In this project, Sequencing batch reactor (SBR) was operated under anaerobic condition during acidogenic phase for high glucose concentration biodegradation. The results show that for COD concentration of 30.000 mg/l the ratio of fill detention time and react time as 2 : 4 (hour/hour) gave the optimum COD removal of 49,2% and for 60.000 mg/l COD the optimum ratio was 2 : 8 (hour/hour) which gave 58,29% COD removal. From the total volatile and production point of view, the optimum condition for both concentrations was 2 : 8 (hour/hour). Biomass concentration was still above 2.000 mg VSS/l, whereas methane was relatively not be able to be detected.
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