Pathological and biochemical changes in intoxication of mindi (Melia azedarach) leaf

Yulvian Sani
Sjamsul Bahri

Jurnal Ilmu Ternak Veteriner
Vol 1, No 2 (1995)

The purpose of this study was to investigate pathological and biochemical changes in intoxication of mindi leaf (Melia azedarach) on skeletal muscle and myocardial tissues . Fifty two male Wistar rats weighing 140 g to 240 g were intoxicated in 20%; 25% and 40% diet of mindi leaf ad libitwn for 25 days . Clinical signs did not appear the intoxication of mindi leaf. Specific pathological changes were not found macroscopically except cachexia and paleness . Skeletal muscle lesions on the other hand, were consistently noted microscopically . There were degeneration, necrosis and fragmentation of muscle fibres ; enlargement of nuclei ; fibrosis; oedema and hyalination . The hyalination was prominent and appeared to be more. progressive two weeks after dosing . Following the skeletal muscle lesions, myocardial showed degeneration, necrosis and fragmentation of fibres ; enlargement of nuclei and hypercellularity . Regeneration of skeletal muscle developed on day-5 after the substitution of mindi diet by normal diet indicated by disappearing of hyalin tissues . But, myocardial appeared to be regenerated two days after the substitution of diet . Enzymatic activities of alanine aminotransferase (ALAI) and aspartate aminotransferase (ASAT) were not affected. Both enzymes in treated animals were lower than the control . The substitution of diet showed an increased of enzymatic activities during the first two weeks of feeding then reduced at the subsequent weeks . However, these enzymes increased on day-3 after substitution and appeared to maximum followed by a reduction of enzymatic activities in week-4 . On the other hand, creatinine phosphokinase (CPK) was affected in which higher level was noted in treated groups than control . Substitution of diet has led to the reduction of cpc level on day-2 and appeared to reach the same level as in control group on day-21 . The control group was not affected either clinically, pathologically or biochemically . It remained normal throughout the experimental period .