Research Journal of Life Science
Vol 6, No 2 (2019)

Isolation, and Identification of Diesel Oil Degrading Bacteria in Water Contamination Site and Preliminary analysis with Potential Bacterial Gordonia terrae

Ainun Ramadhani Tri Wahyuni (Fisheries and Marine Science Faculty, Brawijaya University)
Endang Yuli Herawati (Fisheries and Marine Science Faculty, Brawijaya University)
Andi Kurniawan (Fisheries and Marine Science Faculty, Brawijaya University)
Abd. Aziz Amin (Coastal and Marine Research Center, University of Brawijaya, Indonesia)



Article Info

Publish Date
03 Aug 2019

Abstract

Bioremediation is one of kind method which use of bacteria to degrade of hydrocarbon to be another compound. This study aims to get isolation of indigenous bacteria and potential test with Gordonia terrae on diesel oil bioremediation. The water sample was taken from Tanjung Perak Port of Surabaya, Indonesia where contaminated with diesel oil. Analysis of in-situ water quality parameters were consist of Dissolved oxygen, temperature, pH, salinity. Strain isolate bacteria were isolated by Bushnell-Hass medium. The bacterial density result shows in IN6 shows that bacterial density increased up to 9 × 108 cells/ml at day 2 incubation and decrease up to 1.79 × 108 cells/ml at final incubation, IN8 increased up to 8.6 × 108 cells/ml, then decrease up to 3.57 × 108 cells/ml. While, EX8 increased up to 9.1 × 108 cells/ml, and then decrease up to 3.06 × 108 cells/ml. While, results of degradation diesel oil hydrocarbon show treatment IN6, TPH values showed a decrease in the initial hydrocarbon concentration of 30 ppm to 16 ppm. IN8 TPH value shows a decrease in the initial hydrocarbon concentration of 30 ppm to 16 ppm. EX8 TPH value shows a decrease in the initial hydrocarbon concentration of 30 ppm to 24 ppm. In this experiment was found that Bacillus cereus was effectively degrading of diesel oil and can be agent bacterial for bioremediation.

Copyrights © 2019






Journal Info

Abbrev

rjls

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

Description

Research Journal of Life Science (RJLS) is an open access publishes papers three times a year that emphasize the agriculture, fisheries, animal science, medicine, basic science and food technology. All articles are rigorously reviewed by reviewers. Articles should be created in a language that is ...