Menarche is the first sign of puberty in adolescent girls, marking the maturity of reproductive function. Lack of knowledge about menarche can cause anxiety, especially among students with disabilities. Audio-visual media are considered effective because they can convey information in a more interesting and easily understood way for children with special needs (Pratiwi et al., 2022). The purpose of this study was to determine the effect of audio-visual media on knowledge about menarche among female students with disabilities in Bengkulu City in 2025. The research method used was a pre-experimental design with a one-group pretest-posttest approach. The sample consisted of 30 female students with disabilities who had not yet experienced menarche, selected using probability sampling techniques. Data collection was conducted using a questionnaire that had been tested for validity and reliability. Data analysis was performed using the Wilcoxon test. The results showed that before being exposed to audio-visual media, the majority of respondents were in the low knowledge category, with 19 individuals (63.3%), 9 individuals (30%) in the adequate category, and 2 individuals (6.7%) in the good category. After being provided with audio-visual media, almost all respondents were in the good category, namely 28 (93.3%) and 2 (6.7%) in the adequate category. The Wilcoxon test results showed a p-value of 0.001 (p 0.05), indicating a significant effect of audio-visual media on improving knowledge about menarche among disabled female students. Conclusion: Audio-visual media significantly influences the improvement of knowledge about menarche among disabled female students. This medium can serve as an effective educational alternative in reproductive health education.