Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Vol 11, No 1 (2006): February 2006

Karakterisasi Protease Ekstraseluler Clostridium spp. T11-3

Natalia, Loli (Unknown)
Nathalia, Lily (Unknown)
Meryandini, Anja (Unknown)

Article Info

Publish Date
03 Nov 2019

Abstract

Protease is one of the leather commercial enzymes which is widely used such in food processing, medicine and leather industry. Clostridium sp T11-3 was isolated from Tiu Jeruk River in Nusa Tenggara Barat. Sequence analysis of 16S rRNA indicated that Clostridium spp T11-3 was closely related to C. bifermentans. This isolate produced maximum protease activity after 18 hours of cultivation in liquid media. Protease of Clostridium spp 11-3 displayed maximum activity at pH 5 and 60oC with casein as substrate. In the presence of 1 mM divalent ion Mg2+ the enzym activity increased to 141 %, while others ion divalent (Ca2+, Zn2+, Cu2+, Fe2+, and Co2+) inhibited protease activity.

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Journal Info
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Website

Abbrev

biota

Publisher

Universitas Atma Jaya Yogyakarta

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN ...