 1,8 cm, and the average number of shoots was 5/explant.
Respon Eksplan Tunas Homalomena rubescens terhadap Kualitas Cahaya dan Efeknya pada Pengayaan Metabolit Sekunder Zikra Lareta Putri; Zozy Aneloi Noli; M Idris
Vegetalika Vol 15, No 2 (2026): In Publish
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.109720

Abstract

Homalomena rubescens (Araceae) is an ornamental plant known for its distinctive aroma from the roots, rhizomes, and leaves. Species of the genus Homalomena are rich in secondary metabolites with potential pharmaceutical applications, including anti-Alzheimer, anti-inflammatory, antibacterial, and antioxidant properties. Enrichment of secondary metabolites in plants can be achieved through environmental modifications, e.g. light quality. In vitro techniques, particularly the use of light-emitting diodes (LEDs), are valuable for studying the effects of light quality. This study aimed to evaluate the effects of monochromatic light (blue; 400-500 nm and red; 600-700 nm) on the growth and secondary metabolite enrichment in H. rubescens shoots. Additionally, the response of two Homalomena species (H. rubescens and H. pendula) to callus induction under different light treatments was assessed. Polychromatic light (white; 400-700 nm) served as positive control, while total darkness acted as the negative control. Light treatments were applied during a 12-hour photoperiod. Results showed that red light enhanced shoot initiation, leading to a higher number of shoots, while blue light promoted increased shoot height and resulted in darker green shoots. Phytochemical analysis indicated that blue light enhanced the accumulation of flavonoids, phenols, and terpenoids. Additionally, blue light improved callus induction in both species. These findings suggest that red light is more effective in promoting shoot growth, while blue light supports secondary metabolite enrichment.
Co-Authors M Idris Media Media Zikra Lareta Putri