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Nurnida, Rossy
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Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology

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Journal : Journal of Medical Laboratory Research

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UJI VARIASI KOSENTRASI DAYA HAMBAT METODE SUMURAN DAUN BINAHONG (Anredera cordifolia (Tenore) Steenis) PADA PERTUMBUHAN Candida albicans Fadillah, Muhammad Arief; Amrita Aulia Sugiarto; Hamtini, Hamtini; Nurnida, Rossy
Journal of Medical Laboratory Research Vol. 3 No. 1 (2024): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

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Abstract

Infections caused by Candida albicans are common health problems, especially among individuals with weakened immune systems. This study aims to evaluate the antifungal effectiveness of binahong leaf (Anredera cordifolia) decoction extract against the growth of Candida albicans using the well diffusion method. The extraction process was carried out by boiling the leaves using water as the solvent. The tested concentrations of the extract were 5%, 25%, 50%, 75%, and 100%. The tests were conducted using the well diffusion method on media inoculated with Candida albicans. The results showed that no inhibition zones formed at any tested concentrations of the binahong leaf extract (0 mm), indicating no antifungal activity against Candida albicans. Meanwhile, the positive control (ketoconazole) showed an average inhibition zone diameter of 60.25 mm. The inability to form inhibition zones is likely due to the degradation of active compounds during heating, the limitation of water in extracting non-polar compounds, and the poor diffusion of active compounds in the agar medium. Based on these findings, it can be concluded that binahong leaf extract obtained through boiling does not exhibit antifungal activity against Candida albicans using the well diffusion method.
Identification Of Candida albicans in Oral Mucosal Swabs Of Smokers In The Gang Siswa Raya Kampung Sirnagalih Kota Tangerang Fadillah, Muhammad Arief; Ratu Muawwadah; Ridwanulloh, Mohammad; Hamtini, Hamtini; Nurnida, Rossy
Journal of Medical Laboratory Research Vol. 3 No. 1 (2024): Journal of Medical Laboratory Research
Publisher : Politeknik Kesehatan Kementerian Kesehatan Banten

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Abstract

Candidiasis is a primary or secondary mycotic infection caused by members of the genus Candida and other genera, where Candida is one of the various forms of yeast. This study aims to determine the presence of Candida albicans fungus in oral mucosa swabs of smokers in the Gang Siswa Raya, Kampung Sirnagalih, Kota Tangerang based on smoking frequency and duration of smoking. This study uses a descriptive method, namely research with the aim of describing the presence of Candida albicans in oral mucosa swabs of smokers in the Gang Siswa Raya, Kampung Sirnagalih, Kota Tangerang using Sabouraud Dextrose Agar media and Germ tube tests. A total of 20 samples from 97 smokers data were collected. The results of the examination showed that there were fungi in the oral mucosa swab samples of smokers in the Gang Siswa Raya, Kampung Sirnagalih, Kota Tangerang as much as 40%. Then there is the growth of Candida albicans fungus in smokers with a smoking frequency of >3 years as much as 40%. Then there is the growth of Candida albicans fungus in smokers with a smoking frequency of >10 cigarettes a day as much as 35%.
Co-Authors Amrita Aulia Sugiarto Fadillah, Muhammad Arief Ratu Muawwadah Ridwanulloh, Mohammad