Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Vol. 30 No. 1 (2002): Buletin Agronomi

The Study of Genetic Diversity and Relationships on Carica sp. by Means of Random Amplified Polymorphic DNA (RAPD) Analysis

Ahmad Satori (Unknown)
Sriani Sujiprihati (Unknown)
, Sobir (Unknown)



Article Info

Publish Date
01 Jul 2010

Abstract

The genus of Carica comprises 21 species, among them three species have been domesticated and cultivated. They are Carica papaya, Carica candamarcencis and Carica monoica. In Indonesia, two species have been cultivated viz. Carica papaya and Carica candamarcencis. In this study, four accessions of Carica papaya species and one accession of Carica candamarcencis species were analyzed using RAPD technique. We successfully amplified a total I of 40 fragments from these DNA genome by using 6 random primers with GC bases content:::: 60% of each primer. The number of fragments of each primer ranged from 5 to 8 averaged 6.7 fragments per primer. Out of total fragments, 90% showed as polymorphic ranged from 5 to 8 on average 6 fragments per primer. A dendogram based on the UPGMA- link method using Nei and Li similarity and Principal Component Analysis (PCA) plot separated the accessions into two main groups, the Carica papaya species on the one side and the Carica candamarcencis species on the other side. The result demonstrated that RAPD analysis was able to reveal genetic difference between Carica papaya and Carica candamarcencis species, as well as genetic diversity in Carica papaya species.

Copyrights © 2002






Journal Info

Abbrev

jurnalagronomi

Publisher

Subject

Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

Description

Aims and Scope Aims Indonesian of Journal Agronomy (Jurnal Agronomi Indonesia), the official journal of the Indonesian Society for Agronomy, publishes primary research paper, review article, policy analysis and research notes and preliminary results in all areas of agronomy. Manuscripts could be ...