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All Journal ANNALES BOGORIENSES
Prasetyoputri, Anggia
Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology

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Journal : ANNALES BOGORIENSES

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Enhancing the Immunogenicity of Subunit Vaccines by Utilisation of Particulate Vaccine Delivery Systems Prasetyoputri, Anggia; Kusharyoto, Wien
ANNALES BOGORIENSES Vol 17, No 2 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (543.649 KB) | DOI: 10.1234/76

Abstract

Control and eradication of a number of infectious diseases are primarily attributed to effective vaccination programs. A concerted effort is still imperative to develop novel vaccines and improve the immunogenicity of existing ones with regards to efficacy, immunogenicity and safety. Rational design of vaccines using subunit vaccines is a potentially safer alternative to conventional vaccines, yet they are poorly immunogenic without additional adjuvant. Using antigen carriers to enhance their immunogenicity in the forms of adsorption or encapsulation with a delivery system has been widely investigated as an alternative to currently available adjuvants. This review aims to elaborate on the existing nanotechnology being used to develop more immunogenic subunit vaccines, with focus on particulate delivery systems for development of prophylactic vaccine candidates.
The Development of A Bioassay Based on Heterologous Expression of M2 Ion-Channel Protein Prasetyoputri, Anggia; Yuliaty, Neti; Tuharea, Warda; Febyanti, Alisin Febyanti; Sunarko, Bambang; Atmosukarto, Ines I.C
ANNALES BOGORIENSES Vol 14, No 2 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (429.809 KB) | DOI: 10.1234/57

Abstract

Emerging resistant viral  strains combined with the  limited availability of antivirals  in  a pandemic scenario highlight the need for the development of novel influenza antivirals. A bioassay based on the M2 protein of influenza virus  -  a potential target for antivirals - was developed to screen endophytic microbial extracts. M2 can be synthesized using PCR, thus eliminating the need for the handling of infectious specimen. Following cloning of the M2 gene into a pET backbone, the resultant plasmid was transformed into BL21 (DE3) pLyss E. coli  cells.  Cultures of these cells were set up at 37C following inoculation with a starter culture, to reach an OD  at  600nm  (OD) of  0.4-0.6.  Once  at  the  required  OD,  the  culture  was  split  in  two  aliquots  and expression  of  the  M2  protein  was  induced  in  one  of  the  duplicates  with  the  addition  of  isopropyl  β-Dthiogalactopyranoside (IPTG). Bacterial growth was monitored at 60 -minute intervals. Exogenous expression of  the  M2  protein  has  been  reported to  decrease  host  cells  viability,  resulting  in  lower  OD 600 values. Our results  suggest  that  the  M2  protein  was  expressed  and  that  overexpression  of  this  protein  resulted  in consistently lower OD 600  values of induced cultures compared with that of uninduced cultures. Based on this principle,  extracts  can  be  screened  for  their  ability  to  block  M2  function  as  identified  by  increased  OD 600 values.   Keywords: influenza, M2, bioassay, gene synthesis, antiviral
Co-Authors Atmosukarto, Ines I.C BAMBANG SUNARKO Febyanti, Alisin Febyanti Kusharyoto, Wien Tuharea, Warda Yuliaty, Neti