Optimization of Total Flavonoids Extraction and Α-Glucosidase Inhibitory Activity from Plectranthus amboinicus (Lour.) Spreng. Leaves using the Simplex-Centroid Design

Kunto Wibisono (Department of Agronomy and Horticulture, Faculty of Agronomy, Bogor Agricultural University, Bogor, Indonesia)
Syarifah Iis Aisyah (Department of Agronomy and Horticulture, Faculty of Agronomy, Bogor Agricultural University, Bogor, Indonesia)
Sri Suhesti (Plantation Research and Development Center, Agricultural Research Agency, Ministry of Agriculture, Bogor, Indonesia)
Waras Nurcholis (Institut Pertanian Bogor (Bogor Agricultural University))

Molekul: Jurnal Ilmiah Kimia
Vol 14, No 2 (2019)

The leaves of Plectranthus amboinicus (Lour.) Spreng. (Lamiaceae) is a perennial plant that contained flavonoids compounds with have antidiabetic properties. The aim of the work was to select the best solvent for extraction of the flavonoid compounds from torbangun leaves. A statistical simplex-centroid mixture design for the water, ethanol, and hexane solvents has been used to the extraction of extract yield, flavonoids content, and inhibitory α-glucosidase activity of torbangun leaves.  The ethanol-water extracts showed the highest extract yield with value of 15.92%. The ethanol-hexane extracts presented the highest total flavonoids content, 96.30 mg QE/g, followed by hexane extract (82.91 mg QE/g). The ethanol extract recorded higher inhibitory of α-glucosidase activity, with value of 25.53%, followed by hexane extract (25.21%), and ethanol-water extract (23.02%). The weak correlation between the α-glucosidase inhibition and total flavonoids content showed that flavonoids contribute non-significantly to the α-glucosidase activity.